Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze)
<i>Colletotrichum gloeosporioides</i> and <i>Pseudopestalotiopsis camelliae-sinensis</i> are the two most important tea plant (<i>Camellia sinensis</i> L.) pathogenic fungi. Interest in natural plant extracts as alternatives to synthetic chemical fungicides to con...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/9/1119 |
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| author | Hao Jiang Mengting Zhang Li Qin Dongxu Wang Feng Yu Wenhui Liang Chuankui Song Daniel Granato |
| author_facet | Hao Jiang Mengting Zhang Li Qin Dongxu Wang Feng Yu Wenhui Liang Chuankui Song Daniel Granato |
| author_sort | Hao Jiang |
| collection | DOAJ |
| description | <i>Colletotrichum gloeosporioides</i> and <i>Pseudopestalotiopsis camelliae-sinensis</i> are the two most important tea plant (<i>Camellia sinensis</i> L.) pathogenic fungi. Interest in natural plant extracts as alternatives to synthetic chemical fungicides to control plant pathogens is growing. In this study, the volatile fraction of <i>Baeckea frutescens</i> L. was extracted by supercritical fluid extraction (SFE-CO<sub>2</sub>), and its chemical composition was analyzed, and investigated for its antifungal activity against <i>C. gloeosporioides</i> and <i>P. camelliae</i>. The major constituents of the volatile fraction were β-caryophyllene (28.05%), α-caryophyllene (24.02%), δ-cadinene (6.29%) and eucalyptol (5.46%) in <i>B. frutescens</i> SFE-CO<sub>2</sub> extracts. The terpineol, linalool, terpinen-4-ol and eucalyptol showed strong contact antifungal activity against <i>P. camelliae</i> and <i>C. gloeosporioides</i> with median inhibitory concentration (MIC<sub>50</sub>) in the range of 0.69 μL/mL to 2.79 μL/mL and 0.62 μL/mL to 2.18 μL/mL, respectively. Additionally, the volatile fraction had high fumigation antifungal activity against <i>P. camelliae</i> and <i>C. gloeosporioides</i> with an inhibition rate between 20.87% and 92.91%. Terpineol presented the highest antifungal activity in the contact and fumigation toxicity assays. Terpineol, linalool, terpinen-4-ol and eucalyptol were associated with the most active chemical compounds in the volatile fraction against the fungi. The results suggest that <i>B. frutescens</i> SFE-CO<sub>2</sub> extracts are potential ingredients to develop a natural fungicide for control of tea plant pathogens. |
| first_indexed | 2024-03-10T16:43:22Z |
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| language | English |
| last_indexed | 2024-03-10T16:43:22Z |
| publishDate | 2020-08-01 |
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| spelling | doaj.art-e15531e83531479484d9af51eff9d9e42023-11-20T11:52:00ZengMDPI AGPlants2223-77472020-08-0199111910.3390/plants9091119Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze)Hao Jiang0Mengting Zhang1Li Qin2Dongxu Wang3Feng Yu4Wenhui Liang5Chuankui Song6Daniel Granato7State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaAnhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaSchool of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212003, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaGuangxi Forestry Research Institute, Guangxi Engineering and Technology Research Center for Woody Spices, Guangxi Key Laboratory for Cultivation and Utilization of Special Non-Timber Forest Crops, 23 Yongwu Road, Nanning 530002, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, ChinaFood Processing and Quality, Natural Resources Institute Finland, Tietotie 2, 02150 Espoo, Finland<i>Colletotrichum gloeosporioides</i> and <i>Pseudopestalotiopsis camelliae-sinensis</i> are the two most important tea plant (<i>Camellia sinensis</i> L.) pathogenic fungi. Interest in natural plant extracts as alternatives to synthetic chemical fungicides to control plant pathogens is growing. In this study, the volatile fraction of <i>Baeckea frutescens</i> L. was extracted by supercritical fluid extraction (SFE-CO<sub>2</sub>), and its chemical composition was analyzed, and investigated for its antifungal activity against <i>C. gloeosporioides</i> and <i>P. camelliae</i>. The major constituents of the volatile fraction were β-caryophyllene (28.05%), α-caryophyllene (24.02%), δ-cadinene (6.29%) and eucalyptol (5.46%) in <i>B. frutescens</i> SFE-CO<sub>2</sub> extracts. The terpineol, linalool, terpinen-4-ol and eucalyptol showed strong contact antifungal activity against <i>P. camelliae</i> and <i>C. gloeosporioides</i> with median inhibitory concentration (MIC<sub>50</sub>) in the range of 0.69 μL/mL to 2.79 μL/mL and 0.62 μL/mL to 2.18 μL/mL, respectively. Additionally, the volatile fraction had high fumigation antifungal activity against <i>P. camelliae</i> and <i>C. gloeosporioides</i> with an inhibition rate between 20.87% and 92.91%. Terpineol presented the highest antifungal activity in the contact and fumigation toxicity assays. Terpineol, linalool, terpinen-4-ol and eucalyptol were associated with the most active chemical compounds in the volatile fraction against the fungi. The results suggest that <i>B. frutescens</i> SFE-CO<sub>2</sub> extracts are potential ingredients to develop a natural fungicide for control of tea plant pathogens.https://www.mdpi.com/2223-7747/9/9/1119<i>Camellia sinensis</i>pathogenic fungi<i>Pseudopestalotiopsis camelliae-sinensis</i>Colletotrichum gloeosporioidessupercritical fluid extractionantifungal activity |
| spellingShingle | Hao Jiang Mengting Zhang Li Qin Dongxu Wang Feng Yu Wenhui Liang Chuankui Song Daniel Granato Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze) Plants <i>Camellia sinensis</i> pathogenic fungi <i>Pseudopestalotiopsis camelliae-sinensis</i> Colletotrichum gloeosporioides supercritical fluid extraction antifungal activity |
| title | Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze) |
| title_full | Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze) |
| title_fullStr | Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze) |
| title_full_unstemmed | Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze) |
| title_short | Chemical Composition of a Supercritical Fluid (Sfe-CO<sub>2</sub>) Extract from <i>Baeckea frutescens</i> L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (<i>Camellia sinensis</i> (L.) O. Kuntze) |
| title_sort | chemical composition of a supercritical fluid sfe co sub 2 sub extract from i baeckea frutescens i l leaves and its bioactivity against two pathogenic fungi isolated from the tea plant i camellia sinensis i l o kuntze |
| topic | <i>Camellia sinensis</i> pathogenic fungi <i>Pseudopestalotiopsis camelliae-sinensis</i> Colletotrichum gloeosporioides supercritical fluid extraction antifungal activity |
| url | https://www.mdpi.com/2223-7747/9/9/1119 |
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