clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean
A random insertional mutagenesis in Colletotrichum lindemuthianum, the causal agent of common bean anthracnose, generated four mutants that showed altered pathogenicity when tested on intact seedlings, excised leaves, and/or excised hypocotyls. One of these mutants, H290, produced very few lesions o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1998-02-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.2.99 |
| _version_ | 1811264608519847936 |
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| author | Marie Dufresne John A. Bailey Michel Dron Thierry Langin |
| author_facet | Marie Dufresne John A. Bailey Michel Dron Thierry Langin |
| author_sort | Marie Dufresne |
| collection | DOAJ |
| description | A random insertional mutagenesis in Colletotrichum lindemuthianum, the causal agent of common bean anthracnose, generated four mutants that showed altered pathogenicity when tested on intact seedlings, excised leaves, and/or excised hypocotyls. One of these mutants, H290, produced very few lesions on bean leaves and appeared affected in its ability to penetrate the leaf cuticle. Molecular analyses showed that the border sequences of the unique integration site of the disrupting pAN7-1 plasmid in the mutant exhibited homology with conserved domains of serine/threonine protein kinases. The corresponding wild-type sequences were cloned and a gene replacement vector with a mutated copy harboring a selection marker constructed. Transformation of the wild-type pathogen produced a strain with a phenotype identical to the original mutant. Genomic and cDNA sequences indicated that the disrupted gene is a member of the serine/threonine protein kinase family. The gene, called clk1 (Colletotrichum lindemuthianum kinase 1), was weakly expressed in the mycelium of the wild-type strain grown on rich and minimal synthetic media but was undetectable during the infection even when a sensitive reverse transcriptase-polymerase chain reaction methodology was used. This study represents the first characterization of altered pathogenicity mutants in C. lindemuthianum produced by random mutagenesis and demonstrates the involvement of a member of the serine/threonine kinase gene family in the early steps of the infection process. |
| first_indexed | 2024-04-12T20:06:16Z |
| format | Article |
| id | doaj.art-e161d47bf4da4708a0b0ab35a1019619 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:16Z |
| publishDate | 1998-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-e161d47bf4da4708a0b0ab35a10196192022-12-22T03:18:23ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-02-011129910810.1094/MPMI.1998.11.2.99clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common BeanMarie DufresneJohn A. BaileyMichel DronThierry LanginA random insertional mutagenesis in Colletotrichum lindemuthianum, the causal agent of common bean anthracnose, generated four mutants that showed altered pathogenicity when tested on intact seedlings, excised leaves, and/or excised hypocotyls. One of these mutants, H290, produced very few lesions on bean leaves and appeared affected in its ability to penetrate the leaf cuticle. Molecular analyses showed that the border sequences of the unique integration site of the disrupting pAN7-1 plasmid in the mutant exhibited homology with conserved domains of serine/threonine protein kinases. The corresponding wild-type sequences were cloned and a gene replacement vector with a mutated copy harboring a selection marker constructed. Transformation of the wild-type pathogen produced a strain with a phenotype identical to the original mutant. Genomic and cDNA sequences indicated that the disrupted gene is a member of the serine/threonine protein kinase family. The gene, called clk1 (Colletotrichum lindemuthianum kinase 1), was weakly expressed in the mycelium of the wild-type strain grown on rich and minimal synthetic media but was undetectable during the infection even when a sensitive reverse transcriptase-polymerase chain reaction methodology was used. This study represents the first characterization of altered pathogenicity mutants in C. lindemuthianum produced by random mutagenesis and demonstrates the involvement of a member of the serine/threonine kinase gene family in the early steps of the infection process.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.2.99 |
| spellingShingle | Marie Dufresne John A. Bailey Michel Dron Thierry Langin clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean Molecular Plant-Microbe Interactions |
| title | clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean |
| title_full | clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean |
| title_fullStr | clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean |
| title_full_unstemmed | clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean |
| title_short | clk1, a Serine/Threonine Protein Kinase-Encoding Gene, Is Involved in Pathogenicity of Colletotrichum lindemuthianum on Common Bean |
| title_sort | clk1 a serine threonine protein kinase encoding gene is involved in pathogenicity of colletotrichum lindemuthianum on common bean |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.2.99 |
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