Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection
Objective: To compare the production of pro- and anti-inflammatory cytokines by peripheral blood mononuclear cells (PBMC) from obese but otherwise healthy individuals to that of normal-weight volunteers. Methods: 25 healthy normal-weight subjects and 41 obese individuals were enrolled. Weight and he...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2013-04-01
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| Series: | Obesity Facts |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/350775 |
| _version_ | 1818267061655175168 |
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| author | Dror Dicker Mahmud Abo Salook Dana Marcoviciu Meir Djaldetti Hanna Bessler |
| author_facet | Dror Dicker Mahmud Abo Salook Dana Marcoviciu Meir Djaldetti Hanna Bessler |
| author_sort | Dror Dicker |
| collection | DOAJ |
| description | Objective: To compare the production of pro- and anti-inflammatory cytokines by peripheral blood mononuclear cells (PBMC) from obese but otherwise healthy individuals to that of normal-weight volunteers. Methods: 25 healthy normal-weight subjects and 41 obese individuals were enrolled. Weight and height were measured twice. PBMC were examined for their capacity to generate pro-inflammatory (TNF-α, IFN-γ, IL-1β, IL-6, IL-2) and anti-inflammatory IL-10 and IL-1ra) cytokines. Results: PBMC from obese individuals, compared to those from subjects with normal weight showed an increased production of the pro-inflammatory cytokines IL-2 (6.7 ± 0.4. vs. 4.9 ± 0.3 ng/ml; p = 0.003), TNF-α (505 ± 45 vs. 277 ± 32 pg/ml; p = 0.001), and IFN-γ (93.8 ± 6.0 vs. 73.9 ± 2.7 ng/ml; p = 0.0016). However, PBMC from obese individuals produced a lower amount of the anti-inflammatory cytokine IL-10 (651 ±72 pg/ml) versus those from subjects with normal weight (951 ± 133 pg/ml; p = 0.039). Conclusions: The findings imply that obese individuals are in a ‘low-grade inflammatory state', presumed to be connected with metabolic and cardiovascular co morbidities. The surplus of pro-inflammatory cytokines produced by circulating mononuclear cells of obese individuals, together with those secreted by adipocytes and non-fat cells in the adipose tissue, may contribute to the predisposition of obese patients to inflammation and infections. |
| first_indexed | 2024-12-12T20:16:37Z |
| format | Article |
| id | doaj.art-e174e0d0798a44929864172aa1f19bfe |
| institution | Directory Open Access Journal |
| issn | 1662-4025 1662-4033 |
| language | English |
| last_indexed | 2024-12-12T20:16:37Z |
| publishDate | 2013-04-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Obesity Facts |
| spelling | doaj.art-e174e0d0798a44929864172aa1f19bfe2022-12-22T00:13:22ZengKarger PublishersObesity Facts1662-40251662-40332013-04-016214615110.1159/000350775350775Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and InfectionDror DickerMahmud Abo SalookDana MarcoviciuMeir DjaldettiHanna BesslerObjective: To compare the production of pro- and anti-inflammatory cytokines by peripheral blood mononuclear cells (PBMC) from obese but otherwise healthy individuals to that of normal-weight volunteers. Methods: 25 healthy normal-weight subjects and 41 obese individuals were enrolled. Weight and height were measured twice. PBMC were examined for their capacity to generate pro-inflammatory (TNF-α, IFN-γ, IL-1β, IL-6, IL-2) and anti-inflammatory IL-10 and IL-1ra) cytokines. Results: PBMC from obese individuals, compared to those from subjects with normal weight showed an increased production of the pro-inflammatory cytokines IL-2 (6.7 ± 0.4. vs. 4.9 ± 0.3 ng/ml; p = 0.003), TNF-α (505 ± 45 vs. 277 ± 32 pg/ml; p = 0.001), and IFN-γ (93.8 ± 6.0 vs. 73.9 ± 2.7 ng/ml; p = 0.0016). However, PBMC from obese individuals produced a lower amount of the anti-inflammatory cytokine IL-10 (651 ±72 pg/ml) versus those from subjects with normal weight (951 ± 133 pg/ml; p = 0.039). Conclusions: The findings imply that obese individuals are in a ‘low-grade inflammatory state', presumed to be connected with metabolic and cardiovascular co morbidities. The surplus of pro-inflammatory cytokines produced by circulating mononuclear cells of obese individuals, together with those secreted by adipocytes and non-fat cells in the adipose tissue, may contribute to the predisposition of obese patients to inflammation and infections.http://www.karger.com/Article/FullText/350775CytokinesInflammationObesityPeripheral blood mononuclear cellsInfection |
| spellingShingle | Dror Dicker Mahmud Abo Salook Dana Marcoviciu Meir Djaldetti Hanna Bessler Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection Obesity Facts Cytokines Inflammation Obesity Peripheral blood mononuclear cells Infection |
| title | Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection |
| title_full | Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection |
| title_fullStr | Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection |
| title_full_unstemmed | Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection |
| title_short | Role of Peripheral Blood Mononuclear Cells in the Predisposition of Obese Individuals to Inflammation and Infection |
| title_sort | role of peripheral blood mononuclear cells in the predisposition of obese individuals to inflammation and infection |
| topic | Cytokines Inflammation Obesity Peripheral blood mononuclear cells Infection |
| url | http://www.karger.com/Article/FullText/350775 |
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