Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat
To assess the reliability and sensitivity of non-invasive optical methods to detect the early effects of water deficit in the field, we analyzed the time-series of non-invasive measurements obtained in a dry season in a representative collection of wheat genotypes grown in small-plot field trials, i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/9/1275 |
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| author | Lenka Botyanszka Marek Zivcak Erik Chovancek Oksana Sytar Viliam Barek Pavol Hauptvogel Andrej Halabuk Marian Brestic |
| author_facet | Lenka Botyanszka Marek Zivcak Erik Chovancek Oksana Sytar Viliam Barek Pavol Hauptvogel Andrej Halabuk Marian Brestic |
| author_sort | Lenka Botyanszka |
| collection | DOAJ |
| description | To assess the reliability and sensitivity of non-invasive optical methods to detect the early effects of water deficit in the field, we analyzed the time-series of non-invasive measurements obtained in a dry season in a representative collection of wheat genotypes grown in small-plot field trials, in non-irrigated and irrigated variants. Despite a progressive water deficit and significant yield loss, the measurements indicated very minor changes in chlorophyll content or canopy cover. This corresponded well to the insignificant differences in spectral reflectance normalized difference vegetation index (NDVI) values. On the other hand, we identified the significant and rapid response of fast fluorescence kinetics data following the onset of irrigation. Analysis of parameters showed the main effects of drought were associated with changes in the amplitude of the I–P phase of the OJIP transient, indicating changes at the level of photosystem I and beyond. Statistical analyses identified the integrative parameter performance index PI<sub>tot</sub> as the most sensitive parameter, which well-reflects the differences in responses of the genotypes to water deficit. Our results suggest that focusing on photosynthetic functions detected by the rapid chlorophyll fluorescence records can provide more accurate information on the drought stress level, compared to the structural data obtained by absorbance or reflectance measurements. |
| first_indexed | 2024-03-10T16:45:21Z |
| format | Article |
| id | doaj.art-e1890279416249a0bbf9d9f4641759dd |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T16:45:21Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-e1890279416249a0bbf9d9f4641759dd2023-11-20T11:43:35ZengMDPI AGAgronomy2073-43952020-08-01109127510.3390/agronomy10091275Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown WheatLenka Botyanszka0Marek Zivcak1Erik Chovancek2Oksana Sytar3Viliam Barek4Pavol Hauptvogel5Andrej Halabuk6Marian Brestic7Department of Plant Physiology, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 94901 Nitra, SlovakiaDepartment of Plant Physiology, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 94901 Nitra, SlovakiaDepartment of Plant Physiology, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 94901 Nitra, SlovakiaDepartment of Plant Physiology, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 94901 Nitra, SlovakiaDepartment of Water Resources and Environmental Engineering, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 94901 Nitra, SlovakiaNational Agricultural and Food Centre, Research Institute of Plant Production, 921 68 Piešťany, SlovakiaInstitute of Landscape Ecology, Slovak Academy of Sciences, 814 99 Bratislava, SlovakiaDepartment of Plant Physiology, Slovak University of Agriculture, Nitra, Tr. A. Hlinku 2, 94901 Nitra, SlovakiaTo assess the reliability and sensitivity of non-invasive optical methods to detect the early effects of water deficit in the field, we analyzed the time-series of non-invasive measurements obtained in a dry season in a representative collection of wheat genotypes grown in small-plot field trials, in non-irrigated and irrigated variants. Despite a progressive water deficit and significant yield loss, the measurements indicated very minor changes in chlorophyll content or canopy cover. This corresponded well to the insignificant differences in spectral reflectance normalized difference vegetation index (NDVI) values. On the other hand, we identified the significant and rapid response of fast fluorescence kinetics data following the onset of irrigation. Analysis of parameters showed the main effects of drought were associated with changes in the amplitude of the I–P phase of the OJIP transient, indicating changes at the level of photosystem I and beyond. Statistical analyses identified the integrative parameter performance index PI<sub>tot</sub> as the most sensitive parameter, which well-reflects the differences in responses of the genotypes to water deficit. Our results suggest that focusing on photosynthetic functions detected by the rapid chlorophyll fluorescence records can provide more accurate information on the drought stress level, compared to the structural data obtained by absorbance or reflectance measurements.https://www.mdpi.com/2073-4395/10/9/1275chlorophyll fluorescencespectral reflectancedroughtstressphenotypingwheat |
| spellingShingle | Lenka Botyanszka Marek Zivcak Erik Chovancek Oksana Sytar Viliam Barek Pavol Hauptvogel Andrej Halabuk Marian Brestic Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat Agronomy chlorophyll fluorescence spectral reflectance drought stress phenotyping wheat |
| title | Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat |
| title_full | Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat |
| title_fullStr | Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat |
| title_full_unstemmed | Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat |
| title_short | Chlorophyll Fluorescence Kinetics May Be Useful to Identify Early Drought and Irrigation Effects on Photosynthetic Apparatus in Field-Grown Wheat |
| title_sort | chlorophyll fluorescence kinetics may be useful to identify early drought and irrigation effects on photosynthetic apparatus in field grown wheat |
| topic | chlorophyll fluorescence spectral reflectance drought stress phenotyping wheat |
| url | https://www.mdpi.com/2073-4395/10/9/1275 |
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