Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction
Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively...
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| Format: | Article |
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MDPI AG
2017-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/4/3/72 |
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| author | Ying Zhang Kin Liao Chuan Li Alvin C.K. Lai Ji-Jinn Foo Vincent Chan |
| author_facet | Ying Zhang Kin Liao Chuan Li Alvin C.K. Lai Ji-Jinn Foo Vincent Chan |
| author_sort | Ying Zhang |
| collection | DOAJ |
| description | Mechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell–cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions. |
| first_indexed | 2024-03-12T20:25:51Z |
| format | Article |
| id | doaj.art-e15ae01303824d93a275e4bad0a85b73 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T20:25:51Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj.art-e15ae01303824d93a275e4bad0a85b732023-08-02T00:31:22ZengMDPI AGBioengineering2306-53542017-08-01437210.3390/bioengineering4030072bioengineering4030072Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell MechanotransductionYing Zhang0Kin Liao1Chuan Li2Alvin C.K. Lai3Ji-Jinn Foo4Vincent Chan5Department of Chemical Engineering, Khalifa University, Abu Dhabi 127788, UAEDepartment of Aerospace Engineering, Khalifa University, Abu Dhabi 127788, UAEDepartment of Biomedical Engineering, National Yang Ming University, Taipei 11221, TaiwanDepartment of Architecture and Civil Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong KongSchool of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, MalaysiaDepartment of Chemical Engineering, Khalifa University, Abu Dhabi 127788, UAEMechanotransduction between cells and the extracellular matrix regulates major cellular functions in physiological and pathological situations. The effect of mechanical cues on biochemical signaling triggered by cell–matrix and cell–cell interactions on model biomimetic surfaces has been extensively investigated by a combination of fabrication, biophysical, and biological methods. To simulate the in vivo physiological microenvironment in vitro, three dimensional (3D) microstructures with tailored bio-functionality have been fabricated on substrates of various materials. However, less attention has been paid to the design of 3D biomaterial systems with geometric variances, such as the possession of precise micro-features and/or bio-sensing elements for probing the mechanical responses of cells to the external microenvironment. Such precisely engineered 3D model experimental platforms pave the way for studying the mechanotransduction of multicellular aggregates under controlled geometric and mechanical parameters. Concurrently with the progress in 3D biomaterial fabrication, cell traction force microscopy (CTFM) developed in the field of cell biophysics has emerged as a highly sensitive technique for probing the mechanical stresses exerted by cells onto the opposing deformable surface. In the current work, we first review the recent advances in the fabrication of 3D micropatterned biomaterials which enable the seamless integration with experimental cell mechanics in a controlled 3D microenvironment. Then, we discuss the role of collective cell–cell interactions in the mechanotransduction of engineered tissue equivalents determined by such integrative biomaterial systems under simulated physiological conditions.https://www.mdpi.com/2306-5354/4/3/72mechanotransductionsoft lithographycell-matrix interactionscell–cell interactionscell traction force microscopy3D tissue mechanics |
| spellingShingle | Ying Zhang Kin Liao Chuan Li Alvin C.K. Lai Ji-Jinn Foo Vincent Chan Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction Bioengineering mechanotransduction soft lithography cell-matrix interactions cell–cell interactions cell traction force microscopy 3D tissue mechanics |
| title | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
| title_full | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
| title_fullStr | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
| title_full_unstemmed | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
| title_short | Progress in Integrative Biomaterial Systems to Approach Three-Dimensional Cell Mechanotransduction |
| title_sort | progress in integrative biomaterial systems to approach three dimensional cell mechanotransduction |
| topic | mechanotransduction soft lithography cell-matrix interactions cell–cell interactions cell traction force microscopy 3D tissue mechanics |
| url | https://www.mdpi.com/2306-5354/4/3/72 |
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