Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital
Background We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods One hundred samples were selected to evaluate the quality of glioma sam...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2018-03-01
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| Online Access: | https://peerj.com/articles/4450.pdf |
| _version_ | 1797418493962878976 |
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| author | Fanhong Kong Wenli Zhang Lin Qiao Qi Li Haowen Li Jingli Cao Wenyan He Chengya Dong Yanjiao He Lu He Li Liu Weilun Fu Lijun Liu Zirui Li Yajie Wang |
| author_facet | Fanhong Kong Wenli Zhang Lin Qiao Qi Li Haowen Li Jingli Cao Wenyan He Chengya Dong Yanjiao He Lu He Li Liu Weilun Fu Lijun Liu Zirui Li Yajie Wang |
| author_sort | Fanhong Kong |
| collection | DOAJ |
| description | Background We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination. Results The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation. Conclusion Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality. |
| first_indexed | 2024-03-09T06:34:38Z |
| format | Article |
| id | doaj.art-e15940f34f404b7592b8c1fef102be42 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:34:38Z |
| publishDate | 2018-03-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-e15940f34f404b7592b8c1fef102be422023-12-03T11:01:18ZengPeerJ Inc.PeerJ2167-83592018-03-016e445010.7717/peerj.4450Establishment and quality evaluation of a glioma biobank in Beijing Tiantan HospitalFanhong Kong0Wenli Zhang1Lin Qiao2Qi Li3Haowen Li4Jingli Cao5Wenyan He6Chengya Dong7Yanjiao He8Lu He9Li Liu10Weilun Fu11Lijun Liu12Zirui Li13Yajie Wang14Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaBeijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaNeuropathological department, Beijing Neurosugical Institute, Capital Medical University, Beijing, ChinaBeijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaBeijing Tiantan Hospital, Capital Medical University, Beijing, ChinaDepartment of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USAClinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaClinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing, ChinaBackground We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination. Results The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation. Conclusion Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.https://peerj.com/articles/4450.pdfGliomaBiobankQuality evaluation |
| spellingShingle | Fanhong Kong Wenli Zhang Lin Qiao Qi Li Haowen Li Jingli Cao Wenyan He Chengya Dong Yanjiao He Lu He Li Liu Weilun Fu Lijun Liu Zirui Li Yajie Wang Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital PeerJ Glioma Biobank Quality evaluation |
| title | Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital |
| title_full | Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital |
| title_fullStr | Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital |
| title_full_unstemmed | Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital |
| title_short | Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital |
| title_sort | establishment and quality evaluation of a glioma biobank in beijing tiantan hospital |
| topic | Glioma Biobank Quality evaluation |
| url | https://peerj.com/articles/4450.pdf |
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