Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering
Stem cells and scaffolds play a very important role in tissue engineering. Here, we isolated synovium-derived mesenchymal stem cells (SMSCs) from synovial membrane tissue and characterized stem-cell properties. Gelatin nanoparticles (NP) were prepared using a two-step desolvation method and then pre...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-07-01
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| Series: | Science and Technology of Advanced Materials |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1088/1468-6996/16/4/045001 |
| _version_ | 1819013287572930560 |
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| author | Jian-Feng Pan Shuo Li Chang-An Guo Du-Liang Xu Feng Zhang Zuo-Qin Yan Xiu-Mei Mo |
| author_facet | Jian-Feng Pan Shuo Li Chang-An Guo Du-Liang Xu Feng Zhang Zuo-Qin Yan Xiu-Mei Mo |
| author_sort | Jian-Feng Pan |
| collection | DOAJ |
| description | Stem cells and scaffolds play a very important role in tissue engineering. Here, we isolated synovium-derived mesenchymal stem cells (SMSCs) from synovial membrane tissue and characterized stem-cell properties. Gelatin nanoparticles (NP) were prepared using a two-step desolvation method and then pre-mixed into different host matrix (silk fibroin (SF), gelatin (Gel), or SF–Gel mixture) to generate various 3D printed nanocomposite scaffolds (NP/SF, NP/SF–Gel, NP/Gel-1, and NP/Gel-2). The microstructure was examined by scanning electron microscopy. Biocompatibility assessment was performed through CCK-8 assay by coculturing with SMSCs at 1, 3, 7 and 14 days. According to the results, SMSCs are similar to other MSCs in their surface epitope expression, which are negative for CD45 and positive for CD44, CD90, and CD105. After incubation in lineage-specific medium, SMSCs could differentiate into chondrocytes, osteocytes and adipocytes. 3D printed nanocomposite scaffolds exhibited a good biocompatibility in the process of coculturing with SMSCs and had no negative effect on cell behavior. The study provides a strategy to obtain SMSCs and fabricate 3D printed nanocomposite scaffolds, the combination of which could be used for practical applications in tissue engineering. |
| first_indexed | 2024-12-21T01:57:33Z |
| format | Article |
| id | doaj.art-e1589879406e455d9d314b2cabe2ffee |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-12-21T01:57:33Z |
| publishDate | 2015-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-e1589879406e455d9d314b2cabe2ffee2022-12-21T19:19:44ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142015-07-0116410.1088/1468-6996/16/4/04500111661316Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineeringJian-Feng Pan0Shuo Li1Chang-An Guo2Du-Liang Xu3Feng Zhang4Zuo-Qin Yan5Xiu-Mei Mo6Zhongshan Hospital of Fudan UniversityZhongshan Hospital of Fudan UniversityZhongshan Hospital of Fudan UniversityCollege of Materials Science and Engineering, Donghua UniversityZhongshan Hospital of Fudan UniversityZhongshan Hospital of Fudan UniversityCollege of Materials Science and Engineering, Donghua UniversityStem cells and scaffolds play a very important role in tissue engineering. Here, we isolated synovium-derived mesenchymal stem cells (SMSCs) from synovial membrane tissue and characterized stem-cell properties. Gelatin nanoparticles (NP) were prepared using a two-step desolvation method and then pre-mixed into different host matrix (silk fibroin (SF), gelatin (Gel), or SF–Gel mixture) to generate various 3D printed nanocomposite scaffolds (NP/SF, NP/SF–Gel, NP/Gel-1, and NP/Gel-2). The microstructure was examined by scanning electron microscopy. Biocompatibility assessment was performed through CCK-8 assay by coculturing with SMSCs at 1, 3, 7 and 14 days. According to the results, SMSCs are similar to other MSCs in their surface epitope expression, which are negative for CD45 and positive for CD44, CD90, and CD105. After incubation in lineage-specific medium, SMSCs could differentiate into chondrocytes, osteocytes and adipocytes. 3D printed nanocomposite scaffolds exhibited a good biocompatibility in the process of coculturing with SMSCs and had no negative effect on cell behavior. The study provides a strategy to obtain SMSCs and fabricate 3D printed nanocomposite scaffolds, the combination of which could be used for practical applications in tissue engineering.http://dx.doi.org/10.1088/1468-6996/16/4/045001synovium-derived mesenchymal stem cellsnanoparticles3d printed nanocomposite scaffolds |
| spellingShingle | Jian-Feng Pan Shuo Li Chang-An Guo Du-Liang Xu Feng Zhang Zuo-Qin Yan Xiu-Mei Mo Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering Science and Technology of Advanced Materials synovium-derived mesenchymal stem cells nanoparticles 3d printed nanocomposite scaffolds |
| title | Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering |
| title_full | Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering |
| title_fullStr | Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering |
| title_full_unstemmed | Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering |
| title_short | Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering |
| title_sort | evaluation of synovium derived mesenchymal stem cells and 3d printed nanocomposite scaffolds for tissue engineering |
| topic | synovium-derived mesenchymal stem cells nanoparticles 3d printed nanocomposite scaffolds |
| url | http://dx.doi.org/10.1088/1468-6996/16/4/045001 |
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