Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost.
The study aimed to characterize hydro-methanolic (25%) extracts of Justicia adhatoda L. (stem and flower) and Cordia dichotoma Frost. (Stem and fruit) and evaluate the in silico thrombolytic properties of the major phytocompounds present in the plants. In the preliminary fluorescence imaging throug...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2023-09-01
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| Series: | Notulae Scientia Biologicae |
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| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11625 |
| _version_ | 1797670749958307840 |
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| author | Annika Maria PAUL Anish NAG |
| author_facet | Annika Maria PAUL Anish NAG |
| author_sort | Annika Maria PAUL |
| collection | DOAJ |
| description |
The study aimed to characterize hydro-methanolic (25%) extracts of Justicia adhatoda L. (stem and flower) and Cordia dichotoma Frost. (Stem and fruit) and evaluate the in silico thrombolytic properties of the major phytocompounds present in the plants. In the preliminary fluorescence imaging through treatment by different reagents, both plants were found to be pharmacologically active. Further qualitative screening of phytochemicals, spectroscopy-based techniques, namely, UV-Vis Spectroscopy and FTIR, revealed various classes of compounds such as polyacetylenes, aglycones, quercetin, anthocyanins, anthraquinones, alkaloids, chalcones and aurones, flavanols, carotenoids, and flavanones. Further, by the application of Thin Layer Chromatography, phenols and flavonoids, namely Catechol, Kaempferol, Quercetin, and Lutein, along with other compounds like Chlorophyll b, Glutamic Acid, and Tryptophan were identified from the extracts. Finally, in the molecular docking study, three compounds, Datiscoside and Robinin of C. dichotoma and Daucosterol of J. adhatoda showed high binding energies (-10.224, -9.547 and -9.262 kcal mol-1 respectively) towards the G-protein coupled thrombotic platelet aggregation receptor P2Y1 when compared to that of the control MRS2500 (-7.148 kcal mol-1).
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| first_indexed | 2024-03-11T21:05:03Z |
| format | Article |
| id | doaj.art-e1625a55b852452cb19cdd9997827e13 |
| institution | Directory Open Access Journal |
| issn | 2067-3264 |
| language | English |
| last_indexed | 2024-03-11T21:05:03Z |
| publishDate | 2023-09-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-e1625a55b852452cb19cdd9997827e132023-09-29T14:35:58ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32642023-09-0115310.55779/nsb15311625Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost.Annika Maria PAUL0Anish NAG1CHRIST (Deemed to be University), Department of Life Sciences, Bangalore 560029, KarnatakaCHRIST (Deemed to be University), Department of Life Sciences, Bangalore 560029, Karnataka The study aimed to characterize hydro-methanolic (25%) extracts of Justicia adhatoda L. (stem and flower) and Cordia dichotoma Frost. (Stem and fruit) and evaluate the in silico thrombolytic properties of the major phytocompounds present in the plants. In the preliminary fluorescence imaging through treatment by different reagents, both plants were found to be pharmacologically active. Further qualitative screening of phytochemicals, spectroscopy-based techniques, namely, UV-Vis Spectroscopy and FTIR, revealed various classes of compounds such as polyacetylenes, aglycones, quercetin, anthocyanins, anthraquinones, alkaloids, chalcones and aurones, flavanols, carotenoids, and flavanones. Further, by the application of Thin Layer Chromatography, phenols and flavonoids, namely Catechol, Kaempferol, Quercetin, and Lutein, along with other compounds like Chlorophyll b, Glutamic Acid, and Tryptophan were identified from the extracts. Finally, in the molecular docking study, three compounds, Datiscoside and Robinin of C. dichotoma and Daucosterol of J. adhatoda showed high binding energies (-10.224, -9.547 and -9.262 kcal mol-1 respectively) towards the G-protein coupled thrombotic platelet aggregation receptor P2Y1 when compared to that of the control MRS2500 (-7.148 kcal mol-1). https://www.notulaebiologicae.ro/index.php/nsb/article/view/11625analytical characterizationmolecular dockingphytochemicalsthrombosis |
| spellingShingle | Annika Maria PAUL Anish NAG Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost. Notulae Scientia Biologicae analytical characterization molecular docking phytochemicals thrombosis |
| title | Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost. |
| title_full | Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost. |
| title_fullStr | Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost. |
| title_full_unstemmed | Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost. |
| title_short | Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost. |
| title_sort | phytochemical fingerprinting and evaluation of in silico anti thrombotic properties of justicia adhatoda l and cordia dichotoma frost |
| topic | analytical characterization molecular docking phytochemicals thrombosis |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11625 |
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