Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman
<em>Watermelon chlorotic stunt virus</em> (WmCSV) is a bipartite begomovirus (genus <em>Begomovirus</em>, family <em>Geminiviridae</em>) that causes economic losses to cucurbits, particularly watermelon, across the M...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-07-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4915/4/7/1169 |
| _version_ | 1818983045657526272 |
|---|---|
| author | Shahid Mansoor Abdulrahman M. Al-Matrooshi Um Ammara Sohail Akhtar Rob W. Briddon Akhtar J. Khan |
| author_facet | Shahid Mansoor Abdulrahman M. Al-Matrooshi Um Ammara Sohail Akhtar Rob W. Briddon Akhtar J. Khan |
| author_sort | Shahid Mansoor |
| collection | DOAJ |
| description | <em>Watermelon chlorotic stunt virus</em> (WmCSV) is a bipartite begomovirus (genus <em>Begomovirus</em>, family <em>Geminiviridae</em>) that causes economic losses to cucurbits, particularly watermelon, across the Middle East and North Africa. Recently squash (<em>Cucurbita moschata</em>) grown in an experimental field in Oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. Sequence analysis of the virus isolated from squash showed 97.6–99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component and 93–98% identity for the DNA B component. <em>Agrobacterium</em>-mediated inoculation to <em>Nicotiana</em> <em>benthamiana</em> resulted in the development of symptoms fifteen days post inoculation. This is the first bipartite begomovirus identified in Oman. Overall the Oman isolate showed the highest levels of sequence identity to a WmCSV isolate originating from Iran, which was confirmed by phylogenetic analysis. This suggests that WmCSV present in Oman has been introduced from Iran. The significance of this finding is discussed. |
| first_indexed | 2024-12-20T17:56:52Z |
| format | Article |
| id | doaj.art-e17fc668eeaf41e1aeb9167a6574c6d0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-20T17:56:52Z |
| publishDate | 2012-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e17fc668eeaf41e1aeb9167a6574c6d02022-12-21T19:30:44ZengMDPI AGViruses1999-49152012-07-01471169118110.3390/v4071169Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from OmanShahid MansoorAbdulrahman M. Al-MatrooshiUm AmmaraSohail AkhtarRob W. BriddonAkhtar J. Khan<em>Watermelon chlorotic stunt virus</em> (WmCSV) is a bipartite begomovirus (genus <em>Begomovirus</em>, family <em>Geminiviridae</em>) that causes economic losses to cucurbits, particularly watermelon, across the Middle East and North Africa. Recently squash (<em>Cucurbita moschata</em>) grown in an experimental field in Oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. Sequence analysis of the virus isolated from squash showed 97.6–99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component and 93–98% identity for the DNA B component. <em>Agrobacterium</em>-mediated inoculation to <em>Nicotiana</em> <em>benthamiana</em> resulted in the development of symptoms fifteen days post inoculation. This is the first bipartite begomovirus identified in Oman. Overall the Oman isolate showed the highest levels of sequence identity to a WmCSV isolate originating from Iran, which was confirmed by phylogenetic analysis. This suggests that WmCSV present in Oman has been introduced from Iran. The significance of this finding is discussed.http://www.mdpi.com/1999-4915/4/7/1169WmCSV<em>Begomovirus</em>squashOman |
| spellingShingle | Shahid Mansoor Abdulrahman M. Al-Matrooshi Um Ammara Sohail Akhtar Rob W. Briddon Akhtar J. Khan Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman Viruses WmCSV <em>Begomovirus</em> squash Oman |
| title | Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman |
| title_full | Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman |
| title_fullStr | Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman |
| title_full_unstemmed | Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman |
| title_short | Complete Nucleotide Sequence of Watermelon Chlorotic Stunt Virus Originating from Oman |
| title_sort | complete nucleotide sequence of watermelon chlorotic stunt virus originating from oman |
| topic | WmCSV <em>Begomovirus</em> squash Oman |
| url | http://www.mdpi.com/1999-4915/4/7/1169 |
| work_keys_str_mv | AT shahidmansoor completenucleotidesequenceofwatermelonchloroticstuntvirusoriginatingfromoman AT abdulrahmanmalmatrooshi completenucleotidesequenceofwatermelonchloroticstuntvirusoriginatingfromoman AT umammara completenucleotidesequenceofwatermelonchloroticstuntvirusoriginatingfromoman AT sohailakhtar completenucleotidesequenceofwatermelonchloroticstuntvirusoriginatingfromoman AT robwbriddon completenucleotidesequenceofwatermelonchloroticstuntvirusoriginatingfromoman AT akhtarjkhan completenucleotidesequenceofwatermelonchloroticstuntvirusoriginatingfromoman |