Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol
Objective: To study peculiarities of skin wound healing under ischemic conditions with topical treatment using a combination of benzalkonium chloride and dexpanthenol.Materials and methods: We conducted an experiment on a rat model of skin wound healing under ischemic conditions. Male Wistar rats we...
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| Format: | Article |
| Language: | Russian |
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Scientific Research Institute, Ochapovsky Regional Clinical Hospital no. 1
2024-03-01
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| Series: | Инновационная медицина Кубани |
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| Online Access: | https://www.innovmedkub.ru/jour/article/view/820 |
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| author | A. G. Terekhov E. S. Mishina E. G. Klyueva P. A. Bukanova D. Ya. Khojiev A. Yu. Grigoryan |
| author_facet | A. G. Terekhov E. S. Mishina E. G. Klyueva P. A. Bukanova D. Ya. Khojiev A. Yu. Grigoryan |
| author_sort | A. G. Terekhov |
| collection | DOAJ |
| description | Objective: To study peculiarities of skin wound healing under ischemic conditions with topical treatment using a combination of benzalkonium chloride and dexpanthenol.Materials and methods: We conducted an experiment on a rat model of skin wound healing under ischemic conditions. Male Wistar rats were divided into 4 groups, with 30 rats in each group. Group 1 received no treatment; group 2 was treated with the Levomecol ointment; group 3 and group 4 were treated with benzalkonium chloride immobilized based on the carboxymethylcellulose sodium salt and a combination of benzalkonium chloride and dexpanthenol immobilized based on the carboxymethylcellulose sodium salt, respectively. We used planimetric and biochemical (alkaline phosphatase [ALP] level) methods, measured the pH of the wound surface and wound bed temperature, determined the hydroxyproline concentration in the wound defect tissues, and performed statistical processing of the data.Results: Group 4 had the largest percentage of wound surface area reduction and pH values. Thermometry on day 10 showed a decrease in wound temperature in groups 2 and 4, whereas groups 1 and 3 demonstrated maximum values. By the end of the experiment, group 4 had the maximum hydroxyproline concentration that was significantly higher than the amino acid content in groups 1, 2, and 3: 1.2, 1.1 and 1.1 times higher, respectively. Maximum ALP levels were observed on day 5 in group 4, whereas in groups 2 and 3 they were observed on day 8 and only on day 10 in group 1.Conclusions: Skin wound healing under ischemic conditions was faster in the group in which topical treatment involved a combination developed by us: benzalkonium chloride and dexpanthenol immobilized based on the carboxymethylcellulose sodium salt. |
| first_indexed | 2024-03-07T15:35:16Z |
| format | Article |
| id | doaj.art-e18e94ca64554b469a8d26530f14159e |
| institution | Directory Open Access Journal |
| issn | 2541-9897 |
| language | Russian |
| last_indexed | 2024-03-07T15:35:16Z |
| publishDate | 2024-03-01 |
| publisher | Scientific Research Institute, Ochapovsky Regional Clinical Hospital no. 1 |
| record_format | Article |
| series | Инновационная медицина Кубани |
| spelling | doaj.art-e18e94ca64554b469a8d26530f14159e2024-03-05T11:12:33ZrusScientific Research Institute, Ochapovsky Regional Clinical Hospital no. 1Инновационная медицина Кубани2541-98972024-03-010111312110.35401/2541-9897-2024-9-1-113-121434Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and DexpanthenolA. G. Terekhov0E. S. Mishina1E. G. Klyueva2P. A. Bukanova3D. Ya. Khojiev4A. Yu. Grigoryan5Kursk State Medical UniversityKursk State Medical UniversityKursk State Medical UniversityKursk State Medical UniversityTermez Branch of the Tashkent Medical AcademyKursk State Medical UniversityObjective: To study peculiarities of skin wound healing under ischemic conditions with topical treatment using a combination of benzalkonium chloride and dexpanthenol.Materials and methods: We conducted an experiment on a rat model of skin wound healing under ischemic conditions. Male Wistar rats were divided into 4 groups, with 30 rats in each group. Group 1 received no treatment; group 2 was treated with the Levomecol ointment; group 3 and group 4 were treated with benzalkonium chloride immobilized based on the carboxymethylcellulose sodium salt and a combination of benzalkonium chloride and dexpanthenol immobilized based on the carboxymethylcellulose sodium salt, respectively. We used planimetric and biochemical (alkaline phosphatase [ALP] level) methods, measured the pH of the wound surface and wound bed temperature, determined the hydroxyproline concentration in the wound defect tissues, and performed statistical processing of the data.Results: Group 4 had the largest percentage of wound surface area reduction and pH values. Thermometry on day 10 showed a decrease in wound temperature in groups 2 and 4, whereas groups 1 and 3 demonstrated maximum values. By the end of the experiment, group 4 had the maximum hydroxyproline concentration that was significantly higher than the amino acid content in groups 1, 2, and 3: 1.2, 1.1 and 1.1 times higher, respectively. Maximum ALP levels were observed on day 5 in group 4, whereas in groups 2 and 3 they were observed on day 8 and only on day 10 in group 1.Conclusions: Skin wound healing under ischemic conditions was faster in the group in which topical treatment involved a combination developed by us: benzalkonium chloride and dexpanthenol immobilized based on the carboxymethylcellulose sodium salt.https://www.innovmedkub.ru/jour/article/view/820topical wound treatmentwound healingischemic conditionsdexpanthenolbenzalkonium chloride |
| spellingShingle | A. G. Terekhov E. S. Mishina E. G. Klyueva P. A. Bukanova D. Ya. Khojiev A. Yu. Grigoryan Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol Инновационная медицина Кубани topical wound treatment wound healing ischemic conditions dexpanthenol benzalkonium chloride |
| title | Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol |
| title_full | Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol |
| title_fullStr | Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol |
| title_full_unstemmed | Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol |
| title_short | Peculiarities of Skin Wound healing Under Ischemic Conditions With Topical Treatment Using a Combination of Benzalkonium Chloride and Dexpanthenol |
| title_sort | peculiarities of skin wound healing under ischemic conditions with topical treatment using a combination of benzalkonium chloride and dexpanthenol |
| topic | topical wound treatment wound healing ischemic conditions dexpanthenol benzalkonium chloride |
| url | https://www.innovmedkub.ru/jour/article/view/820 |
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