Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing
The aim of this study was to fabricate and characterize various concentrations of peppermint essential oil (PEP) loaded on poly(ε-caprolactone) (PCL) electrospun fiber mats for healing applications, where PEP was intended to impart antibacterial activity to the fibers. SEM images illustrated that th...
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| Format: | Article |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00346/full |
| _version_ | 1819117037189857280 |
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| author | Irem Unalan Benedikt Slavik Andrea Buettner Wolfgang H. Goldmann Gerhard Frank Aldo R. Boccaccini |
| author_facet | Irem Unalan Benedikt Slavik Andrea Buettner Wolfgang H. Goldmann Gerhard Frank Aldo R. Boccaccini |
| author_sort | Irem Unalan |
| collection | DOAJ |
| description | The aim of this study was to fabricate and characterize various concentrations of peppermint essential oil (PEP) loaded on poly(ε-caprolactone) (PCL) electrospun fiber mats for healing applications, where PEP was intended to impart antibacterial activity to the fibers. SEM images illustrated that the morphology of all electrospun fiber mats was smooth, uniform, and bead-free. The average fiber diameter was reduced by the addition of PEP from 1.6 ± 0.1 to 1.0 ± 0.2 μm. Functional groups of the fibers were determined by Raman spectroscopy. Gas chromatography-mass spectroscopy (GC-MS) analysis demonstrated the actual PEP content in the samples. In vitro degradation was determined by measuring weight loss and their morphology change, showing that the electrospun fibers slightly degraded by the addition of PEP. The wettability of PCL and PEP loaded electrospun fiber mats was measured by determining contact angle and it was shown that wettability increased with the incorporation of PEP. The antimicrobial activity results revealed that PEP loaded PCL electrospun fiber mats exhibited inhibition against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. In addition, an in-vitro cell viability assay using normal human dermal fibroblast (NHDF) cells revealed improved cell viability on PCL, PCLPEP1.5, PCLPEP3, and PCLGEL6 electrospun fiber mats compared to the control (CNT) after 48 h cell culture. Our findings showed for the first time PEP loaded PCL electrospun fiber mats with antibiotic-free antibacterial activity as promising candidates for wound healing applications. |
| first_indexed | 2024-12-22T05:26:36Z |
| format | Article |
| id | doaj.art-e16bf0af615b42babb3dd7df907f2d75 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T05:26:36Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-e16bf0af615b42babb3dd7df907f2d752022-12-21T18:37:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-11-01710.3389/fbioe.2019.00346488792Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound HealingIrem Unalan0Benedikt Slavik1Andrea Buettner2Wolfgang H. Goldmann3Gerhard Frank4Aldo R. Boccaccini5Department of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyChair of Aroma and Smell Research, Department of Chemistry and Pharmacy, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyChair of Aroma and Smell Research, Department of Chemistry and Pharmacy, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyDepartment of Physics, Institute of Biophysics, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, GermanyThe aim of this study was to fabricate and characterize various concentrations of peppermint essential oil (PEP) loaded on poly(ε-caprolactone) (PCL) electrospun fiber mats for healing applications, where PEP was intended to impart antibacterial activity to the fibers. SEM images illustrated that the morphology of all electrospun fiber mats was smooth, uniform, and bead-free. The average fiber diameter was reduced by the addition of PEP from 1.6 ± 0.1 to 1.0 ± 0.2 μm. Functional groups of the fibers were determined by Raman spectroscopy. Gas chromatography-mass spectroscopy (GC-MS) analysis demonstrated the actual PEP content in the samples. In vitro degradation was determined by measuring weight loss and their morphology change, showing that the electrospun fibers slightly degraded by the addition of PEP. The wettability of PCL and PEP loaded electrospun fiber mats was measured by determining contact angle and it was shown that wettability increased with the incorporation of PEP. The antimicrobial activity results revealed that PEP loaded PCL electrospun fiber mats exhibited inhibition against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. In addition, an in-vitro cell viability assay using normal human dermal fibroblast (NHDF) cells revealed improved cell viability on PCL, PCLPEP1.5, PCLPEP3, and PCLGEL6 electrospun fiber mats compared to the control (CNT) after 48 h cell culture. Our findings showed for the first time PEP loaded PCL electrospun fiber mats with antibiotic-free antibacterial activity as promising candidates for wound healing applications.https://www.frontiersin.org/article/10.3389/fbioe.2019.00346/fullpeppermint essential oilpoly (ε-caprolactone)electrospinningantibacterial activitywound healing |
| spellingShingle | Irem Unalan Benedikt Slavik Andrea Buettner Wolfgang H. Goldmann Gerhard Frank Aldo R. Boccaccini Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing Frontiers in Bioengineering and Biotechnology peppermint essential oil poly (ε-caprolactone) electrospinning antibacterial activity wound healing |
| title | Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing |
| title_full | Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing |
| title_fullStr | Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing |
| title_full_unstemmed | Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing |
| title_short | Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing |
| title_sort | physical and antibacterial properties of peppermint essential oil loaded poly ε caprolactone pcl electrospun fiber mats for wound healing |
| topic | peppermint essential oil poly (ε-caprolactone) electrospinning antibacterial activity wound healing |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00346/full |
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