IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”
Stem node explants of three almond (Prunus dulcis Mill) cultivars (Om – Elfahm, M-Dalt and Ne Plus Ultra) and the hybrid rootstock “Hansen” cv. were suc-cessfully established and proliferated on Murashige and Skoog medium (MS) sup-plemented with benzyl adenine (BA) at 0.0 - 2.0 mgl -1 and indol buty...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2005-09-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_15512_f80187bc6c24750d4ab930b050b781b2.pdf |
| _version_ | 1797269701379751936 |
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| author | A El-Hammady Wafaa Wanas A Abd El-Hamid M El-Salem |
| author_facet | A El-Hammady Wafaa Wanas A Abd El-Hamid M El-Salem |
| author_sort | A El-Hammady |
| collection | DOAJ |
| description | Stem node explants of three almond (Prunus dulcis Mill) cultivars (Om – Elfahm, M-Dalt and Ne Plus Ultra) and the hybrid rootstock “Hansen” cv. were suc-cessfully established and proliferated on Murashige and Skoog medium (MS) sup-plemented with benzyl adenine (BA) at 0.0 - 2.0 mgl -1 and indol butyric acid (IBA) at 0.0 - 0.1 mgl-1 .The highest bud development percentage of stem node explant was obtained with medium contained BA at 2 mgl-1 with or without IBA at 0.1 mgl-1 for M – Dalet and Ne Plus Ultra almond cultivars compared with other treatments. The longest shoot during establishment stage was recorded by “Hansen” rootstock cultured in MS with BA at 2.0 mgl-1 plus IBA at 0.01 mgl-1.Using BA at 1.0 mgl-1 plus IBA at 0.1 mgl-1 and BA at 2.0 mgl -1 plus 0.05 or 0.1 mgl -1gave the highest number of proliferated shoots for Om – Elfahm, M-Dalt and Ne Puls Ultra cvs. and “Hansen”, respectively in the 3rd subculture. The best average proliferated shoot length was achieved with medium supplemented with BA at 1.0 mgl-1 plus IBA at 0.05 mgl-1 or 0.1 mgl-1 for M-Dalt, Ne Puls Ultra and “Hansen” respectively, where-as, using BA at 2.0 mgl -1 plus IBA at 0.1 mgl -1 recorded the longest average prolif-erated shoot for Om- Elfahm cultivar. Microshoots were rooted in modified Bourgin and Nitsch medium supplemented with IBA or NAA at 1.0 mgl-1 only or combination (0.2 IBA mgl-1 + NAA 0.4 mgl-1, IBA 0.4 mgl-1 + NAA 0.8 mgl-1 and IBA 0.5 mgl-1 + NAA 1.0 mgl-1). The large callus (more than 10 mm diameter) was recorded with “Hansen” rootstock and Om – Elfahm treated by IBA at 0.4 mgl -1 plus NAA at 0.8 mgl -1. The greatest rooting percentage (66.67%) was obtained with medium contained IBA at 0.2 or 0.4 mgl-1 plus NAA at 0.4 or 0.8 mgl-1 for “Hansen” rootstock and Ne Plus Ultra shoots. |
| first_indexed | 2024-04-25T01:52:33Z |
| format | Article |
| id | doaj.art-e1797dee4b5c46a0b30e6ab744606f0a |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:52:33Z |
| publishDate | 2005-09-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-e1797dee4b5c46a0b30e6ab744606f0a2024-03-07T17:48:13ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852005-09-0113248149910.21608/ajs.2005.1551215512IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN”A El-Hammady0Wafaa Wanas1A Abd El-Hamid2M El-Salem3Department of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, EgyptDepartment of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, EgyptDepartment of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, Egypt.Department of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, EgyptStem node explants of three almond (Prunus dulcis Mill) cultivars (Om – Elfahm, M-Dalt and Ne Plus Ultra) and the hybrid rootstock “Hansen” cv. were suc-cessfully established and proliferated on Murashige and Skoog medium (MS) sup-plemented with benzyl adenine (BA) at 0.0 - 2.0 mgl -1 and indol butyric acid (IBA) at 0.0 - 0.1 mgl-1 .The highest bud development percentage of stem node explant was obtained with medium contained BA at 2 mgl-1 with or without IBA at 0.1 mgl-1 for M – Dalet and Ne Plus Ultra almond cultivars compared with other treatments. The longest shoot during establishment stage was recorded by “Hansen” rootstock cultured in MS with BA at 2.0 mgl-1 plus IBA at 0.01 mgl-1.Using BA at 1.0 mgl-1 plus IBA at 0.1 mgl-1 and BA at 2.0 mgl -1 plus 0.05 or 0.1 mgl -1gave the highest number of proliferated shoots for Om – Elfahm, M-Dalt and Ne Puls Ultra cvs. and “Hansen”, respectively in the 3rd subculture. The best average proliferated shoot length was achieved with medium supplemented with BA at 1.0 mgl-1 plus IBA at 0.05 mgl-1 or 0.1 mgl-1 for M-Dalt, Ne Puls Ultra and “Hansen” respectively, where-as, using BA at 2.0 mgl -1 plus IBA at 0.1 mgl -1 recorded the longest average prolif-erated shoot for Om- Elfahm cultivar. Microshoots were rooted in modified Bourgin and Nitsch medium supplemented with IBA or NAA at 1.0 mgl-1 only or combination (0.2 IBA mgl-1 + NAA 0.4 mgl-1, IBA 0.4 mgl-1 + NAA 0.8 mgl-1 and IBA 0.5 mgl-1 + NAA 1.0 mgl-1). The large callus (more than 10 mm diameter) was recorded with “Hansen” rootstock and Om – Elfahm treated by IBA at 0.4 mgl -1 plus NAA at 0.8 mgl -1. The greatest rooting percentage (66.67%) was obtained with medium contained IBA at 0.2 or 0.4 mgl-1 plus NAA at 0.4 or 0.8 mgl-1 for “Hansen” rootstock and Ne Plus Ultra shoots.https://ajs.journals.ekb.eg/article_15512_f80187bc6c24750d4ab930b050b781b2.pdfpropagationalmond cvsbaibamultiplicationsubculturerooting |
| spellingShingle | A El-Hammady Wafaa Wanas A Abd El-Hamid M El-Salem IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN” Arab Universities Journal of Agricultural Sciences propagation almond cvs ba iba multiplication subculture rooting |
| title | IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN” |
| title_full | IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN” |
| title_fullStr | IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN” |
| title_full_unstemmed | IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN” |
| title_short | IN VITRO PROPAGATION OF THREE ALMOND CULTIVARS AND THE ALMOND – PEACH HYBRID ROOT-STOCK“HANSEN” |
| title_sort | in vitro propagation of three almond cultivars and the almond peach hybrid root stock hansen |
| topic | propagation almond cvs ba iba multiplication subculture rooting |
| url | https://ajs.journals.ekb.eg/article_15512_f80187bc6c24750d4ab930b050b781b2.pdf |
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