Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota
Polysaccharides from the species of Boletaceae (Boletales, Agaricomycetes, Basidiomycota) are economically significant to both functional foods and medicinal industries. The crude polysaccharide from <i>Butyriboletus pseudospeciosus</i> (BPP) was prepared, and its physicochemical propert...
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/6/2679 |
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| author | Run Tian Lu-Ling Wu Hong-Fu Li Zhi-Qun Liang Pei-Hu Li Yong Wang Nian-Kai Zeng |
| author_facet | Run Tian Lu-Ling Wu Hong-Fu Li Zhi-Qun Liang Pei-Hu Li Yong Wang Nian-Kai Zeng |
| author_sort | Run Tian |
| collection | DOAJ |
| description | Polysaccharides from the species of Boletaceae (Boletales, Agaricomycetes, Basidiomycota) are economically significant to both functional foods and medicinal industries. The crude polysaccharide from <i>Butyriboletus pseudospeciosus</i> (BPP) was prepared, and its physicochemical properties were characterized through the use of consecutive experimental apparatus, and its impact on the gut microbiota of Kunming mice was evaluated. Analyses of the structure characteristics revealed that BPP was mainly composed of Man, Glc, and Gal, possessing the pyranose ring and β/α-glycosidic linkages. TG analysis exhibited that BPP had great heat stability. The SEM observation performed demonstrated that BPP appeared with a rough, dense, and porous shape. Through the BPP intervention, the serum and fecal biochemical index in mice can be improved obviously (<i>p</i> < 0.05). The abundance of beneficial microbiota in the BPP-treated group was significantly increased, while the abundance of harmful microbiota was significantly decreased (<i>p</i> < 0.05). Based on the Tax4Fun, we also revealed the relationship between the species of gut microbiota and showed that the high dose of BPP has significantly changed the functional diversities compared with those in other groups (<i>p</i> < 0.05). The results suggest that <i>B. pseudospeciosus</i> could serve as potential functional food or medicine. |
| first_indexed | 2024-03-11T06:07:52Z |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T06:07:52Z |
| publishDate | 2023-03-01 |
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| series | Molecules |
| spelling | doaj.art-e18e887410c146e09020060ab37942f22023-11-17T12:53:43ZengMDPI AGMolecules1420-30492023-03-01286267910.3390/molecules28062679Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut MicrobiotaRun Tian0Lu-Ling Wu1Hong-Fu Li2Zhi-Qun Liang3Pei-Hu Li4Yong Wang5Nian-Kai Zeng6Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Pharmacy, School of Tropical Medicine, College of Biomedical Information and Engineering, Hainan Medical University, Haikou 571199, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Pharmacy, School of Tropical Medicine, College of Biomedical Information and Engineering, Hainan Medical University, Haikou 571199, ChinaSchool of Pharmacy, North China University of Science and Technology, Tangshan 063210, ChinaCollege of Science, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Pharmacy, School of Tropical Medicine, College of Biomedical Information and Engineering, Hainan Medical University, Haikou 571199, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Pharmacy, School of Tropical Medicine, College of Biomedical Information and Engineering, Hainan Medical University, Haikou 571199, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Pharmacy, School of Tropical Medicine, College of Biomedical Information and Engineering, Hainan Medical University, Haikou 571199, ChinaPolysaccharides from the species of Boletaceae (Boletales, Agaricomycetes, Basidiomycota) are economically significant to both functional foods and medicinal industries. The crude polysaccharide from <i>Butyriboletus pseudospeciosus</i> (BPP) was prepared, and its physicochemical properties were characterized through the use of consecutive experimental apparatus, and its impact on the gut microbiota of Kunming mice was evaluated. Analyses of the structure characteristics revealed that BPP was mainly composed of Man, Glc, and Gal, possessing the pyranose ring and β/α-glycosidic linkages. TG analysis exhibited that BPP had great heat stability. The SEM observation performed demonstrated that BPP appeared with a rough, dense, and porous shape. Through the BPP intervention, the serum and fecal biochemical index in mice can be improved obviously (<i>p</i> < 0.05). The abundance of beneficial microbiota in the BPP-treated group was significantly increased, while the abundance of harmful microbiota was significantly decreased (<i>p</i> < 0.05). Based on the Tax4Fun, we also revealed the relationship between the species of gut microbiota and showed that the high dose of BPP has significantly changed the functional diversities compared with those in other groups (<i>p</i> < 0.05). The results suggest that <i>B. pseudospeciosus</i> could serve as potential functional food or medicine.https://www.mdpi.com/1420-3049/28/6/2679crude polysaccharide from <i>Butyriboletus pseudospeciosus</i> (BPP)physicochemical propertiesfunctionsgut microbiotaTax4Fun |
| spellingShingle | Run Tian Lu-Ling Wu Hong-Fu Li Zhi-Qun Liang Pei-Hu Li Yong Wang Nian-Kai Zeng Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota Molecules crude polysaccharide from <i>Butyriboletus pseudospeciosus</i> (BPP) physicochemical properties functions gut microbiota Tax4Fun |
| title | Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota |
| title_full | Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota |
| title_fullStr | Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota |
| title_full_unstemmed | Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota |
| title_short | Purification and Structure Characterization of the Crude Polysaccharide from the Fruiting Bodies of <i>Butyriboletus pseudospeciosus</i> and Its Modulation Effects on Gut Microbiota |
| title_sort | purification and structure characterization of the crude polysaccharide from the fruiting bodies of i butyriboletus pseudospeciosus i and its modulation effects on gut microbiota |
| topic | crude polysaccharide from <i>Butyriboletus pseudospeciosus</i> (BPP) physicochemical properties functions gut microbiota Tax4Fun |
| url | https://www.mdpi.com/1420-3049/28/6/2679 |
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