Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu
Objective: Development of antimicrobial materials using nano approach and several industries like aquaculture greatly depend on novel biogenic materials. Biogenic techniques to develop nanomaterials with potent antimicrobial activity have been explored recently. The present study demonstrates the gr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721004766 |
| _version_ | 1818157584414146560 |
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| author | Ravi Mani Parameswaran Vijayakumar T. Stalin Dhas Karthick Velu D. Inbakandan C. Thamaraiselvi Babett Greff Murugesan Chandrasekaran Saeedah Musaed Almutairi Faris S Alharbi Dina S. Hussein Maisari Utami |
| author_facet | Ravi Mani Parameswaran Vijayakumar T. Stalin Dhas Karthick Velu D. Inbakandan C. Thamaraiselvi Babett Greff Murugesan Chandrasekaran Saeedah Musaed Almutairi Faris S Alharbi Dina S. Hussein Maisari Utami |
| author_sort | Ravi Mani |
| collection | DOAJ |
| description | Objective: Development of antimicrobial materials using nano approach and several industries like aquaculture greatly depend on novel biogenic materials. Biogenic techniques to develop nanomaterials with potent antimicrobial activity have been explored recently. The present study demonstrates the green synthesis of AgNPs using butter fruit (Persea americana) pulp extract and its antibacterial efficacy against the fish pathogen Providencia vermicola using Rohu fish. Results: The AgNPs were prepared and characterized using various spectroscopic and microscopic techniques. The infrared spectroscopic analysis identified that the fruit biological molecules were involved in the stabilization of AgNPs. Transmission electron microscopic analysis revealed that particles size ranged from 20 to 50 nm. Further, the nanoparticles (5 µg) encapsulated fish feed were given to P. vermicola infected Rohu fish. The survival rate observed was 72 % in experimental group as compared to the control group. Total plate count and histopathological results indicated that the AgNPs treated groups showed significant reduction of bacterial population and restore the tissues in the normal range. Conclusion: The results suggest that the green synthesized (AgNPs) using butter fruit pulp have good efficiency in reducing the infection caused by P. vermicola in Rohu fish. |
| first_indexed | 2024-12-11T15:16:31Z |
| format | Article |
| id | doaj.art-e176aab68ec4457a99ee1b9e71d049ac |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-11T15:16:31Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-e176aab68ec4457a99ee1b9e71d049ac2022-12-22T01:00:34ZengElsevierJournal of King Saud University: Science1018-36472022-04-01343101814Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in RohuRavi Mani0Parameswaran Vijayakumar1T. Stalin Dhas2Karthick Velu3D. Inbakandan4C. Thamaraiselvi5Babett Greff6Murugesan Chandrasekaran7Saeedah Musaed Almutairi8Faris S Alharbi9Dina S. Hussein10Maisari Utami11Centre for Ocean Research (DST-FIST Sponsored Centre) (MoES-ESTC Cell), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, India; Corresponding authors.Centre for Ocean Research (DST-FIST Sponsored Centre) (MoES-ESTC Cell), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, IndiaCentre for Ocean Research (DST-FIST Sponsored Centre) (MoES-ESTC Cell), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, IndiaCentre for Ocean Research (DST-FIST Sponsored Centre) (MoES-ESTC Cell), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, IndiaCentre for Ocean Research (DST-FIST Sponsored Centre) (MoES-ESTC Cell), Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, IndiaDepartment of Biotechnology, Mother Teresa Women’s University, Kodaikanal, Tamil Nadu, India; Corresponding authors.Department of Food Science, Faculty of Agricultural and Food Sciences, Széchenyi István University, 15-17 Lucsony Street, Mosonmagyaróvár 9200, HungaryDepartment of Food Science and Biotechnology, Sejong University, Gwangjin-gu, Seoul 05006, Republic of KoreaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, College of Sciences and Health, Cleveland State University, Cleveland 44115, USADepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia, Yogyakarta, Indonesia; Corresponding authors.Objective: Development of antimicrobial materials using nano approach and several industries like aquaculture greatly depend on novel biogenic materials. Biogenic techniques to develop nanomaterials with potent antimicrobial activity have been explored recently. The present study demonstrates the green synthesis of AgNPs using butter fruit (Persea americana) pulp extract and its antibacterial efficacy against the fish pathogen Providencia vermicola using Rohu fish. Results: The AgNPs were prepared and characterized using various spectroscopic and microscopic techniques. The infrared spectroscopic analysis identified that the fruit biological molecules were involved in the stabilization of AgNPs. Transmission electron microscopic analysis revealed that particles size ranged from 20 to 50 nm. Further, the nanoparticles (5 µg) encapsulated fish feed were given to P. vermicola infected Rohu fish. The survival rate observed was 72 % in experimental group as compared to the control group. Total plate count and histopathological results indicated that the AgNPs treated groups showed significant reduction of bacterial population and restore the tissues in the normal range. Conclusion: The results suggest that the green synthesized (AgNPs) using butter fruit pulp have good efficiency in reducing the infection caused by P. vermicola in Rohu fish.http://www.sciencedirect.com/science/article/pii/S1018364721004766Silver nanoparticlesPersea americanaLabeo rohitaSpectroscopyHistopathology: Antibacterial activity |
| spellingShingle | Ravi Mani Parameswaran Vijayakumar T. Stalin Dhas Karthick Velu D. Inbakandan C. Thamaraiselvi Babett Greff Murugesan Chandrasekaran Saeedah Musaed Almutairi Faris S Alharbi Dina S. Hussein Maisari Utami Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu Journal of King Saud University: Science Silver nanoparticles Persea americana Labeo rohita Spectroscopy Histopathology: Antibacterial activity |
| title | Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu |
| title_full | Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu |
| title_fullStr | Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu |
| title_full_unstemmed | Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu |
| title_short | Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu |
| title_sort | synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against providencia vermicola in rohu |
| topic | Silver nanoparticles Persea americana Labeo rohita Spectroscopy Histopathology: Antibacterial activity |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721004766 |
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