Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Microbiology
2010-08-01
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| Series: | Microbiology Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/63 |
| _version_ | 1819062975306137600 |
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| author | DERI YURATMOKO NISA RACHMANIA MUBARIK ANJA MERYANDINI |
| author_facet | DERI YURATMOKO NISA RACHMANIA MUBARIK ANJA MERYANDINI |
| author_sort | DERI YURATMOKO |
| collection | DOAJ |
| description | Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 ºC, whereas that of the keratinase was at pH 6.5 and 70 +C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 ºC whereas the keratinase was at pH 8.5 and 80 ºC. |
| first_indexed | 2024-12-21T15:07:19Z |
| format | Article |
| id | doaj.art-e15ec1710fec4671a5ece3777662a9c3 |
| institution | Directory Open Access Journal |
| issn | 1978-3477 2087-8575 |
| language | English |
| last_indexed | 2024-12-21T15:07:19Z |
| publishDate | 2010-08-01 |
| publisher | Indonesian Society for Microbiology |
| record_format | Article |
| series | Microbiology Indonesia |
| spelling | doaj.art-e15ec1710fec4671a5ece3777662a9c32022-12-21T18:59:24ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752010-08-011210.5454/mi.1.2.559Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their CharacterizationDERI YURATMOKONISA RACHMANIA MUBARIKANJA MERYANDINIProtease of two Streptomyces sp. strain were chosen for characterization because of the large clear zone surrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 ºC, whereas that of the keratinase was at pH 6.5 and 70 +C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 ºC whereas the keratinase was at pH 8.5 and 80 ºC.https://jurnal.permi.or.id/index.php/mionline/article/view/63Streptomyces sp.proteasekeratinase |
| spellingShingle | DERI YURATMOKO NISA RACHMANIA MUBARIK ANJA MERYANDINI Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization Microbiology Indonesia Streptomyces sp. protease keratinase |
| title | Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization |
| title_full | Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization |
| title_fullStr | Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization |
| title_full_unstemmed | Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization |
| title_short | Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization |
| title_sort | screening of proteolytic enzymes of streptomyces sp local strain and their characterization |
| topic | Streptomyces sp. protease keratinase |
| url | https://jurnal.permi.or.id/index.php/mionline/article/view/63 |
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