IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells
In mammals, the tachykinin 3 (TAC3)/tachykinin receptor 3 (TACR3) systems have been confirmed to play an important role in the regulation of puberty onset. Using grass carp pituitary cells as the model, our recent study found that the TAC3 gene products could significantly induce somatolactin &#...
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MDPI AG
2019-08-01
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| 丛编: | Cells |
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| 在线阅读: | https://www.mdpi.com/2073-4409/8/8/887 |
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| author | Guangfu Hu Mulan He Wendy K. W. Ko Cheng Ye Qiongyao Hu Anderson O. L. Wong |
| author_facet | Guangfu Hu Mulan He Wendy K. W. Ko Cheng Ye Qiongyao Hu Anderson O. L. Wong |
| author_sort | Guangfu Hu |
| collection | DOAJ |
| description | In mammals, the tachykinin 3 (TAC3)/tachykinin receptor 3 (TACR3) systems have been confirmed to play an important role in the regulation of puberty onset. Using grass carp pituitary cells as the model, our recent study found that the TAC3 gene products could significantly induce somatolactin α (SLα) synthesis and secretion via TACR3 activation. In the present study, we seek to examine if pituitary TACR3 can serve as a regulatory target and contribute to TAC3 interactions with other SLα regulators. Firstly, grass carp TACR3 was cloned and tissue distribution showed that it could be highly detected in grass carp pituitary. Using HEK293 cells as the model, functional expression also revealed that grass carp TACR3 exhibited ligand binding selectivity and post-receptor signaling highly comparable to its mammalian counterpart. Using grass carp pituitary cells as the model, TACR3 mRNA expression could be stimulated by insulin-like growth factor (IGF)-I and -II via the IGF-I receptor coupled to phosphatidylinositol-3-kinase (PI<sub>3</sub>K)/protein kinase B (Akt) and mitogen-activated protein kinase (MAPK) pathways. Interestingly, IGF-I/-II cotreatment could also significantly enhance TAC3-induced SLα mRNA expression and the potentiating effect was dependent on TACR3 expression and activation of adenylate cyclase (AC)/cAMP/protein kinase A (PKA), phospholipase C (PLC)/inositol 1,4,5-triphosphate (IP<sub>3</sub>)/protein kinase C (PKC), and Ca<sup>2+</sup>/calmodulin (CaM)/calmodulin-dependent protein kinase II (CaMK-II) cascades. Besides, IGF-I-induced Akt phosphorylation but not MEK, extracellular signal-regulated kinase (ERK<sub>1/2</sub>), and P<sub>38</sub>MAPK phosphorylation was notably enhanced by TACR3 activation. These results, as a whole, suggest that the potentiating effect of IGFs on TAC3 gene products-induced SLα mRNA expression was mediated by TACR3 upregulation and functional crosstalk of post-receptor signaling in the pituitary. |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T10:40:10Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-e17a2929562c4528a05f38c47d7ae2322023-09-02T08:14:39ZengMDPI AGCells2073-44092019-08-018888710.3390/cells8080887cells8080887IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary CellsGuangfu Hu0Mulan He1Wendy K. W. Ko2Cheng Ye3Qiongyao Hu4Anderson O. L. Wong5College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaSchool of Biological Sciences, University of Hong Kong, Hong Kong, ChinaSchool of Biological Sciences, University of Hong Kong, Hong Kong, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaSchool of Biological Sciences, University of Hong Kong, Hong Kong, ChinaIn mammals, the tachykinin 3 (TAC3)/tachykinin receptor 3 (TACR3) systems have been confirmed to play an important role in the regulation of puberty onset. Using grass carp pituitary cells as the model, our recent study found that the TAC3 gene products could significantly induce somatolactin α (SLα) synthesis and secretion via TACR3 activation. In the present study, we seek to examine if pituitary TACR3 can serve as a regulatory target and contribute to TAC3 interactions with other SLα regulators. Firstly, grass carp TACR3 was cloned and tissue distribution showed that it could be highly detected in grass carp pituitary. Using HEK293 cells as the model, functional expression also revealed that grass carp TACR3 exhibited ligand binding selectivity and post-receptor signaling highly comparable to its mammalian counterpart. Using grass carp pituitary cells as the model, TACR3 mRNA expression could be stimulated by insulin-like growth factor (IGF)-I and -II via the IGF-I receptor coupled to phosphatidylinositol-3-kinase (PI<sub>3</sub>K)/protein kinase B (Akt) and mitogen-activated protein kinase (MAPK) pathways. Interestingly, IGF-I/-II cotreatment could also significantly enhance TAC3-induced SLα mRNA expression and the potentiating effect was dependent on TACR3 expression and activation of adenylate cyclase (AC)/cAMP/protein kinase A (PKA), phospholipase C (PLC)/inositol 1,4,5-triphosphate (IP<sub>3</sub>)/protein kinase C (PKC), and Ca<sup>2+</sup>/calmodulin (CaM)/calmodulin-dependent protein kinase II (CaMK-II) cascades. Besides, IGF-I-induced Akt phosphorylation but not MEK, extracellular signal-regulated kinase (ERK<sub>1/2</sub>), and P<sub>38</sub>MAPK phosphorylation was notably enhanced by TACR3 activation. These results, as a whole, suggest that the potentiating effect of IGFs on TAC3 gene products-induced SLα mRNA expression was mediated by TACR3 upregulation and functional crosstalk of post-receptor signaling in the pituitary.https://www.mdpi.com/2073-4409/8/8/887signal transductiontachykinin receptorpharmacological testneurokinin Bgrass carp |
| spellingShingle | Guangfu Hu Mulan He Wendy K. W. Ko Cheng Ye Qiongyao Hu Anderson O. L. Wong IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells Cells signal transduction tachykinin receptor pharmacological test neurokinin B grass carp |
| title | IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells |
| title_full | IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells |
| title_fullStr | IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells |
| title_full_unstemmed | IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells |
| title_short | IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells |
| title_sort | igfs potentiate tac3 induced slα expression via upregulation of tacr3 expression in grass carp pituitary cells |
| topic | signal transduction tachykinin receptor pharmacological test neurokinin B grass carp |
| url | https://www.mdpi.com/2073-4409/8/8/887 |
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