A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i>
Concentrated bud macerates (CBMs) are obtained from meristematic tissues such as buds and young shoots by maceration in a solvent composed of glycerin, water and ethanol (1/1/1/, <i>v</i>/<i>v</i>). Their traditional utilization in gemmotherapy has gained interest in the past...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-01-01
|
Series: | Plants |
Subjects: | |
Online Access: | https://www.mdpi.com/2223-7747/11/2/144 |
_version_ | 1797491037702193152 |
---|---|
author | Thomas Charpentier Séverine Boisard Anne-Marie Le Ray Dimitri Bréard Amélie Chabrier Hélène Esselin David Guilet Christophe Ripoll Pascal Richomme |
author_facet | Thomas Charpentier Séverine Boisard Anne-Marie Le Ray Dimitri Bréard Amélie Chabrier Hélène Esselin David Guilet Christophe Ripoll Pascal Richomme |
author_sort | Thomas Charpentier |
collection | DOAJ |
description | Concentrated bud macerates (CBMs) are obtained from meristematic tissues such as buds and young shoots by maceration in a solvent composed of glycerin, water and ethanol (1/1/1/, <i>v</i>/<i>v</i>). Their traditional utilization in gemmotherapy has gained interest in the past years, and the knowledge of their chemical characterization can provide commercial arguments, particularly to secure their quality control. Therefore, an optimized method for phytochemical analysis including glycerol removal by a preliminary solid phase extraction (SPE) followed by compound identification using high performance liquid chromatography coupled with ultra-violet and tandem mass detectors (HPLC-UV-MS<sup>2</sup>) was developed. This method was applied on 5 CBMs obtained from <i>Alnus glutinosa</i>, <i>Ribes</i><i>nigrum</i>, <i>Rosmarinus officinalis</i>, <i>Rosa canina</i> and <i>Tilia tomentosa</i> in order to determinate their chemical composition. Their antioxidant effects were also investigated by radical scavenging activity assays (DPPH and ORAC). Glycerol removal improved the resolution of HPLC chemical profiles and allowed us to perform TLC antioxidant screening. Our approach permitted the identification of 57 compounds distributed in eight major classes, three of them being common to all macerates including nucleosides, phenolic acids and glycosylated flavonoids. Quantification of the later class as a rutin equivalent (RE) showed a great disparity between <i>Rosa canina</i> macerate (809 mg RE/L), and the other ones (from 175 to 470 mg RE/L). DPPH and ORAC assays confirmed the great activity of <i>Rosa canina</i> (4857 and 6479 μmol TE/g of dry matter, respectively). Finally, phytochemical and antioxidant analysis of CBMs strengthened their phytomedicinal interest in the gemmotherapy field. |
first_indexed | 2024-03-10T00:41:41Z |
format | Article |
id | doaj.art-e15927b05c11444393f87f6d46e69068 |
institution | Directory Open Access Journal |
issn | 2223-7747 |
language | English |
last_indexed | 2024-03-10T00:41:41Z |
publishDate | 2022-01-01 |
publisher | MDPI AG |
record_format | Article |
series | Plants |
spelling | doaj.art-e15927b05c11444393f87f6d46e690682023-11-23T15:06:35ZengMDPI AGPlants2223-77472022-01-0111214410.3390/plants11020144A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i>Thomas Charpentier0Séverine Boisard1Anne-Marie Le Ray2Dimitri Bréard3Amélie Chabrier4Hélène Esselin5David Guilet6Christophe Ripoll7Pascal Richomme8EA921 SONAS, SFR4207 QUASAV, Campus du Vegetal, University of Angers, 49070 Beaucouzé, FranceEA921 SONAS, SFR4207 QUASAV, Campus du Vegetal, University of Angers, 49070 Beaucouzé, FranceEA921 SONAS, SFR4207 QUASAV, Campus du Vegetal, University of Angers, 49070 Beaucouzé, FranceEA921 SONAS, SFR4207 QUASAV, Campus du Vegetal, University of Angers, 49070 Beaucouzé, FranceNatinov, ZA Montendre, St Lézin, 49120 Chemillé en Anjou, FranceNatinov, ZA Montendre, St Lézin, 49120 Chemillé en Anjou, FranceEA921 SONAS, SFR4207 QUASAV, Campus du Vegetal, University of Angers, 49070 Beaucouzé, FranceNatinov, ZA Montendre, St Lézin, 49120 Chemillé en Anjou, FranceEA921 SONAS, SFR4207 QUASAV, Campus du Vegetal, University of Angers, 49070 Beaucouzé, FranceConcentrated bud macerates (CBMs) are obtained from meristematic tissues such as buds and young shoots by maceration in a solvent composed of glycerin, water and ethanol (1/1/1/, <i>v</i>/<i>v</i>). Their traditional utilization in gemmotherapy has gained interest in the past years, and the knowledge of their chemical characterization can provide commercial arguments, particularly to secure their quality control. Therefore, an optimized method for phytochemical analysis including glycerol removal by a preliminary solid phase extraction (SPE) followed by compound identification using high performance liquid chromatography coupled with ultra-violet and tandem mass detectors (HPLC-UV-MS<sup>2</sup>) was developed. This method was applied on 5 CBMs obtained from <i>Alnus glutinosa</i>, <i>Ribes</i><i>nigrum</i>, <i>Rosmarinus officinalis</i>, <i>Rosa canina</i> and <i>Tilia tomentosa</i> in order to determinate their chemical composition. Their antioxidant effects were also investigated by radical scavenging activity assays (DPPH and ORAC). Glycerol removal improved the resolution of HPLC chemical profiles and allowed us to perform TLC antioxidant screening. Our approach permitted the identification of 57 compounds distributed in eight major classes, three of them being common to all macerates including nucleosides, phenolic acids and glycosylated flavonoids. Quantification of the later class as a rutin equivalent (RE) showed a great disparity between <i>Rosa canina</i> macerate (809 mg RE/L), and the other ones (from 175 to 470 mg RE/L). DPPH and ORAC assays confirmed the great activity of <i>Rosa canina</i> (4857 and 6479 μmol TE/g of dry matter, respectively). Finally, phytochemical and antioxidant analysis of CBMs strengthened their phytomedicinal interest in the gemmotherapy field.https://www.mdpi.com/2223-7747/11/2/144concentrated bud maceratesHPLC-UV-MSflavonoid contentantioxidant activity |
spellingShingle | Thomas Charpentier Séverine Boisard Anne-Marie Le Ray Dimitri Bréard Amélie Chabrier Hélène Esselin David Guilet Christophe Ripoll Pascal Richomme A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i> Plants concentrated bud macerates HPLC-UV-MS flavonoid content antioxidant activity |
title | A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i> |
title_full | A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i> |
title_fullStr | A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i> |
title_full_unstemmed | A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i> |
title_short | A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS<sup>2</sup> Method—Application to <i>Alnus glutinosa</i>, <i>Ribes nigrum</i>, <i>Rosa canina</i>, <i>Rosmarinus officinalis</i> and <i>Tilia tomentosa</i> |
title_sort | descriptive chemical composition of concentrated bud macerates through an optimized spe hplc uv ms sup 2 sup method application to i alnus glutinosa i i ribes nigrum i i rosa canina i i rosmarinus officinalis i and i tilia tomentosa i |
topic | concentrated bud macerates HPLC-UV-MS flavonoid content antioxidant activity |
url | https://www.mdpi.com/2223-7747/11/2/144 |
work_keys_str_mv | AT thomascharpentier adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT severineboisard adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT annemarieleray adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT dimitribreard adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT ameliechabrier adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT heleneesselin adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT davidguilet adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT christopheripoll adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT pascalrichomme adescriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT thomascharpentier descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT severineboisard descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT annemarieleray descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT dimitribreard descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT ameliechabrier descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT heleneesselin descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT davidguilet descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT christopheripoll descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai AT pascalrichomme descriptivechemicalcompositionofconcentratedbudmaceratesthroughanoptimizedspehplcuvmssup2supmethodapplicationtoialnusglutinosaiiribesnigrumiirosacaninaiirosmarinusofficinalisianditiliatomentosai |