Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus
BackgroundThe microbiome has been increasingly associated with different disease processes, but its role in esophagus is largely unknown. Our goal was to determine the associations of the esophageal microbiota with Barrett’s esophagus.MethodsA total of 74 patients were included in this prospective s...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.555072/full |
| _version_ | 1818668091119239168 |
|---|---|
| author | Ikenna C. Okereke Aaron L. Miller Daniel C. Jupiter Catherine F. Hamilton Gabriel L. Reep Timothy Krill Clark R. Andersen Richard B. Pyles |
| author_facet | Ikenna C. Okereke Aaron L. Miller Daniel C. Jupiter Catherine F. Hamilton Gabriel L. Reep Timothy Krill Clark R. Andersen Richard B. Pyles |
| author_sort | Ikenna C. Okereke |
| collection | DOAJ |
| description | BackgroundThe microbiome has been increasingly associated with different disease processes, but its role in esophagus is largely unknown. Our goal was to determine the associations of the esophageal microbiota with Barrett’s esophagus.MethodsA total of 74 patients were included in this prospective study, including 34 patients with Barrett’s esophagus and 40 patients without Barrett’s esophagus. Esophageal swabs were obtained from the uvula, and mucosal biopsies were obtained from the proximal esophagus and distal esophagus in each patient. The microbiome of each sample was assessed using a customized Esophageal Microbiome qPCR array (EMB). For each clinical sample, we completed a detection/non-detection analysis for each organism in the EMB. The limit of detection (LOD) for each target was established by analysis of plasmid dilutions.ResultsAverage age was 60.2 years. There were significantly different microbial detection patterns in patients with Barrett’s esophagus compared to the control population. There were a greater number of organisms which had different likelihoods of detection in the distal esophagus, compared to the proximal esophagus or uvula. In addition, as the length of the Barrett’s column increased, multiple organisms were less likely to be detected. This decreased likelihood occurred only in the distal esophagus. Beside Barrett’s esophagus, no other demographic factors were associated with differences in detection patterns.ConclusionsMicrobial community structures differ between patients with and without Barrett’s esophagus. Certain organisms are less likely to be detected as the severity of Barrett’s esophagus worsens. These results suggest that particular organisms may have a protective effect against the development of Barrett’s esophagus. |
| first_indexed | 2024-12-17T06:30:48Z |
| format | Article |
| id | doaj.art-e1707aa161d3493a905e5d5839d7038c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-17T06:30:48Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e1707aa161d3493a905e5d5839d7038c2022-12-21T22:00:09ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-02-011110.3389/fcimb.2021.555072555072Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s EsophagusIkenna C. Okereke0Aaron L. Miller1Daniel C. Jupiter2Catherine F. Hamilton3Gabriel L. Reep4Timothy Krill5Clark R. Andersen6Richard B. Pyles7Division of Cardiothoracic Surgery, University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDepartment of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX , United StatesDepartment of Preventive Medicine and Population Health, University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDivision of Cardiothoracic Surgery, University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDivision of Gastroenterology, University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDivision of Gastroenterology, University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDepartment of Preventive Medicine and Population Health, University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDepartment of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX , United StatesBackgroundThe microbiome has been increasingly associated with different disease processes, but its role in esophagus is largely unknown. Our goal was to determine the associations of the esophageal microbiota with Barrett’s esophagus.MethodsA total of 74 patients were included in this prospective study, including 34 patients with Barrett’s esophagus and 40 patients without Barrett’s esophagus. Esophageal swabs were obtained from the uvula, and mucosal biopsies were obtained from the proximal esophagus and distal esophagus in each patient. The microbiome of each sample was assessed using a customized Esophageal Microbiome qPCR array (EMB). For each clinical sample, we completed a detection/non-detection analysis for each organism in the EMB. The limit of detection (LOD) for each target was established by analysis of plasmid dilutions.ResultsAverage age was 60.2 years. There were significantly different microbial detection patterns in patients with Barrett’s esophagus compared to the control population. There were a greater number of organisms which had different likelihoods of detection in the distal esophagus, compared to the proximal esophagus or uvula. In addition, as the length of the Barrett’s column increased, multiple organisms were less likely to be detected. This decreased likelihood occurred only in the distal esophagus. Beside Barrett’s esophagus, no other demographic factors were associated with differences in detection patterns.ConclusionsMicrobial community structures differ between patients with and without Barrett’s esophagus. Certain organisms are less likely to be detected as the severity of Barrett’s esophagus worsens. These results suggest that particular organisms may have a protective effect against the development of Barrett’s esophagus.https://www.frontiersin.org/articles/10.3389/fcimb.2021.555072/fullBarrett’s esophagusmicrobiomeesophageal cancergastroesophageal reflux diseasemicrobial community |
| spellingShingle | Ikenna C. Okereke Aaron L. Miller Daniel C. Jupiter Catherine F. Hamilton Gabriel L. Reep Timothy Krill Clark R. Andersen Richard B. Pyles Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus Frontiers in Cellular and Infection Microbiology Barrett’s esophagus microbiome esophageal cancer gastroesophageal reflux disease microbial community |
| title | Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus |
| title_full | Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus |
| title_fullStr | Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus |
| title_full_unstemmed | Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus |
| title_short | Microbiota Detection Patterns Correlate With Presence and Severity of Barrett’s Esophagus |
| title_sort | microbiota detection patterns correlate with presence and severity of barrett s esophagus |
| topic | Barrett’s esophagus microbiome esophageal cancer gastroesophageal reflux disease microbial community |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.555072/full |
| work_keys_str_mv | AT ikennacokereke microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT aaronlmiller microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT danielcjupiter microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT catherinefhamilton microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT gabriellreep microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT timothykrill microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT clarkrandersen microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus AT richardbpyles microbiotadetectionpatternscorrelatewithpresenceandseverityofbarrettsesophagus |