Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew
The role of reactive oxygen intermediate (ROI) accumulation in resistance and susceptibility of plants to parasitic fungi is still little understood. We examined the spatial and temporal occurrence of different ROIs in barley after inoculation with the biotrophic fungus Blumeria graminis f.sp. horde...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-12-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200211-0063_functional-studies-on-the-role-of-reactive-oxygen-intermediates-in-the-resistance-of-barley-against-powdery-mil.php |
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| author | R. Hückelhoven M. Trujillo C. Dechert H. Schultheiss K.-H. Kogel |
| author_facet | R. Hückelhoven M. Trujillo C. Dechert H. Schultheiss K.-H. Kogel |
| author_sort | R. Hückelhoven |
| collection | DOAJ |
| description | The role of reactive oxygen intermediate (ROI) accumulation in resistance and susceptibility of plants to parasitic fungi is still little understood. We examined the spatial and temporal occurrence of different ROIs in barley after inoculation with the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh, barley powdery mildew fungus). Using histochemical analyses, we collected correlative data indicating that H2O2 and O2*- play different roles in background penetration resistance to Bgh. To study the role of O2*- in detail, we isolated barley cDNAs encoding a NADPH oxidase GP91PHOX homologue and a RACB homologue, which may be involved in NADPH oxidase activation. Interestingly, transient silencing of RACB or GP91PHOX via sequence-specific RNA interference enhanced penetration resistance of barley to Bgh. Together, data reveal rather a negative than a positive role of superoxide generation in background resistance of barley to Bgh. |
| first_indexed | 2024-04-10T08:16:45Z |
| format | Article |
| id | doaj.art-e17d740789fa484c9aebd424e2029d69 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:16:45Z |
| publishDate | 2002-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-e17d740789fa484c9aebd424e2029d692023-02-23T03:43:40ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412002-12-0138SI 2 - 6th Conf EFPP45846010.17221/10523-PPSpps-200211-0063Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildewR. Hückelhoven0M. Trujillo1C. Dechert2H. Schultheiss3K.-H. Kogel4Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyInstitute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyInstitute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyInstitute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyInstitute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, D-35392 Giessen, GermanyThe role of reactive oxygen intermediate (ROI) accumulation in resistance and susceptibility of plants to parasitic fungi is still little understood. We examined the spatial and temporal occurrence of different ROIs in barley after inoculation with the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh, barley powdery mildew fungus). Using histochemical analyses, we collected correlative data indicating that H2O2 and O2*- play different roles in background penetration resistance to Bgh. To study the role of O2*- in detail, we isolated barley cDNAs encoding a NADPH oxidase GP91PHOX homologue and a RACB homologue, which may be involved in NADPH oxidase activation. Interestingly, transient silencing of RACB or GP91PHOX via sequence-specific RNA interference enhanced penetration resistance of barley to Bgh. Together, data reveal rather a negative than a positive role of superoxide generation in background resistance of barley to Bgh.https://pps.agriculturejournals.cz/artkey/pps-200211-0063_functional-studies-on-the-role-of-reactive-oxygen-intermediates-in-the-resistance-of-barley-against-powdery-mil.phpbarleybackground resistanceblumeria graminis f.sp. hordeihordeum vulgarepapillagene silencingrna interference |
| spellingShingle | R. Hückelhoven M. Trujillo C. Dechert H. Schultheiss K.-H. Kogel Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew Plant Protection Science barley background resistance blumeria graminis f.sp. hordei hordeum vulgare papilla gene silencing rna interference |
| title | Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew |
| title_full | Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew |
| title_fullStr | Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew |
| title_full_unstemmed | Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew |
| title_short | Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew |
| title_sort | functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew |
| topic | barley background resistance blumeria graminis f.sp. hordei hordeum vulgare papilla gene silencing rna interference |
| url | https://pps.agriculturejournals.cz/artkey/pps-200211-0063_functional-studies-on-the-role-of-reactive-oxygen-intermediates-in-the-resistance-of-barley-against-powdery-mil.php |
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