Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein
Background: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retin...
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MDPI AG
2022-06-01
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| author | Katharina Görtemaker Chad Yee Rabea Bartölke Heide Behrmann Jan-Oliver Voß Jessica Schmidt Jingjing Xu Vita Solovyeva Bo Leberecht Elmar Behrmann Henrik Mouritsen Karl-Wilhelm Koch |
| author_facet | Katharina Görtemaker Chad Yee Rabea Bartölke Heide Behrmann Jan-Oliver Voß Jessica Schmidt Jingjing Xu Vita Solovyeva Bo Leberecht Elmar Behrmann Henrik Mouritsen Karl-Wilhelm Koch |
| author_sort | Katharina Görtemaker |
| collection | DOAJ |
| description | Background: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein–protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception. |
| first_indexed | 2024-03-09T22:02:03Z |
| format | Article |
| id | doaj.art-e18ee1cb8df64c8ba45b338fca3d87b5 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T22:02:03Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e18ee1cb8df64c8ba45b338fca3d87b52023-11-23T19:48:33ZengMDPI AGCells2073-44092022-06-011113204310.3390/cells11132043Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-ProteinKatharina Görtemaker0Chad Yee1Rabea Bartölke2Heide Behrmann3Jan-Oliver Voß4Jessica Schmidt5Jingjing Xu6Vita Solovyeva7Bo Leberecht8Elmar Behrmann9Henrik Mouritsen10Karl-Wilhelm Koch11Division of Biochemistry, Department of Neuroscience, University of Oldenburg, D-26111 Oldenburg, GermanyDivision of Biochemistry, Department of Neuroscience, University of Oldenburg, D-26111 Oldenburg, GermanyAnimal Navigation, Institute of Biology and Environmental Sciences, University of Oldenburg, D-26111 Oldenburg, GermanyInstitute of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Cologne, D-50674 Cologne, GermanyDivision of Biochemistry, Department of Neuroscience, University of Oldenburg, D-26111 Oldenburg, GermanyAnimal Navigation, Institute of Biology and Environmental Sciences, University of Oldenburg, D-26111 Oldenburg, GermanyAnimal Navigation, Institute of Biology and Environmental Sciences, University of Oldenburg, D-26111 Oldenburg, GermanyInstitute of Physics, University of Oldenburg, D-26111 Oldenburg, GermanyAnimal Navigation, Institute of Biology and Environmental Sciences, University of Oldenburg, D-26111 Oldenburg, GermanyInstitute of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Cologne, D-50674 Cologne, GermanyAnimal Navigation, Institute of Biology and Environmental Sciences, University of Oldenburg, D-26111 Oldenburg, GermanyDivision of Biochemistry, Department of Neuroscience, University of Oldenburg, D-26111 Oldenburg, GermanyBackground: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein–protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception.https://www.mdpi.com/2073-4409/11/13/2043magnetoreceptioncryptochromeG protein α-subunitprotein-protein interaction |
| spellingShingle | Katharina Görtemaker Chad Yee Rabea Bartölke Heide Behrmann Jan-Oliver Voß Jessica Schmidt Jingjing Xu Vita Solovyeva Bo Leberecht Elmar Behrmann Henrik Mouritsen Karl-Wilhelm Koch Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein Cells magnetoreception cryptochrome G protein α-subunit protein-protein interaction |
| title | Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein |
| title_full | Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein |
| title_fullStr | Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein |
| title_full_unstemmed | Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein |
| title_short | Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein |
| title_sort | direct interaction of avian cryptochrome 4 with a cone specific g protein |
| topic | magnetoreception cryptochrome G protein α-subunit protein-protein interaction |
| url | https://www.mdpi.com/2073-4409/11/13/2043 |
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