Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization
Summary: Differentiated cardiomyocytes (CMs) must undergo diverse morphological and functional changes during postnatal development. However, the mechanisms underlying initiation and coordination of these changes remain unclear. Here, we delineate an integrated, time-ordered transcriptional network...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723014286 |
| _version_ | 1797448085700345856 |
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| author | Chia-Yeh Lin Yao-Ming Chang Hsin-Yi Tseng Yen-Ling Shih Hsiao-Hui Yeh You-Rou Liao Han-Hsuan Tang Chia-Ling Hsu Chien-Chang Chen Yu-Ting Yan Cheng-Fu Kao |
| author_facet | Chia-Yeh Lin Yao-Ming Chang Hsin-Yi Tseng Yen-Ling Shih Hsiao-Hui Yeh You-Rou Liao Han-Hsuan Tang Chia-Ling Hsu Chien-Chang Chen Yu-Ting Yan Cheng-Fu Kao |
| author_sort | Chia-Yeh Lin |
| collection | DOAJ |
| description | Summary: Differentiated cardiomyocytes (CMs) must undergo diverse morphological and functional changes during postnatal development. However, the mechanisms underlying initiation and coordination of these changes remain unclear. Here, we delineate an integrated, time-ordered transcriptional network that begins with expression of genes for cell-cell connections and leads to a sequence of structural, cell-cycle, functional, and metabolic transitions in mouse postnatal hearts. Depletion of histone H2B ubiquitin ligase RNF20 disrupts this gene network and impairs CM polarization. Subsequently, assay for transposase-accessible chromatin using sequencing (ATAC-seq) analysis confirmed that RNF20 contributes to chromatin accessibility in this context. As such, RNF20 is likely to facilitate binding of transcription factors at the promoters of genes involved in cell-cell connections and actin organization, which are crucial for CM polarization and functional integration. These results suggest that CM polarization is one of the earliest events during postnatal heart development and provide insights into how RNF20 regulates CM polarity and the postnatal gene program. |
| first_indexed | 2024-03-09T14:05:16Z |
| format | Article |
| id | doaj.art-e159d2452509443bb54e6e0e7ef957b1 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-09T14:05:16Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e159d2452509443bb54e6e0e7ef957b12023-11-30T05:07:09ZengElsevierCell Reports2211-12472023-11-014211113416Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarizationChia-Yeh Lin0Yao-Ming Chang1Hsin-Yi Tseng2Yen-Ling Shih3Hsiao-Hui Yeh4You-Rou Liao5Han-Hsuan Tang6Chia-Ling Hsu7Chien-Chang Chen8Yu-Ting Yan9Cheng-Fu Kao10Institute of Cellular and Organismic Biology, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Biomedical Sciences, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Cellular and Organismic Biology, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Biomedical Sciences, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Biomedical Sciences, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Cellular and Organismic Biology, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Cellular and Organismic Biology, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Cellular and Organismic Biology, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Biomedical Sciences, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, TaiwanInstitute of Biomedical Sciences, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, Taiwan; Corresponding authorInstitute of Cellular and Organismic Biology, Academia Sinica, 128, Academia Road, Section 2, Nankang, Taipei, Taiwan; Corresponding authorSummary: Differentiated cardiomyocytes (CMs) must undergo diverse morphological and functional changes during postnatal development. However, the mechanisms underlying initiation and coordination of these changes remain unclear. Here, we delineate an integrated, time-ordered transcriptional network that begins with expression of genes for cell-cell connections and leads to a sequence of structural, cell-cycle, functional, and metabolic transitions in mouse postnatal hearts. Depletion of histone H2B ubiquitin ligase RNF20 disrupts this gene network and impairs CM polarization. Subsequently, assay for transposase-accessible chromatin using sequencing (ATAC-seq) analysis confirmed that RNF20 contributes to chromatin accessibility in this context. As such, RNF20 is likely to facilitate binding of transcription factors at the promoters of genes involved in cell-cell connections and actin organization, which are crucial for CM polarization and functional integration. These results suggest that CM polarization is one of the earliest events during postnatal heart development and provide insights into how RNF20 regulates CM polarity and the postnatal gene program.http://www.sciencedirect.com/science/article/pii/S2211124723014286CP: Molecular biologyCP: Developmental biology |
| spellingShingle | Chia-Yeh Lin Yao-Ming Chang Hsin-Yi Tseng Yen-Ling Shih Hsiao-Hui Yeh You-Rou Liao Han-Hsuan Tang Chia-Ling Hsu Chien-Chang Chen Yu-Ting Yan Cheng-Fu Kao Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization Cell Reports CP: Molecular biology CP: Developmental biology |
| title | Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization |
| title_full | Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization |
| title_fullStr | Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization |
| title_full_unstemmed | Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization |
| title_short | Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization |
| title_sort | epigenetic regulator rnf20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization |
| topic | CP: Molecular biology CP: Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723014286 |
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