EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS
In this study, we surveyed the epigenetic variations in salt stressed Opuntias belonging to four Algerian species; Opuntia species: O. ficus indica, O. amycleae, O. streptacantha, and O. engelmannii. Plants response to salt stress (0, 200 mM, 400 mM and 600 mM concentrations of Na Cl) was evaluated...
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| Format: | Article |
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Editura Universităţii din Oradea
2021-05-01
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| Series: | Natural Resources and Sustainable Development |
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| Online Access: | https://docs.google.com/viewerng/viewer?url=http://nrsdj.com/papers/2021-1/07.+Hadj+Kouider+Boubakr.pdf |
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| author | Hadj Kouider Boubakr Lallouche Bahia Ammar Boutekrabt Ben Romdhane Mériam Zoghlami Néjia |
| author_facet | Hadj Kouider Boubakr Lallouche Bahia Ammar Boutekrabt Ben Romdhane Mériam Zoghlami Néjia |
| author_sort | Hadj Kouider Boubakr |
| collection | DOAJ |
| description | In this study, we surveyed the epigenetic variations in salt stressed Opuntias belonging to four
Algerian species; Opuntia species: O. ficus indica, O. amycleae, O. streptacantha, and O. engelmannii. Plants response to salt stress (0, 200 mM, 400 mM and 600 mM concentrations of Na Cl) was evaluated. Then, RAPD markers were used to apprehend the epigenetic variations at the DNA level. The analyses of the epigenetic variations were conducted using the random primers UBC228, UBC231 and UBC241 that generated 57 polymorphic markers. Results have shown that in comparison to the control plants, 13 markers were lacking in the control but induced by salt stress application. On the other hand, 02 markers were only present in the control plants. Interestingly, some markers were only induced at the highest salinity concentration (600 mM) and two bands were specific of the tolerant species O. engelmannii. In all, the 15 detected specific markers may be strongly involved in Marker-Assisted Selection studies against salt stress in cactus. Therefore, DNA sequencing of these interesting private markers may aid the identification of putative salt resistance genes, and genetic transformation procedures will subsequently facilitate their introgression in Opuntias to cope soil salinization. |
| first_indexed | 2024-12-14T20:46:49Z |
| format | Article |
| id | doaj.art-e16784a3aded4587b049ea3e8e225d67 |
| institution | Directory Open Access Journal |
| issn | 2066-6276 2601-5676 |
| language | English |
| last_indexed | 2024-12-14T20:46:49Z |
| publishDate | 2021-05-01 |
| publisher | Editura Universităţii din Oradea |
| record_format | Article |
| series | Natural Resources and Sustainable Development |
| spelling | doaj.art-e16784a3aded4587b049ea3e8e225d672022-12-21T22:48:01ZengEditura Universităţii din OradeaNatural Resources and Sustainable Development2066-62762601-56762021-05-01111667610.31924/nrsd.v11i1.067EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESSHadj Kouider Boubakr0Lallouche Bahia1Ammar Boutekrabt2Ben Romdhane Mériam3Zoghlami Néjia4Mohamed Boudiaf University - M’sila, Faculty of Sciences, Department of Agricultural Sciences, Laboratory of Biodiversity and Biotechnological Techniques of Plant Resource Development, AlgeriaMohamed Boudiaf University - M’sila, Faculty of Sciences, Department of Agricultural Sciences, Laboratory of Biodiversity and Biotechnological Techniques of Plant Resource Development, AlgeriaSaad Dahlab University, Blida, Faculty of Nature and Life Sciences, Department of Biotechnology, AlgeriaBiotechnology Centre of Borj-Cédria, Laboratory of Plant Molecular Physiology, Hammam-lif, TunisiaBiotechnology Centre of Borj-Cédria, Laboratory of Plant Molecular Physiology, Hammam-lif, TunisiaIn this study, we surveyed the epigenetic variations in salt stressed Opuntias belonging to four Algerian species; Opuntia species: O. ficus indica, O. amycleae, O. streptacantha, and O. engelmannii. Plants response to salt stress (0, 200 mM, 400 mM and 600 mM concentrations of Na Cl) was evaluated. Then, RAPD markers were used to apprehend the epigenetic variations at the DNA level. The analyses of the epigenetic variations were conducted using the random primers UBC228, UBC231 and UBC241 that generated 57 polymorphic markers. Results have shown that in comparison to the control plants, 13 markers were lacking in the control but induced by salt stress application. On the other hand, 02 markers were only present in the control plants. Interestingly, some markers were only induced at the highest salinity concentration (600 mM) and two bands were specific of the tolerant species O. engelmannii. In all, the 15 detected specific markers may be strongly involved in Marker-Assisted Selection studies against salt stress in cactus. Therefore, DNA sequencing of these interesting private markers may aid the identification of putative salt resistance genes, and genetic transformation procedures will subsequently facilitate their introgression in Opuntias to cope soil salinization.https://docs.google.com/viewerng/viewer?url=http://nrsdj.com/papers/2021-1/07.+Hadj+Kouider+Boubakr.pdfopuntia; epigenetic variability; rapd markers; salt stress |
| spellingShingle | Hadj Kouider Boubakr Lallouche Bahia Ammar Boutekrabt Ben Romdhane Mériam Zoghlami Néjia EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS Natural Resources and Sustainable Development opuntia; epigenetic variability; rapd markers; salt stress |
| title | EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS |
| title_full | EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS |
| title_fullStr | EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS |
| title_full_unstemmed | EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS |
| title_short | EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS |
| title_sort | epigenetic evaluation in algerian opuntia species under salt stress |
| topic | opuntia; epigenetic variability; rapd markers; salt stress |
| url | https://docs.google.com/viewerng/viewer?url=http://nrsdj.com/papers/2021-1/07.+Hadj+Kouider+Boubakr.pdf |
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