Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy
Summary: In rats and guinea pigs, sensory innervation of the airways is derived largely from the vagus nerve, with the extrapulmonary airways innervated by Wnt1+ jugular neurons and the intrapulmonary airways and lungs by Phox2b+ nodose neurons; however, our knowledge of airway innervation in mice i...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224004036 |
| _version_ | 1797300393124823040 |
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| author | Aung Aung Kywe Moe Tara G. Bautista Matthew W. Trewella Willian S. Korim Song T. Yao Robert Behrens Alexandria K. Driessen Alice E. McGovern Stuart B. Mazzone |
| author_facet | Aung Aung Kywe Moe Tara G. Bautista Matthew W. Trewella Willian S. Korim Song T. Yao Robert Behrens Alexandria K. Driessen Alice E. McGovern Stuart B. Mazzone |
| author_sort | Aung Aung Kywe Moe |
| collection | DOAJ |
| description | Summary: In rats and guinea pigs, sensory innervation of the airways is derived largely from the vagus nerve, with the extrapulmonary airways innervated by Wnt1+ jugular neurons and the intrapulmonary airways and lungs by Phox2b+ nodose neurons; however, our knowledge of airway innervation in mice is limited. We used genetically targeted expression of enhanced yellow fluorescent protein-channelrhodopsin-2 (EYFP-ChR2) in Wnt1+ or Phox2b+ tissues to characterize jugular and nodose-mediated physiological responses and airway innervation in mice. With optical stimulation, Phox2b+ vagal fibers modulated cardiorespiratory function in a frequency-dependent manner while right Wnt1+ vagal fibers induced a small increase in respiratory rate. Mouse tracheae contained sparse Phox2b-EYFP fibers but dense networks of Wnt1-EYFP fibers. Retrograde tracing from the airways showed limited tracheal innervation by the jugular sensory neurons, distinct from other species. These differences in physiology and vagal sensory distribution have important implications when using mice for studying airway neurobiology. |
| first_indexed | 2024-03-07T23:06:32Z |
| format | Article |
| id | doaj.art-e15b5edbab184ff68b7cccaff585fa0c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-07T23:06:32Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e15b5edbab184ff68b7cccaff585fa0c2024-02-22T04:53:14ZengElsevieriScience2589-00422024-03-01273109182Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomyAung Aung Kywe Moe0Tara G. Bautista1Matthew W. Trewella2Willian S. Korim3Song T. Yao4Robert Behrens5Alexandria K. Driessen6Alice E. McGovern7Stuart B. Mazzone8Department of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, Australia; Department of Medical Imaging and Radiation Sciences, School of Primary and Allied Health Care, Monash University, Clayton Campus, Clayton, VIC 3800, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Parkville, VIC 3010, Australia; Corresponding authorSummary: In rats and guinea pigs, sensory innervation of the airways is derived largely from the vagus nerve, with the extrapulmonary airways innervated by Wnt1+ jugular neurons and the intrapulmonary airways and lungs by Phox2b+ nodose neurons; however, our knowledge of airway innervation in mice is limited. We used genetically targeted expression of enhanced yellow fluorescent protein-channelrhodopsin-2 (EYFP-ChR2) in Wnt1+ or Phox2b+ tissues to characterize jugular and nodose-mediated physiological responses and airway innervation in mice. With optical stimulation, Phox2b+ vagal fibers modulated cardiorespiratory function in a frequency-dependent manner while right Wnt1+ vagal fibers induced a small increase in respiratory rate. Mouse tracheae contained sparse Phox2b-EYFP fibers but dense networks of Wnt1-EYFP fibers. Retrograde tracing from the airways showed limited tracheal innervation by the jugular sensory neurons, distinct from other species. These differences in physiology and vagal sensory distribution have important implications when using mice for studying airway neurobiology.http://www.sciencedirect.com/science/article/pii/S2589004224004036Sensory neuroscienceTechniques in neuroscience |
| spellingShingle | Aung Aung Kywe Moe Tara G. Bautista Matthew W. Trewella Willian S. Korim Song T. Yao Robert Behrens Alexandria K. Driessen Alice E. McGovern Stuart B. Mazzone Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy iScience Sensory neuroscience Techniques in neuroscience |
| title | Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy |
| title_full | Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy |
| title_fullStr | Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy |
| title_full_unstemmed | Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy |
| title_short | Investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy |
| title_sort | investigation of vagal sensory neurons in mice using optical vagal stimulation and tracheal neuroanatomy |
| topic | Sensory neuroscience Techniques in neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224004036 |
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