The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation
Abstract Background Evidence supports an association between cholestatic liver disease and changes in microbiome composition. Nevertheless, the identification of this special type of biliary atresia from non-biliary atresia cholestasis is still a major clinical difficulty. The purpose of this study...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Pediatrics |
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| Online Access: | https://doi.org/10.1186/s12887-022-03570-1 |
| _version_ | 1828356550904774656 |
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| author | Xinhe Sun Yaoyao Cai Wenwen Dai Weiwei Jiang Weibing Tang |
| author_facet | Xinhe Sun Yaoyao Cai Wenwen Dai Weiwei Jiang Weibing Tang |
| author_sort | Xinhe Sun |
| collection | DOAJ |
| description | Abstract Background Evidence supports an association between cholestatic liver disease and changes in microbiome composition. Nevertheless, the identification of this special type of biliary atresia from non-biliary atresia cholestasis is still a major clinical difficulty. The purpose of this study is to compare the differences in the composition of gut microbiome between infants with biliary atresia and infant with non-biliary atrestic cholestasis, to find new ways to identify and diagnose these two diseases early, to understand the influence of the presence or absence of bile on the composition of the gut microbiome in infants with cholestasis. Methods Using 16S rDNA gene sequencing technology to analyze the intestinal flora of the participants. Results In terms of diversity, there is an obvious structural separation in the intestinal microbiota of the BA group and the CD group, and this structural separation also exists in the comparison between the two groups before surgery. Taxonomic analysis demonstrated that the two groups showed an increase in Proteobacteria and Firmicutes before surgery, and the relative abundance of potential pathogens such as Shigella, Streptococcus, Klebsiella, etc. increased, potential probiotics such as Bifidobacteria and Lactobacillus decreased, but the relative abundance of each genus was different between groups. It was found that Enterococcus, Ralstonia, Nitriliruptoraceae, etc. were differentially enriched in the BA group, the CD group are mainly enriched in Veillonella, Clostridium_sensu_stricto_1 and Lactobacillus. Functional analysis of the groups showed that the BA group mainly focused on the processes of energy release processes, and the CD group mainly focused on the biosynthesis of amino-acids to consume energy. Conclusions The composition of intestinal flora is different between biliary atresia and non-biliary atretic cholestasis. Enterococcus, Ralstonia, etc. may become biomarkers for the identification and diagnosis of both. |
| first_indexed | 2024-04-14T03:00:51Z |
| format | Article |
| id | doaj.art-e1799ecc9f7c4dfaa722ce2453f63031 |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-04-14T03:00:51Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-e1799ecc9f7c4dfaa722ce2453f630312022-12-22T02:15:55ZengBMCBMC Pediatrics1471-24312022-08-0122111110.1186/s12887-022-03570-1The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operationXinhe Sun0Yaoyao Cai1Wenwen Dai2Weiwei Jiang3Weibing Tang4Department of Pediatric Surgery, Children’s Hospital of Nanjing Medical UniversityDepartment of Pediatric Surgery, Children’s Hospital of Nanjing Medical UniversityDepartment of Pediatric Surgery, Yancheng Maternity and Child Health Care HospitalDepartment of Pediatric Surgery, Children’s Hospital of Nanjing Medical UniversityDepartment of Pediatric Surgery, Children’s Hospital of Nanjing Medical UniversityAbstract Background Evidence supports an association between cholestatic liver disease and changes in microbiome composition. Nevertheless, the identification of this special type of biliary atresia from non-biliary atresia cholestasis is still a major clinical difficulty. The purpose of this study is to compare the differences in the composition of gut microbiome between infants with biliary atresia and infant with non-biliary atrestic cholestasis, to find new ways to identify and diagnose these two diseases early, to understand the influence of the presence or absence of bile on the composition of the gut microbiome in infants with cholestasis. Methods Using 16S rDNA gene sequencing technology to analyze the intestinal flora of the participants. Results In terms of diversity, there is an obvious structural separation in the intestinal microbiota of the BA group and the CD group, and this structural separation also exists in the comparison between the two groups before surgery. Taxonomic analysis demonstrated that the two groups showed an increase in Proteobacteria and Firmicutes before surgery, and the relative abundance of potential pathogens such as Shigella, Streptococcus, Klebsiella, etc. increased, potential probiotics such as Bifidobacteria and Lactobacillus decreased, but the relative abundance of each genus was different between groups. It was found that Enterococcus, Ralstonia, Nitriliruptoraceae, etc. were differentially enriched in the BA group, the CD group are mainly enriched in Veillonella, Clostridium_sensu_stricto_1 and Lactobacillus. Functional analysis of the groups showed that the BA group mainly focused on the processes of energy release processes, and the CD group mainly focused on the biosynthesis of amino-acids to consume energy. Conclusions The composition of intestinal flora is different between biliary atresia and non-biliary atretic cholestasis. Enterococcus, Ralstonia, etc. may become biomarkers for the identification and diagnosis of both.https://doi.org/10.1186/s12887-022-03570-1Biliary atresiaCholestaticGut microbiomeIntestinal flora16S rDNA |
| spellingShingle | Xinhe Sun Yaoyao Cai Wenwen Dai Weiwei Jiang Weibing Tang The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation BMC Pediatrics Biliary atresia Cholestatic Gut microbiome Intestinal flora 16S rDNA |
| title | The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation |
| title_full | The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation |
| title_fullStr | The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation |
| title_full_unstemmed | The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation |
| title_short | The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation |
| title_sort | difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation |
| topic | Biliary atresia Cholestatic Gut microbiome Intestinal flora 16S rDNA |
| url | https://doi.org/10.1186/s12887-022-03570-1 |
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