Efficacy of Four Disinfectants Against Hepatitis B Virus
Background and Aim: Hepatitis B virus (HBV) is an important infectious agent in denti-stry necessitating the use of disinfectants to prevent its transmission. This study compared the efficacy of 2/100 sodium hypochlorite disinfectants prepared from 5% solution manufactured by four different manufact...
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| Format: | Article |
| Language: | English |
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Islamic Dental Association of Iran
2015-04-01
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| Series: | Journal of Islamic Dental Association of Iran |
| Subjects: | |
| Online Access: | http://jidai.ir/browse.php?a_code=A-10-1-821&slc_lang=en&sid=1 |
| _version_ | 1819055556386619392 |
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| author | Mohammad reza Aghasadeghi Sakine Arami Masomeh Hasani Tabatabaie Hojjat Darvishpour Mohammad javad Kharazifard Hamid Sarlak |
| author_facet | Mohammad reza Aghasadeghi Sakine Arami Masomeh Hasani Tabatabaie Hojjat Darvishpour Mohammad javad Kharazifard Hamid Sarlak |
| author_sort | Mohammad reza Aghasadeghi |
| collection | DOAJ |
| description | Background and Aim: Hepatitis B virus (HBV) is an important infectious agent in denti-stry necessitating the use of disinfectants to prevent its transmission. This study compared the efficacy of 2/100 sodium hypochlorite disinfectants prepared from 5% solution manufactured by four different manufacturers namely Ashimashi, Paknaz, Vitex and Active for disinfection of surfaces infected with HBV.
Materials and Methods: In this in vitro experimental study, sera of 10 hepatitis B pa-tients were poured into microtubes and 2/100 sodium hypochlorite solutions prepared from 5% Ashimashi, Paknaz, Vitex and Active disinfectants were added to them in 1/1 ratio. Polymerase chain reaction (PCR) was performed using virus diagnostic kits to detect the viral genome. Real-time PCR was performed before and after incubation with the disinfectants to assess the viral load in the serum. The reduction in the viral load of HBV was statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests.
Results: No significant antiviral efficacy was noted following the application of Ashima-shi 2/100 sodium hypochlorite disinfectant. Paknaz showed the highest efficacy against HBV. Vitex and Active ranked next with significant differences (P<0.0001)
Conclusion: Under the study limitations, Paknaz 2/100 sodium hypochlorite solution was the most effective while Ashimashi 2/100 sodium hypochlorite disinfectant did not show adequate efficacy against HBV. |
| first_indexed | 2024-12-21T13:09:24Z |
| format | Article |
| id | doaj.art-e15f100a72a741bb8d468eeeff461607 |
| institution | Directory Open Access Journal |
| issn | 2383-3041 2383-3041 |
| language | English |
| last_indexed | 2024-12-21T13:09:24Z |
| publishDate | 2015-04-01 |
| publisher | Islamic Dental Association of Iran |
| record_format | Article |
| series | Journal of Islamic Dental Association of Iran |
| spelling | doaj.art-e15f100a72a741bb8d468eeeff4616072022-12-21T19:02:56ZengIslamic Dental Association of IranJournal of Islamic Dental Association of Iran2383-30412383-30412015-04-012728589Efficacy of Four Disinfectants Against Hepatitis B VirusMohammad reza Aghasadeghi0Sakine Arami1Masomeh Hasani Tabatabaie2Hojjat Darvishpour3Mohammad javad Kharazifard4Hamid Sarlak5 Associate Professor, Department of Virology, Pasteur Institute of Iran, Tehran, Iran Assistant Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences AND Department of Operative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Associate Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences AND Department of Operative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Postgraduate Student, Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Research Member of Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran Postgraduate Student, Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran Background and Aim: Hepatitis B virus (HBV) is an important infectious agent in denti-stry necessitating the use of disinfectants to prevent its transmission. This study compared the efficacy of 2/100 sodium hypochlorite disinfectants prepared from 5% solution manufactured by four different manufacturers namely Ashimashi, Paknaz, Vitex and Active for disinfection of surfaces infected with HBV. Materials and Methods: In this in vitro experimental study, sera of 10 hepatitis B pa-tients were poured into microtubes and 2/100 sodium hypochlorite solutions prepared from 5% Ashimashi, Paknaz, Vitex and Active disinfectants were added to them in 1/1 ratio. Polymerase chain reaction (PCR) was performed using virus diagnostic kits to detect the viral genome. Real-time PCR was performed before and after incubation with the disinfectants to assess the viral load in the serum. The reduction in the viral load of HBV was statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests. Results: No significant antiviral efficacy was noted following the application of Ashima-shi 2/100 sodium hypochlorite disinfectant. Paknaz showed the highest efficacy against HBV. Vitex and Active ranked next with significant differences (P<0.0001) Conclusion: Under the study limitations, Paknaz 2/100 sodium hypochlorite solution was the most effective while Ashimashi 2/100 sodium hypochlorite disinfectant did not show adequate efficacy against HBV.http://jidai.ir/browse.php?a_code=A-10-1-821&slc_lang=en&sid=1Hepatitis BDisinfectionSurfaceDentistry |
| spellingShingle | Mohammad reza Aghasadeghi Sakine Arami Masomeh Hasani Tabatabaie Hojjat Darvishpour Mohammad javad Kharazifard Hamid Sarlak Efficacy of Four Disinfectants Against Hepatitis B Virus Journal of Islamic Dental Association of Iran Hepatitis B Disinfection Surface Dentistry |
| title | Efficacy of Four Disinfectants Against Hepatitis B Virus |
| title_full | Efficacy of Four Disinfectants Against Hepatitis B Virus |
| title_fullStr | Efficacy of Four Disinfectants Against Hepatitis B Virus |
| title_full_unstemmed | Efficacy of Four Disinfectants Against Hepatitis B Virus |
| title_short | Efficacy of Four Disinfectants Against Hepatitis B Virus |
| title_sort | efficacy of four disinfectants against hepatitis b virus |
| topic | Hepatitis B Disinfection Surface Dentistry |
| url | http://jidai.ir/browse.php?a_code=A-10-1-821&slc_lang=en&sid=1 |
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