Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022
Background and Aim: Foot-and-mouth disease (FMD) virus causes continuous outbreaks, leading to serious economic consequences that affect animal productivity and restrict trade movement. The potential influence of the disease was due to the emergence of new strains or re-emergence of local strains wi...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2023-07-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/July-2023/8.pdf |
| _version_ | 1797755448095408128 |
|---|---|
| author | Momtaz A. Shahein Heba A. Hussein M. H. Ali Shimaa M. Ghoniem Omayma A. Shemies Ahmed F. Afify Amthal Ahmed Fuoad Ayah M. Hassan Mostafa R. Zaher Nahla Hussien AbouEl Ela Ahmed R. Habashi Samah Eid Naglaa M. Hagag |
| author_facet | Momtaz A. Shahein Heba A. Hussein M. H. Ali Shimaa M. Ghoniem Omayma A. Shemies Ahmed F. Afify Amthal Ahmed Fuoad Ayah M. Hassan Mostafa R. Zaher Nahla Hussien AbouEl Ela Ahmed R. Habashi Samah Eid Naglaa M. Hagag |
| author_sort | Momtaz A. Shahein |
| collection | DOAJ |
| description | Background and Aim: Foot-and-mouth disease (FMD) virus causes continuous outbreaks, leading to serious economic consequences that affect animal productivity and restrict trade movement. The potential influence of the disease was due to the emergence of new strains or re-emergence of local strains with major antigenic variations due to genetic mutations. This study aims to evaluate circulating virus in samples collected from infected animals during an outbreak using antigenic characterization and identify whether there is an emergence of a new strain or mutation.
Materials and Methods: Reverse-transcription polymerase chain reaction (RT-PCR) was used to screen 86 samples. Viral protein 1 (VP1) codon sequencing was performed. The virus was isolated from the samples inoculated on the baby-hamster kidney cell line and Enzyme-linked immunosorbent assay was performed for serotyping and antigen detection.
Results: Based on the RT-PCR screening results, 10 positive samples were selected for sequencing. The sequences belonged to the FMD serotype A African topotype originating from the ancestor prototype Sudan/77, with which it shared 98.48% ± 1.2% similarity. The divergence with local isolates from 2020 was 9.3%. In addition, the sequences were 96.84% ± 1.01% and 95.84% ± 0.79% related to Egyptian-Damietta type 2016 and Sudanese-2018, respectively. Divergence with vaccinal strains ranged from 10% to 17%. Amino acid sequence analysis revealed that the isolates had variation in the most prominent antigenic regions (residues 35–75) and the immunogenic determinants of the G-H loop of VP1 (residues 100–146 and 161–175).
Conclusion: The current isolates should be included in the locally produced vaccine to provide broader immunogenic coverage against serotype A African topotypes. |
| first_indexed | 2024-03-12T17:48:01Z |
| format | Article |
| id | doaj.art-e18a00ba94f9453cb77a3021a8060246 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-12T17:48:01Z |
| publishDate | 2023-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-e18a00ba94f9453cb77a3021a80602462023-08-03T13:20:55ZengVeterinary WorldVeterinary World0972-89882231-09162023-07-011671429143710.14202/vetworld.2023.1429-1437Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022Momtaz A. Shahein0Heba A. Hussein1https://orcid.org/0000-0003-1058-4946M. H. Ali2https://orcid.org/0000-0003-1560-3632Shimaa M. Ghoniem3Omayma A. Shemies4Ahmed F. Afify5https://orcid.org/0000-0002-7620-9084Amthal Ahmed Fuoad6Ayah M. Hassan7https://orcid.org/0000-0003-4202-6025Mostafa R. Zaher8https://orcid.org/0000-0002-8267-2702Nahla Hussien AbouEl Ela9https://orcid.org/0000-0002-0408-864XAhmed R. Habashi10https://orcid.org/0000-0002-7379-4468Samah Eid11Naglaa M. Hagag12https://orcid.org/0000-0002-2598-3264Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Department of Virology, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Genome Research Unit, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Genome Research Unit, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Genome Research Unit, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Virus Strain Bank, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Genome Research Unit, Animal Health Research Institute, Agricultural Research Center, Giza, 12619, Egypt.Background and Aim: Foot-and-mouth disease (FMD) virus causes continuous outbreaks, leading to serious economic consequences that affect animal productivity and restrict trade movement. The potential influence of the disease was due to the emergence of new strains or re-emergence of local strains with major antigenic variations due to genetic mutations. This study aims to evaluate circulating virus in samples collected from infected animals during an outbreak using antigenic characterization and identify whether there is an emergence of a new strain or mutation. Materials and Methods: Reverse-transcription polymerase chain reaction (RT-PCR) was used to screen 86 samples. Viral protein 1 (VP1) codon sequencing was performed. The virus was isolated from the samples inoculated on the baby-hamster kidney cell line and Enzyme-linked immunosorbent assay was performed for serotyping and antigen detection. Results: Based on the RT-PCR screening results, 10 positive samples were selected for sequencing. The sequences belonged to the FMD serotype A African topotype originating from the ancestor prototype Sudan/77, with which it shared 98.48% ± 1.2% similarity. The divergence with local isolates from 2020 was 9.3%. In addition, the sequences were 96.84% ± 1.01% and 95.84% ± 0.79% related to Egyptian-Damietta type 2016 and Sudanese-2018, respectively. Divergence with vaccinal strains ranged from 10% to 17%. Amino acid sequence analysis revealed that the isolates had variation in the most prominent antigenic regions (residues 35–75) and the immunogenic determinants of the G-H loop of VP1 (residues 100–146 and 161–175). Conclusion: The current isolates should be included in the locally produced vaccine to provide broader immunogenic coverage against serotype A African topotypes.https://www.veterinaryworld.org/Vol.16/July-2023/8.pdfepicentersfoot and mouth disease virusfoot-and-mouth disease virus africa type g-ivmutationsoutbreaksviral protein 1 |
| spellingShingle | Momtaz A. Shahein Heba A. Hussein M. H. Ali Shimaa M. Ghoniem Omayma A. Shemies Ahmed F. Afify Amthal Ahmed Fuoad Ayah M. Hassan Mostafa R. Zaher Nahla Hussien AbouEl Ela Ahmed R. Habashi Samah Eid Naglaa M. Hagag Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 Veterinary World epicenters foot and mouth disease virus foot-and-mouth disease virus africa type g-iv mutations outbreaks viral protein 1 |
| title | Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 |
| title_full | Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 |
| title_fullStr | Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 |
| title_full_unstemmed | Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 |
| title_short | Circulating foot-and-mouth disease virus serotype A African-genotype IV in Egypt during 2022 |
| title_sort | circulating foot and mouth disease virus serotype a african genotype iv in egypt during 2022 |
| topic | epicenters foot and mouth disease virus foot-and-mouth disease virus africa type g-iv mutations outbreaks viral protein 1 |
| url | https://www.veterinaryworld.org/Vol.16/July-2023/8.pdf |
| work_keys_str_mv | AT momtazashahein circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT hebaahussein circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT mhali circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT shimaamghoniem circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT omaymaashemies circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT ahmedfafify circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT amthalahmedfuoad circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT ayahmhassan circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT mostafarzaher circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT nahlahussienabouelela circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT ahmedrhabashi circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT samaheid circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 AT naglaamhagag circulatingfootandmouthdiseasevirusserotypeaafricangenotypeivinegyptduring2022 |