Organic fluorescent probes for live-cell super-resolution imaging
Abstract The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Springer & Higher Education Press
2023-11-01
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| Series: | Frontiers of Optoelectronics |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s12200-023-00090-3 |
| _version_ | 1797630493810753536 |
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| author | Xinxin Duan Meng Zhang Yu-Hui Zhang |
| author_facet | Xinxin Duan Meng Zhang Yu-Hui Zhang |
| author_sort | Xinxin Duan |
| collection | DOAJ |
| description | Abstract The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both advanced imaging systems and excellent fluorescent probes are required. Traditional fluorescent probes have good availability, but that is not the case for probes for live-cell super-resolution imaging. In this review, we first introduce the principles of various super-resolution technologies and their probe requirements, then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging, and finally provide a brief conclusion and overview of the future. Graphical Abstract |
| first_indexed | 2024-03-11T11:07:55Z |
| format | Article |
| id | doaj.art-e16b262781ba4b6f9b3c526e50c6246e |
| institution | Directory Open Access Journal |
| issn | 2095-2767 |
| language | English |
| last_indexed | 2024-03-11T11:07:55Z |
| publishDate | 2023-11-01 |
| publisher | Springer & Higher Education Press |
| record_format | Article |
| series | Frontiers of Optoelectronics |
| spelling | doaj.art-e16b262781ba4b6f9b3c526e50c6246e2023-11-12T12:06:47ZengSpringer & Higher Education PressFrontiers of Optoelectronics2095-27672023-11-0116111210.1007/s12200-023-00090-3Organic fluorescent probes for live-cell super-resolution imagingXinxin Duan0Meng Zhang1Yu-Hui Zhang2Britton Chance Center for Biomedical Photonics, MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics, MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and TechnologyAbstract The development of super-resolution technology has made it possible to investigate the ultrastructure of intracellular organelles by fluorescence microscopy, which has greatly facilitated the development of life sciences and biomedicine. To realize super-resolution imaging of living cells, both advanced imaging systems and excellent fluorescent probes are required. Traditional fluorescent probes have good availability, but that is not the case for probes for live-cell super-resolution imaging. In this review, we first introduce the principles of various super-resolution technologies and their probe requirements, then summarize the existing designs and delivery strategies of super-resolution probes for live-cell imaging, and finally provide a brief conclusion and overview of the future. Graphical Abstracthttps://doi.org/10.1007/s12200-023-00090-3Super-resolution imagingOrganic fluorescent dyesLive-cell imagingCell-impermeable organic probes |
| spellingShingle | Xinxin Duan Meng Zhang Yu-Hui Zhang Organic fluorescent probes for live-cell super-resolution imaging Frontiers of Optoelectronics Super-resolution imaging Organic fluorescent dyes Live-cell imaging Cell-impermeable organic probes |
| title | Organic fluorescent probes for live-cell super-resolution imaging |
| title_full | Organic fluorescent probes for live-cell super-resolution imaging |
| title_fullStr | Organic fluorescent probes for live-cell super-resolution imaging |
| title_full_unstemmed | Organic fluorescent probes for live-cell super-resolution imaging |
| title_short | Organic fluorescent probes for live-cell super-resolution imaging |
| title_sort | organic fluorescent probes for live cell super resolution imaging |
| topic | Super-resolution imaging Organic fluorescent dyes Live-cell imaging Cell-impermeable organic probes |
| url | https://doi.org/10.1007/s12200-023-00090-3 |
| work_keys_str_mv | AT xinxinduan organicfluorescentprobesforlivecellsuperresolutionimaging AT mengzhang organicfluorescentprobesforlivecellsuperresolutionimaging AT yuhuizhang organicfluorescentprobesforlivecellsuperresolutionimaging |