pH effect on stability and kinetics degradation of nitazoxanide in solution
Stability studies correspond to a set of tests designed to assess changes in the quality of a given drug over time and under the influence of a number of factors. Among these factors, pH plays an important role, due to the catalytic effect that hydronium and hydroxide ions can play in several react...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal do Rio Grande do Sul
2020-07-01
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| Series: | Drug Analytical Research |
| Online Access: | https://seer.ufrgs.br/index.php/poled/management/settings/website/index.php/dar/article/view/101465 |
| _version_ | 1797318082497085440 |
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| author | Fábio Barbosa Leonardo Pezzi Julia Sorrentino Martin Steppe Nadia Volpato Andreas Sebastian Loureiro Mendez |
| author_facet | Fábio Barbosa Leonardo Pezzi Julia Sorrentino Martin Steppe Nadia Volpato Andreas Sebastian Loureiro Mendez |
| author_sort | Fábio Barbosa |
| collection | DOAJ |
| description |
Stability studies correspond to a set of tests designed to assess changes in the quality of a given drug over time and under the influence of a number of factors. Among these factors, pH plays an important role, due to the catalytic effect that hydronium and hydroxide ions can play in several reactions. In the present study, the degradation kinetics of nitazoxanide was evaluated over a wide pH range, and the main degradation product generated was identified by LC-MS/MS. Nitazoxanide showed first-order degradation kinetics in the pH range of 0.01 to 10.0 showing greater stability between pH 1.0 and 4.0. The degradation rate constant calculated for these pH was 0.0885 x 10-2 min-1 and 0.0689 x 10-2 min-1, respectively. The highest degradation rate constant value was observed at pH 10.0 (0.7418 x 10-2 min-1) followed by pH 0.01 (0.5882 x 10-2 min-1). A major degradation product (DP-1) was observed in all conditions tested. Through LC-MS/MS analysis, DP-1 was identified as a product of nitazoxanide deacetylation. The effect of pH on the stability of nitazoxanide and the kinetic data obtained contribute to a better understanding of the intrinsic stability characteristics of nitazoxanide.
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| first_indexed | 2024-03-08T03:45:21Z |
| format | Article |
| id | doaj.art-e184afdfdf2245529d190123a7ed9593 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-03-08T03:45:21Z |
| publishDate | 2020-07-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-e184afdfdf2245529d190123a7ed95932024-02-09T18:14:42ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162020-07-0141pH effect on stability and kinetics degradation of nitazoxanide in solutionFábio Barbosa0Leonardo Pezzi1Julia Sorrentino2Martin Steppe3Nadia Volpato4Andreas Sebastian Loureiro Mendez5Universidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do Sul Stability studies correspond to a set of tests designed to assess changes in the quality of a given drug over time and under the influence of a number of factors. Among these factors, pH plays an important role, due to the catalytic effect that hydronium and hydroxide ions can play in several reactions. In the present study, the degradation kinetics of nitazoxanide was evaluated over a wide pH range, and the main degradation product generated was identified by LC-MS/MS. Nitazoxanide showed first-order degradation kinetics in the pH range of 0.01 to 10.0 showing greater stability between pH 1.0 and 4.0. The degradation rate constant calculated for these pH was 0.0885 x 10-2 min-1 and 0.0689 x 10-2 min-1, respectively. The highest degradation rate constant value was observed at pH 10.0 (0.7418 x 10-2 min-1) followed by pH 0.01 (0.5882 x 10-2 min-1). A major degradation product (DP-1) was observed in all conditions tested. Through LC-MS/MS analysis, DP-1 was identified as a product of nitazoxanide deacetylation. The effect of pH on the stability of nitazoxanide and the kinetic data obtained contribute to a better understanding of the intrinsic stability characteristics of nitazoxanide. https://seer.ufrgs.br/index.php/poled/management/settings/website/index.php/dar/article/view/101465 |
| spellingShingle | Fábio Barbosa Leonardo Pezzi Julia Sorrentino Martin Steppe Nadia Volpato Andreas Sebastian Loureiro Mendez pH effect on stability and kinetics degradation of nitazoxanide in solution Drug Analytical Research |
| title | pH effect on stability and kinetics degradation of nitazoxanide in solution |
| title_full | pH effect on stability and kinetics degradation of nitazoxanide in solution |
| title_fullStr | pH effect on stability and kinetics degradation of nitazoxanide in solution |
| title_full_unstemmed | pH effect on stability and kinetics degradation of nitazoxanide in solution |
| title_short | pH effect on stability and kinetics degradation of nitazoxanide in solution |
| title_sort | ph effect on stability and kinetics degradation of nitazoxanide in solution |
| url | https://seer.ufrgs.br/index.php/poled/management/settings/website/index.php/dar/article/view/101465 |
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