Chemical basis of microbiome preference in the nematode C. elegans
Abstract Animals are exposed to many microbes in their environment, some of which have been shown to colonize various tissues including the intestine. The composition of the intestinal microbiota affects many aspects of the host’s physiology and health. Despite this, very little is known about wheth...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51533-6 |
| _version_ | 1797349958846775296 |
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| author | Victor Z. Chai Tiam Farajzadeh Yufei Meng Sokhna B. Lo Tymmaa A. Asaed Charles J. Taylor Elizabeth E. Glater |
| author_facet | Victor Z. Chai Tiam Farajzadeh Yufei Meng Sokhna B. Lo Tymmaa A. Asaed Charles J. Taylor Elizabeth E. Glater |
| author_sort | Victor Z. Chai |
| collection | DOAJ |
| description | Abstract Animals are exposed to many microbes in their environment, some of which have been shown to colonize various tissues including the intestine. The composition of the intestinal microbiota affects many aspects of the host’s physiology and health. Despite this, very little is known about whether host behavior contributes to the colonization. We approach this question in the nematode C. elegans, which feeds on bacteria and also harbors an intestinal microbiome. We examined the behavior of C. elegans towards CeMbio, a simplified microbiome consisting of twelve strains that represent the bacteria found in the animal’s natural environment. We observed that C. elegans raised on E. coli shows a strong preference for three members of CeMbio (Lelliottia amnigena JUb66, Enterobacter hormaechei CEent1, and Pantoea nemavictus BIGb0393) compared to E. coli. Previously, these three bacterial strains have been shown to support faster C. elegans development time than E. coli OP50 and are low colonizers compared to eight other members of CeMbio. We then used gas chromatography coupled to mass spectrometry to identify that these three bacteria release isoamyl alcohol, a previously described C. elegans chemoattractant. We suggest that C. elegans seeks bacteria that release isoamyl alcohol and support faster growth. |
| first_indexed | 2024-03-08T12:37:53Z |
| format | Article |
| id | doaj.art-e1679926304c49e39819359c5ee2680d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:37:53Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e1679926304c49e39819359c5ee2680d2024-01-21T12:21:52ZengNature PortfolioScientific Reports2045-23222024-01-011411810.1038/s41598-024-51533-6Chemical basis of microbiome preference in the nematode C. elegansVictor Z. Chai0Tiam Farajzadeh1Yufei Meng2Sokhna B. Lo3Tymmaa A. Asaed4Charles J. Taylor5Elizabeth E. Glater6Department of Neuroscience, Pomona CollegeDepartment of Chemistry, Pomona CollegeDepartment of Neuroscience, Pomona CollegeDepartment of Neuroscience, Pomona CollegeDepartment of Neuroscience, Pomona CollegeDepartment of Chemistry, Pomona CollegeDepartment of Neuroscience, Pomona CollegeAbstract Animals are exposed to many microbes in their environment, some of which have been shown to colonize various tissues including the intestine. The composition of the intestinal microbiota affects many aspects of the host’s physiology and health. Despite this, very little is known about whether host behavior contributes to the colonization. We approach this question in the nematode C. elegans, which feeds on bacteria and also harbors an intestinal microbiome. We examined the behavior of C. elegans towards CeMbio, a simplified microbiome consisting of twelve strains that represent the bacteria found in the animal’s natural environment. We observed that C. elegans raised on E. coli shows a strong preference for three members of CeMbio (Lelliottia amnigena JUb66, Enterobacter hormaechei CEent1, and Pantoea nemavictus BIGb0393) compared to E. coli. Previously, these three bacterial strains have been shown to support faster C. elegans development time than E. coli OP50 and are low colonizers compared to eight other members of CeMbio. We then used gas chromatography coupled to mass spectrometry to identify that these three bacteria release isoamyl alcohol, a previously described C. elegans chemoattractant. We suggest that C. elegans seeks bacteria that release isoamyl alcohol and support faster growth.https://doi.org/10.1038/s41598-024-51533-6 |
| spellingShingle | Victor Z. Chai Tiam Farajzadeh Yufei Meng Sokhna B. Lo Tymmaa A. Asaed Charles J. Taylor Elizabeth E. Glater Chemical basis of microbiome preference in the nematode C. elegans Scientific Reports |
| title | Chemical basis of microbiome preference in the nematode C. elegans |
| title_full | Chemical basis of microbiome preference in the nematode C. elegans |
| title_fullStr | Chemical basis of microbiome preference in the nematode C. elegans |
| title_full_unstemmed | Chemical basis of microbiome preference in the nematode C. elegans |
| title_short | Chemical basis of microbiome preference in the nematode C. elegans |
| title_sort | chemical basis of microbiome preference in the nematode c elegans |
| url | https://doi.org/10.1038/s41598-024-51533-6 |
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