Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon
The present report illustrates the response to osmotic stress of an extreme halophilic archaeon, Halorubrum sp., isolated from the saltern ponds of Margherita di Savoia in southern Italy. The hypotonic stress induces relevant changes in the membrane lipid composition: archaeal cardiolipin content ma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2004-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520319362 |
| _version_ | 1818671512177082368 |
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| author | Patrizia Lopalco Simona Lobasso Francesco Babudri Angela Corcelli |
| author_facet | Patrizia Lopalco Simona Lobasso Francesco Babudri Angela Corcelli |
| author_sort | Patrizia Lopalco |
| collection | DOAJ |
| description | The present report illustrates the response to osmotic stress of an extreme halophilic archaeon, Halorubrum sp., isolated from the saltern ponds of Margherita di Savoia in southern Italy. The hypotonic stress induces relevant changes in the membrane lipid composition: archaeal cardiolipin content markedly increases, whereas phosphatidylglycerol (PG) decreases. Membranes isolated from this archaeon after cell disruption by osmotic shock are highly enriched in archaeal cardiolipin and reveal the presence of a novel phospholipid. Electrospray ionization mass spectrometry and NMR analyses revealed that this novel lipid has the structure of a sulfo-diglyco-diether-phosphatidic acid, i.e., a phospholipid dimer or a novel cardiolipin analogue. As NMR analyses showed that the sugars in the novel phospholipid dimer are the same and in the same order of a sulfated diglycosyl diphytanylglycerol diether (S-DGD-5) present as a major lipid component in the archaeon membranes, the novel phospholipid dimer was named S-DGD-5-PA.We conclude that osmotic shock induces a specific increase in the membrane content of the two cardiolipins and suggest that PG and S-DGD-5 are intermediates for the de novo synthesis of archaeal cardiolipin and S-DGD-5-PA, respectively. |
| first_indexed | 2024-12-17T07:25:11Z |
| format | Article |
| id | doaj.art-e1885b80a550440296b5b37a2f35b2e3 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T07:25:11Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e1885b80a550440296b5b37a2f35b2e32022-12-21T21:58:40ZengElsevierJournal of Lipid Research0022-22752004-01-01451194201Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeonPatrizia Lopalco0Simona Lobasso1Francesco Babudri2Angela Corcelli3Dipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Bari, Italy; Dipartimento di Chimica, Università di Bari, Bari, ItalyDipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Bari, Italy; Dipartimento di Chimica, Università di Bari, Bari, ItalyDipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Bari, Italy; Dipartimento di Chimica, Università di Bari, Bari, ItalyDipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Bari, Italy; Dipartimento di Chimica, Università di Bari, Bari, ItalyThe present report illustrates the response to osmotic stress of an extreme halophilic archaeon, Halorubrum sp., isolated from the saltern ponds of Margherita di Savoia in southern Italy. The hypotonic stress induces relevant changes in the membrane lipid composition: archaeal cardiolipin content markedly increases, whereas phosphatidylglycerol (PG) decreases. Membranes isolated from this archaeon after cell disruption by osmotic shock are highly enriched in archaeal cardiolipin and reveal the presence of a novel phospholipid. Electrospray ionization mass spectrometry and NMR analyses revealed that this novel lipid has the structure of a sulfo-diglyco-diether-phosphatidic acid, i.e., a phospholipid dimer or a novel cardiolipin analogue. As NMR analyses showed that the sugars in the novel phospholipid dimer are the same and in the same order of a sulfated diglycosyl diphytanylglycerol diether (S-DGD-5) present as a major lipid component in the archaeon membranes, the novel phospholipid dimer was named S-DGD-5-PA.We conclude that osmotic shock induces a specific increase in the membrane content of the two cardiolipins and suggest that PG and S-DGD-5 are intermediates for the de novo synthesis of archaeal cardiolipin and S-DGD-5-PA, respectively.http://www.sciencedirect.com/science/article/pii/S0022227520319362archaeal cardiolipinsulfated diglycosyl diphytanylglycerol diether phosphatidic acidhalophilesARCHAEA |
| spellingShingle | Patrizia Lopalco Simona Lobasso Francesco Babudri Angela Corcelli Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon Journal of Lipid Research archaeal cardiolipin sulfated diglycosyl diphytanylglycerol diether phosphatidic acid halophiles ARCHAEA |
| title | Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon |
| title_full | Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon |
| title_fullStr | Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon |
| title_full_unstemmed | Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon |
| title_short | Osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon |
| title_sort | osmotic shock stimulates de novo synthesis of two cardiolipins in an extreme halophilic archaeon |
| topic | archaeal cardiolipin sulfated diglycosyl diphytanylglycerol diether phosphatidic acid halophiles ARCHAEA |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520319362 |
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