Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle
Background and Aim: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-01-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.16/January-2023/20.pdf |
| _version_ | 1811176983904649216 |
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| author | Indira Saltanovna Beishova Alena Valentinovna Belaya Yusupzhan Artykovich Yuldashbayev Gulzhagan Dzhambulovna Chuzhebayeva Vadim Alexandrovich Ulyanov Tatyana Vladimirovna Ulyanova Alexandr Mikhailovich Kovalchuk Ulbolsyn Zhangaziyevna Kuzhebayeva Aidar Myrzahmetuly Namet |
| author_facet | Indira Saltanovna Beishova Alena Valentinovna Belaya Yusupzhan Artykovich Yuldashbayev Gulzhagan Dzhambulovna Chuzhebayeva Vadim Alexandrovich Ulyanov Tatyana Vladimirovna Ulyanova Alexandr Mikhailovich Kovalchuk Ulbolsyn Zhangaziyevna Kuzhebayeva Aidar Myrzahmetuly Namet |
| author_sort | Indira Saltanovna Beishova |
| collection | DOAJ |
| description | Background and Aim: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize infectious disease risks. To this end, the most relevant study field is the search for gene sites that impact production and health. This study aimed to determine the nature of the distribution of the relative frequencies of alleles and genotypes of polymorphic prolactin (PRL) and nitric oxide synthase (NOS2) in Holstein cows and identify the relationship of these genes with resistance to mastitis and bovine leukemia.
Materials and Methods: For this study, we chose cows because infectious diseases affect the amount of lactation and milk quality. Holstein cattle with mastitis and bovine leukemia were selected. Animal genotypes were determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. The results were analyzed using a nonparametric statistical method using Microsoft Excel 2010 and Statistica 6.0.
Results: In healthy animals, 94 genotypes were identified for both genes under study. For bPRL, bPRL-RsaIAA (72) was the most common genotype and bPRL-RsaIBB (4) the least; for NOS2, bNOS2-HinfIAB (47) was the most common genotype and bNOS2-HinfIAA the least (21). In animals with leukemia, 34 genotypes were identified. For PRL, bPRL-RsaIAA (25) was the most common genotype and bPRL-RsaIBB (2) the least; for NOS2, bNOS2-HinfIBB (17) was the most common genotype and bNOS2-HinfIAA (3) the least. In animals with mastitis, 67 genotypes were identified. For PRL, bPRL-RsaIAA (43) was the most common genotype and bPRL-RsaIBB (6) the least; for NOS2, bNOS2-HinfIBB (31) was the most common genotype and bNOS2-HinfIAA (7) the least. The distribution of genotypes of polymorphic bPRL and bNOS2 generally coincides, and bPRL-RsaIBB is the most common genotype. In groups of sick animals, the number of bNOS2-HinfIAA homozygotes was lower than that of the control group. In particular, the proportion of animals with the bNOS2-HinfIAA genotype with bovine leukemia was 8.7% and with mastitis was 10.3% compared with 22.4% in healthy animals. These data support the possible association of the bNOS2-HinfIAA genotype with resistance to infection. The frequency of the bPRL-RsaIB allele was higher in groups of sick animals. This allele is associated with increased milk productivity, suggesting that highly productive animals are less resistant to the incidence of viral bovine leukemia and mastitis of bacterial etiology.
Conclusion: DNA amplification of Holstein cattle for the polymorphic regions of PRL and NOS2 using the PCR-RFLP method revealed a possible connection between the distribution of relative allele frequencies of bPRL and bNOS2 and resistance to viral and bacterial infections. Thus, in groups of sick animals, the frequency of bPRL-RsaIBB, associated with increased milk production compared with the theoretically calculated equilibrium value was higher and the number of homozygotes bNOS2-HinfIAA was lower than in the control group. In conclusion, animals with increased milk production were more prone to diseases, such as mastitis and bovine leukemia. |
| first_indexed | 2024-04-10T20:00:52Z |
| format | Article |
| id | doaj.art-e16d0e98cb7e439aad5a57ad5a12dc40 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-10T20:00:52Z |
| publishDate | 2023-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-e16d0e98cb7e439aad5a57ad5a12dc402023-01-27T07:07:40ZengVeterinary WorldVeterinary World0972-89882231-09162023-01-0116116116710.14202/vetworld.2023.161-167Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattleIndira Saltanovna Beishova0https://orcid.org/0000-0001-5293-2190Alena Valentinovna Belaya1https://orcid.org/0000-0003-1786-0341Yusupzhan Artykovich Yuldashbayev2https://orcid.org/0000-0002-7150-1131Gulzhagan Dzhambulovna Chuzhebayeva3https://orcid.org/0000-0002-0091-8888Vadim Alexandrovich Ulyanov4https://orcid.org/0000-0002-7500-1601Tatyana Vladimirovna Ulyanova5https://orcid.org/0000-0002-4814-2601Alexandr Mikhailovich Kovalchuk6https://orcid.org/0000-0002-4106-4954Ulbolsyn Zhangaziyevna Kuzhebayeva7https://orcid.org/0000-0002-7887-3376Aidar Myrzahmetuly Namet8https://orcid.org/0000-0001-9639-4208Non-profit JSC "Zhangir Khan West Kazakhstan Agrarian Technical University", Uralsk, Republic of Kazakhstan.Belarusian State Pedagogical University named after Maxim Tank, Minsk, Republic of Belarus.Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russian Federation.Non-profit JSC "A. Baitursynov Kostanay Regional University", Kostanay, Republic of Kazakhstan.Non-profit JSC "Zhangir Khan West Kazakhstan Agrarian Technical University", Uralsk, Republic of Kazakhstan.Non-profit JSC "Zhangir Khan West Kazakhstan Agrarian Technical University", Uralsk, Republic of Kazakhstan.Non-profit JSC "Zhangir Khan West Kazakhstan Agrarian Technical University", Uralsk, Republic of Kazakhstan.Non-profit JSC "Zhangir Khan West Kazakhstan Agrarian Technical University", Uralsk, Republic of Kazakhstan.MVA Group "Scientific-Research Production Center" Ltd., Almaty, Republic of Kazakhstan.Background and Aim: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize infectious disease risks. To this end, the most relevant study field is the search for gene sites that impact production and health. This study aimed to determine the nature of the distribution of the relative frequencies of alleles and genotypes of polymorphic prolactin (PRL) and nitric oxide synthase (NOS2) in Holstein cows and identify the relationship of these genes with resistance to mastitis and bovine leukemia. Materials and Methods: For this study, we chose cows because infectious diseases affect the amount of lactation and milk quality. Holstein cattle with mastitis and bovine leukemia were selected. Animal genotypes were determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. The results were analyzed using a nonparametric statistical method using Microsoft Excel 2010 and Statistica 6.0. Results: In healthy animals, 94 genotypes were identified for both genes under study. For bPRL, bPRL-RsaIAA (72) was the most common genotype and bPRL-RsaIBB (4) the least; for NOS2, bNOS2-HinfIAB (47) was the most common genotype and bNOS2-HinfIAA the least (21). In animals with leukemia, 34 genotypes were identified. For PRL, bPRL-RsaIAA (25) was the most common genotype and bPRL-RsaIBB (2) the least; for NOS2, bNOS2-HinfIBB (17) was the most common genotype and bNOS2-HinfIAA (3) the least. In animals with mastitis, 67 genotypes were identified. For PRL, bPRL-RsaIAA (43) was the most common genotype and bPRL-RsaIBB (6) the least; for NOS2, bNOS2-HinfIBB (31) was the most common genotype and bNOS2-HinfIAA (7) the least. The distribution of genotypes of polymorphic bPRL and bNOS2 generally coincides, and bPRL-RsaIBB is the most common genotype. In groups of sick animals, the number of bNOS2-HinfIAA homozygotes was lower than that of the control group. In particular, the proportion of animals with the bNOS2-HinfIAA genotype with bovine leukemia was 8.7% and with mastitis was 10.3% compared with 22.4% in healthy animals. These data support the possible association of the bNOS2-HinfIAA genotype with resistance to infection. The frequency of the bPRL-RsaIB allele was higher in groups of sick animals. This allele is associated with increased milk productivity, suggesting that highly productive animals are less resistant to the incidence of viral bovine leukemia and mastitis of bacterial etiology. Conclusion: DNA amplification of Holstein cattle for the polymorphic regions of PRL and NOS2 using the PCR-RFLP method revealed a possible connection between the distribution of relative allele frequencies of bPRL and bNOS2 and resistance to viral and bacterial infections. Thus, in groups of sick animals, the frequency of bPRL-RsaIBB, associated with increased milk production compared with the theoretically calculated equilibrium value was higher and the number of homozygotes bNOS2-HinfIAA was lower than in the control group. In conclusion, animals with increased milk production were more prone to diseases, such as mastitis and bovine leukemia.http://www.veterinaryworld.org/Vol.16/January-2023/20.pdfbovine leukemiacattleholstein breedmastitispolymerase chain reaction-restriction fragment length polymorphismpolymorphism |
| spellingShingle | Indira Saltanovna Beishova Alena Valentinovna Belaya Yusupzhan Artykovich Yuldashbayev Gulzhagan Dzhambulovna Chuzhebayeva Vadim Alexandrovich Ulyanov Tatyana Vladimirovna Ulyanova Alexandr Mikhailovich Kovalchuk Ulbolsyn Zhangaziyevna Kuzhebayeva Aidar Myrzahmetuly Namet Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle Veterinary World bovine leukemia cattle holstein breed mastitis polymerase chain reaction-restriction fragment length polymorphism polymorphism |
| title | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
| title_full | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
| title_fullStr | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
| title_full_unstemmed | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
| title_short | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
| title_sort | genetic polymorphism of prolactin and nitric oxide synthase in holstein cattle |
| topic | bovine leukemia cattle holstein breed mastitis polymerase chain reaction-restriction fragment length polymorphism polymorphism |
| url | http://www.veterinaryworld.org/Vol.16/January-2023/20.pdf |
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