The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro
Background: Spermatogonial stem cells (SSCs) are undifferentiated cells with special capabilities on the self-renewing and differentiation. Proliferation of SSCs is a primary requirement for the study of their characteristics and function in vitro. The aim of this study was to evaluate the effects o...
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Isfahan University of Medical Sciences
2014-03-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2912 |
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| author | Parviz Tajik Mohammad-Reza Mokhber-Dezfuli Nafise Alighazi Peyman Rahimi-Feyli Shiva Shafiei |
| author_facet | Parviz Tajik Mohammad-Reza Mokhber-Dezfuli Nafise Alighazi Peyman Rahimi-Feyli Shiva Shafiei |
| author_sort | Parviz Tajik |
| collection | DOAJ |
| description | Background: Spermatogonial stem cells (SSCs) are undifferentiated cells with special capabilities on the self-renewing and differentiation. Proliferation of SSCs is a primary requirement for the study of their characteristics and function in vitro. The aim of this study was to evaluate the effects of different concentrations of follicle stimulating hormone (FSH) on the colonization activity of ovine SSCs in a short-term in-vitro co-culture with sertoli cells.
Methods: Both sertoli and spermatogonial cells were isolated from 2-3 months old lamb testes by two-steps enzymatic digestion. Afterward, isolated cells were cultured in four groups with different concentrations of FSH (0, 5, 10 and 15 IU/ml, respectively) for 10 days. Colony assay (number and surface area) was evaluated 4, 7 and 10 days after the beginning of the culture by light microscope.
Findings: At the day 4, colony surfaces of groups 1 and 2 were significantly more than groups 3 and 4 (P < 0.05). At the days 7 and 10, colony surfaces of groups 1 and 2 were significantly more than group 4 (P < 0.05).
Conclusion: The results showed that treated groups with 10 and 15 IU/ml of FSH significantly decreased colony surface of ovine SSCs in comparison with co-culture system. |
| first_indexed | 2024-03-12T09:16:26Z |
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| id | doaj.art-e17814d2d8f54da9b73f0c89af17472f |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:16:26Z |
| publishDate | 2014-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-e17814d2d8f54da9b73f0c89af17472f2023-09-02T14:48:50ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2014-03-0131268222822371500The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in VitroParviz Tajik0Mohammad-Reza Mokhber-Dezfuli1Nafise Alighazi2Peyman Rahimi-Feyli3Shiva Shafiei4Professor, Department of Clinical Sciences, School of Veterinary Medicine, University of Tehran, Tehran, IranProfessor, Department of Clinical Sciences, School of Veterinary Medicine, University of Tehran, Tehran, IranVeterinarian, School of Veterinary Medicine, University of Tehran, Tehran, IranAssistant Professor, Department of Clinical Sciences, School of Veterinary Medicine, Razi University, Kermanshah, IranSchool of Veterinary Medicine, University of Tehran, Tehran, IranBackground: Spermatogonial stem cells (SSCs) are undifferentiated cells with special capabilities on the self-renewing and differentiation. Proliferation of SSCs is a primary requirement for the study of their characteristics and function in vitro. The aim of this study was to evaluate the effects of different concentrations of follicle stimulating hormone (FSH) on the colonization activity of ovine SSCs in a short-term in-vitro co-culture with sertoli cells. Methods: Both sertoli and spermatogonial cells were isolated from 2-3 months old lamb testes by two-steps enzymatic digestion. Afterward, isolated cells were cultured in four groups with different concentrations of FSH (0, 5, 10 and 15 IU/ml, respectively) for 10 days. Colony assay (number and surface area) was evaluated 4, 7 and 10 days after the beginning of the culture by light microscope. Findings: At the day 4, colony surfaces of groups 1 and 2 were significantly more than groups 3 and 4 (P < 0.05). At the days 7 and 10, colony surfaces of groups 1 and 2 were significantly more than group 4 (P < 0.05). Conclusion: The results showed that treated groups with 10 and 15 IU/ml of FSH significantly decreased colony surface of ovine SSCs in comparison with co-culture system.http://jims.mui.ac.ir/index.php/jims/article/view/2912SpermatogoniaStem cellSertoli cellFollicle stimulating hormone (FSH)Ovine |
| spellingShingle | Parviz Tajik Mohammad-Reza Mokhber-Dezfuli Nafise Alighazi Peyman Rahimi-Feyli Shiva Shafiei The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro مجله دانشکده پزشکی اصفهان Spermatogonia Stem cell Sertoli cell Follicle stimulating hormone (FSH) Ovine |
| title | The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro |
| title_full | The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro |
| title_fullStr | The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro |
| title_full_unstemmed | The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro |
| title_short | The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro |
| title_sort | effect of follicle stimulating hormone fsh on colony formation of ovine spermatogonial stem cells in vitro |
| topic | Spermatogonia Stem cell Sertoli cell Follicle stimulating hormone (FSH) Ovine |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2912 |
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