Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2
Tailocins are nanomolecular machines with bactericidal activity. They are produced by bacteria to contribute to fitness in mixed communities, and hence, they play a critical role in their ecology in a variety of habitats. Here, we characterized the new tailocin produced by Dickeya dadantii strain 39...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1307349/full |
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| author | Marcin Borowicz Dorota M. Krzyżanowska Magdalena Narajczyk Marta Sobolewska Magdalena Rajewska Paulina Czaplewska Katarzyna Węgrzyn Robert Czajkowski |
| author_facet | Marcin Borowicz Dorota M. Krzyżanowska Magdalena Narajczyk Marta Sobolewska Magdalena Rajewska Paulina Czaplewska Katarzyna Węgrzyn Robert Czajkowski |
| author_sort | Marcin Borowicz |
| collection | DOAJ |
| description | Tailocins are nanomolecular machines with bactericidal activity. They are produced by bacteria to contribute to fitness in mixed communities, and hence, they play a critical role in their ecology in a variety of habitats. Here, we characterized the new tailocin produced by Dickeya dadantii strain 3937, a well-characterized member of plant pathogenic Soft Rot Pectobacteriaceae (SRP). Tailocins induced in D. dadantii were ca. 166 nm long tubes surrounded by contractive sheaths with baseplates having tail fibers at one end. A 22-kb genomic cluster involved in their synthesis and having high homology to the cluster coding for the tail of the Peduovirus P2 was identified. The D. dadantii tailocins, termed dickeyocins P2D1 (phage P2-like dickeyocin 1), were resistant to inactivation by pH (3.5–12), temperature (4–50°C), and elevated osmolarity (NaCl concentration: 0.01–1 M). P2D1 could kill a variety of different Dickeya spp. but not any strain of Pectobacterium spp. tested and were not toxic to Caenorhabditis elegans. |
| first_indexed | 2024-03-09T14:02:24Z |
| format | Article |
| id | doaj.art-e1740918cab3420aa8e3640e1aab49f7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-09T14:02:24Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e1740918cab3420aa8e3640e1aab49f72023-11-30T07:01:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.13073491307349Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2Marcin Borowicz0Dorota M. Krzyżanowska1Magdalena Narajczyk2Marta Sobolewska3Magdalena Rajewska4Paulina Czaplewska5Katarzyna Węgrzyn6Robert Czajkowski7Laboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandLaboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandBioimaging Laboratory, Faculty of Biology, University of Gdańsk, Gdańsk, PolandLaboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandLaboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandLaboratory of Mass Spectrometry-Core Facility Laboratories, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandLaboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandLaboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of UG and MUG, University of Gdańsk, Gdańsk, PolandTailocins are nanomolecular machines with bactericidal activity. They are produced by bacteria to contribute to fitness in mixed communities, and hence, they play a critical role in their ecology in a variety of habitats. Here, we characterized the new tailocin produced by Dickeya dadantii strain 3937, a well-characterized member of plant pathogenic Soft Rot Pectobacteriaceae (SRP). Tailocins induced in D. dadantii were ca. 166 nm long tubes surrounded by contractive sheaths with baseplates having tail fibers at one end. A 22-kb genomic cluster involved in their synthesis and having high homology to the cluster coding for the tail of the Peduovirus P2 was identified. The D. dadantii tailocins, termed dickeyocins P2D1 (phage P2-like dickeyocin 1), were resistant to inactivation by pH (3.5–12), temperature (4–50°C), and elevated osmolarity (NaCl concentration: 0.01–1 M). P2D1 could kill a variety of different Dickeya spp. but not any strain of Pectobacterium spp. tested and were not toxic to Caenorhabditis elegans.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1307349/fullbacteriophagephage tailtailocinErwinia chrysanthemibacteriocinbacteria-bacteria interactions |
| spellingShingle | Marcin Borowicz Dorota M. Krzyżanowska Magdalena Narajczyk Marta Sobolewska Magdalena Rajewska Paulina Czaplewska Katarzyna Węgrzyn Robert Czajkowski Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2 Frontiers in Microbiology bacteriophage phage tail tailocin Erwinia chrysanthemi bacteriocin bacteria-bacteria interactions |
| title | Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2 |
| title_full | Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2 |
| title_fullStr | Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2 |
| title_full_unstemmed | Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2 |
| title_short | Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Peduovirus P2 |
| title_sort | soft rot pathogen dickeya dadantii 3937 produces tailocins resembling the tails of peduovirus p2 |
| topic | bacteriophage phage tail tailocin Erwinia chrysanthemi bacteriocin bacteria-bacteria interactions |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1307349/full |
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