Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model
The study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). <i>Mus musculus</i> virgin females were divided into six groups treated with (1) olive oil (10 m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/16/2/174 |
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| author | Michely Laiany Vieira Moura Ag-Anne Pereira Melo de Menezes José Williams Gomes de Oliveira Filho Maria Luiza Lima Barreto do Nascimento Antonielly Campinho dos Reis Alessandra Braga Ribeiro Felipe Cavalcanti Carneiro da Silva Adriana Maria Viana Nunes Hercília Maria Lins Rolim Ana Amélia de Carvalho Melo Cavalcante João Marcelo de Castro e Sousa |
| author_facet | Michely Laiany Vieira Moura Ag-Anne Pereira Melo de Menezes José Williams Gomes de Oliveira Filho Maria Luiza Lima Barreto do Nascimento Antonielly Campinho dos Reis Alessandra Braga Ribeiro Felipe Cavalcanti Carneiro da Silva Adriana Maria Viana Nunes Hercília Maria Lins Rolim Ana Amélia de Carvalho Melo Cavalcante João Marcelo de Castro e Sousa |
| author_sort | Michely Laiany Vieira Moura |
| collection | DOAJ |
| description | The study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). <i>Mus musculus</i> virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 μg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies. |
| first_indexed | 2024-03-07T22:18:22Z |
| format | Article |
| id | doaj.art-e17f14fd78cd451fa10afa1255fc3266 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-07T22:18:22Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-e17f14fd78cd451fa10afa1255fc32662024-02-23T15:30:59ZengMDPI AGPharmaceutics1999-49232024-01-0116217410.3390/pharmaceutics16020174Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer ModelMichely Laiany Vieira Moura0Ag-Anne Pereira Melo de Menezes1José Williams Gomes de Oliveira Filho2Maria Luiza Lima Barreto do Nascimento3Antonielly Campinho dos Reis4Alessandra Braga Ribeiro5Felipe Cavalcanti Carneiro da Silva6Adriana Maria Viana Nunes7Hercília Maria Lins Rolim8Ana Amélia de Carvalho Melo Cavalcante9João Marcelo de Castro e Sousa10Laboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilDepartment of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Pharmaceutical Nanosystems—NANOSFAR, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilLaboratory of Toxicological Genetics—LAPGENIC, Graduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina 64049-550, BrazilThe study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). <i>Mus musculus</i> virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 μg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies.https://www.mdpi.com/1999-4923/16/2/174fungal metabolitescytotoxicitynanotechnologybreast cancer |
| spellingShingle | Michely Laiany Vieira Moura Ag-Anne Pereira Melo de Menezes José Williams Gomes de Oliveira Filho Maria Luiza Lima Barreto do Nascimento Antonielly Campinho dos Reis Alessandra Braga Ribeiro Felipe Cavalcanti Carneiro da Silva Adriana Maria Viana Nunes Hercília Maria Lins Rolim Ana Amélia de Carvalho Melo Cavalcante João Marcelo de Castro e Sousa Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model Pharmaceutics fungal metabolites cytotoxicity nanotechnology breast cancer |
| title | Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model |
| title_full | Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model |
| title_fullStr | Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model |
| title_full_unstemmed | Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model |
| title_short | Advances in Antitumor Effects Using Liposomal Citrinin in Induced Breast Cancer Model |
| title_sort | advances in antitumor effects using liposomal citrinin in induced breast cancer model |
| topic | fungal metabolites cytotoxicity nanotechnology breast cancer |
| url | https://www.mdpi.com/1999-4923/16/2/174 |
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