Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL?
The manufacturer's instructions for the venereal disease research laboratory (VDRL) antigen test for diagnosing neurosyphilis describe testing of serum samples and do not include procedures for cerebrospinal fluid (CSF) testing. This study compared the CSF-VDRL test with 10 μL of antigen (CSF-V...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.877186/full |
| _version_ | 1811303959637262336 |
|---|---|
| author | Yao Xiao Yao Xiao Wei Li Wei Li Qiu-Ling Li Qiu-Ling Li Qiu-Yan Xu Qiu-Yan Xu Yang Yang Tian-Ci Yang Tian-Ci Yang Li-Li Liu Li-Li Liu Wei-Ming Gu |
| author_facet | Yao Xiao Yao Xiao Wei Li Wei Li Qiu-Ling Li Qiu-Ling Li Qiu-Yan Xu Qiu-Yan Xu Yang Yang Tian-Ci Yang Tian-Ci Yang Li-Li Liu Li-Li Liu Wei-Ming Gu |
| author_sort | Yao Xiao |
| collection | DOAJ |
| description | The manufacturer's instructions for the venereal disease research laboratory (VDRL) antigen test for diagnosing neurosyphilis describe testing of serum samples and do not include procedures for cerebrospinal fluid (CSF) testing. This study compared the CSF-VDRL test with 10 μL of antigen (CSF-VDRL-10) according to the American Public Health Association to the CSF-VDRL test with 17 μL of antigen (CSF-VDRL-17) according to the VDRL serum procedure. A total of 121 neurosyphilis patients and 86 syphilis/non-neurosyphilis patients were included. The sensitivities of the CSF-VDRL-10 and CSF-VDRL-17 tests were comparable for neurosyphilis diagnosis. The positive rate of the CSF-VDRL-17 test was higher than that of the CSF-VDRL-10 test. In all, 78.3% of the quantitative CSF-VDRL-17 results were consistent with those of the CSF-VDRL-10 test, 18.4% exhibited one-titer higher results than those of the CSF-VDRL-10 test, and 3.4% had positive CSF-VDRL-17 results but negative CSF-VDRL-10 results. The CSF-VDRL test with 17 μL of antigen was more sensitive, and it is worth performing longitudinal studies to understand its practical implications. |
| first_indexed | 2024-04-13T07:57:26Z |
| format | Article |
| id | doaj.art-e16ff67c4ba34b858a20faddcb95311d |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-13T07:57:26Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-e16ff67c4ba34b858a20faddcb95311d2022-12-22T02:55:22ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-04-01910.3389/fmed.2022.877186877186Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL?Yao Xiao0Yao Xiao1Wei Li2Wei Li3Qiu-Ling Li4Qiu-Ling Li5Qiu-Yan Xu6Qiu-Yan Xu7Yang Yang8Tian-Ci Yang9Tian-Ci Yang10Li-Li Liu11Li-Li Liu12Wei-Ming Gu13Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaDepartment of Hospital Infection Management, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaInstitute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaInstitute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaInstitute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaShanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaInstitute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaInstitute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaShanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, ChinaThe manufacturer's instructions for the venereal disease research laboratory (VDRL) antigen test for diagnosing neurosyphilis describe testing of serum samples and do not include procedures for cerebrospinal fluid (CSF) testing. This study compared the CSF-VDRL test with 10 μL of antigen (CSF-VDRL-10) according to the American Public Health Association to the CSF-VDRL test with 17 μL of antigen (CSF-VDRL-17) according to the VDRL serum procedure. A total of 121 neurosyphilis patients and 86 syphilis/non-neurosyphilis patients were included. The sensitivities of the CSF-VDRL-10 and CSF-VDRL-17 tests were comparable for neurosyphilis diagnosis. The positive rate of the CSF-VDRL-17 test was higher than that of the CSF-VDRL-10 test. In all, 78.3% of the quantitative CSF-VDRL-17 results were consistent with those of the CSF-VDRL-10 test, 18.4% exhibited one-titer higher results than those of the CSF-VDRL-10 test, and 3.4% had positive CSF-VDRL-17 results but negative CSF-VDRL-10 results. The CSF-VDRL test with 17 μL of antigen was more sensitive, and it is worth performing longitudinal studies to understand its practical implications.https://www.frontiersin.org/articles/10.3389/fmed.2022.877186/fullCSFVDRLantigen concentrationneurosyphilisdiagnosis |
| spellingShingle | Yao Xiao Yao Xiao Wei Li Wei Li Qiu-Ling Li Qiu-Ling Li Qiu-Yan Xu Qiu-Yan Xu Yang Yang Tian-Ci Yang Tian-Ci Yang Li-Li Liu Li-Li Liu Wei-Ming Gu Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL? Frontiers in Medicine CSF VDRL antigen concentration neurosyphilis diagnosis |
| title | Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL? |
| title_full | Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL? |
| title_fullStr | Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL? |
| title_full_unstemmed | Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL? |
| title_short | Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL? |
| title_sort | which is the optimum antigen concentration for the venereal disease research laboratory test of cerebrospinal fluid for neurosyphilis diagnosis 10 or 17 μl |
| topic | CSF VDRL antigen concentration neurosyphilis diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.877186/full |
| work_keys_str_mv | AT yaoxiao whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT yaoxiao whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT weili whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT weili whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT qiulingli whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT qiulingli whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT qiuyanxu whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT qiuyanxu whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT yangyang whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT tianciyang whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT tianciyang whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT lililiu whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT lililiu whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml AT weiminggu whichistheoptimumantigenconcentrationforthevenerealdiseaseresearchlaboratorytestofcerebrospinalfluidforneurosyphilisdiagnosis10or17ml |