Unconventional meiotic process of spermatocytes in male Cyprinus carpio
The spermatogenesis of fish remained unclear about the cytological process, especially on the meiotic process of spermatocytes after meiosis I. In the present study, microstructure and ultrastructure of spermatogenesis in Cyprinus carpio (abbreviated CC) were studied, the meiosis process was traced...
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KeAi Communications Co. Ltd.
2021-03-01
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| Series: | Reproduction and Breeding |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667071221000065 |
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| author | La Zhu Wangchao He Hui Zhang Yu Sun Qi Li Luojing Zhou Shurun Zhu Min Tao Yi Zhou Rurong Zhao Kaikun Luo Chenchen Tang Chun Zhang Shaojun Liu |
| author_facet | La Zhu Wangchao He Hui Zhang Yu Sun Qi Li Luojing Zhou Shurun Zhu Min Tao Yi Zhou Rurong Zhao Kaikun Luo Chenchen Tang Chun Zhang Shaojun Liu |
| author_sort | La Zhu |
| collection | DOAJ |
| description | The spermatogenesis of fish remained unclear about the cytological process, especially on the meiotic process of spermatocytes after meiosis I. In the present study, microstructure and ultrastructure of spermatogenesis in Cyprinus carpio (abbreviated CC) were studied, the meiosis process was traced by chromosomal observation and the corresponding immunohistochemical staining of testis where spermatocytes at different stages were distinguished. Results firstly described the telomere-led bouquet configuration of prophase I-stage cells in fish, which ensure the normal chromosomal behavior just like in other eukaryotes. Compared to the canonical meiotic process, the spermatocytes at leptotene, zygotene, pachytene, diplotene and diakinesis stages in CC were normal and also proceeded metaphase I interacting with the meiotic spindle as a unit, while the subsequently meiosis II in CC demonstrated contradicted findings in most organisms, in which no obvious spindle-dependent sister chromatids aligning and segregating could be observed, but some circular condensed nucleus structures. Furthermore, protein antibody of RAD51, DMC1, RNA-Pol II CTD P–S5, H3K36me3 were used respectively as probes, marked initially between the primary spermatocytes at prophase I and metaphase I stage, and between the spermatids and spermatozoa. The systematical study in cytological characteristics of the whole meiotic process in fish spermatogenesis in this paper, provide a standard reference for fish meiosis, which can be of great significance in the practice of fish genetics and breeding. |
| first_indexed | 2024-04-10T18:29:19Z |
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| spelling | doaj.art-e18f032982654712915e72cc9a24df552023-02-02T04:50:38ZengKeAi Communications Co. Ltd.Reproduction and Breeding2667-07122021-03-01114047Unconventional meiotic process of spermatocytes in male Cyprinus carpioLa Zhu0Wangchao He1Hui Zhang2Yu Sun3Qi Li4Luojing Zhou5Shurun Zhu6Min Tao7Yi Zhou8Rurong Zhao9Kaikun Luo10Chenchen Tang11Chun Zhang12Shaojun Liu13State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaCorresponding author.; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaCorresponding author.; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha, 410081, Hunan, PR ChinaThe spermatogenesis of fish remained unclear about the cytological process, especially on the meiotic process of spermatocytes after meiosis I. In the present study, microstructure and ultrastructure of spermatogenesis in Cyprinus carpio (abbreviated CC) were studied, the meiosis process was traced by chromosomal observation and the corresponding immunohistochemical staining of testis where spermatocytes at different stages were distinguished. Results firstly described the telomere-led bouquet configuration of prophase I-stage cells in fish, which ensure the normal chromosomal behavior just like in other eukaryotes. Compared to the canonical meiotic process, the spermatocytes at leptotene, zygotene, pachytene, diplotene and diakinesis stages in CC were normal and also proceeded metaphase I interacting with the meiotic spindle as a unit, while the subsequently meiosis II in CC demonstrated contradicted findings in most organisms, in which no obvious spindle-dependent sister chromatids aligning and segregating could be observed, but some circular condensed nucleus structures. Furthermore, protein antibody of RAD51, DMC1, RNA-Pol II CTD P–S5, H3K36me3 were used respectively as probes, marked initially between the primary spermatocytes at prophase I and metaphase I stage, and between the spermatids and spermatozoa. The systematical study in cytological characteristics of the whole meiotic process in fish spermatogenesis in this paper, provide a standard reference for fish meiosis, which can be of great significance in the practice of fish genetics and breeding.http://www.sciencedirect.com/science/article/pii/S2667071221000065SpermatogenesisMeiosisKaryopyknosisSpindleThe secondary spermatocyteCircular condensed nucleus |
| spellingShingle | La Zhu Wangchao He Hui Zhang Yu Sun Qi Li Luojing Zhou Shurun Zhu Min Tao Yi Zhou Rurong Zhao Kaikun Luo Chenchen Tang Chun Zhang Shaojun Liu Unconventional meiotic process of spermatocytes in male Cyprinus carpio Reproduction and Breeding Spermatogenesis Meiosis Karyopyknosis Spindle The secondary spermatocyte Circular condensed nucleus |
| title | Unconventional meiotic process of spermatocytes in male Cyprinus carpio |
| title_full | Unconventional meiotic process of spermatocytes in male Cyprinus carpio |
| title_fullStr | Unconventional meiotic process of spermatocytes in male Cyprinus carpio |
| title_full_unstemmed | Unconventional meiotic process of spermatocytes in male Cyprinus carpio |
| title_short | Unconventional meiotic process of spermatocytes in male Cyprinus carpio |
| title_sort | unconventional meiotic process of spermatocytes in male cyprinus carpio |
| topic | Spermatogenesis Meiosis Karyopyknosis Spindle The secondary spermatocyte Circular condensed nucleus |
| url | http://www.sciencedirect.com/science/article/pii/S2667071221000065 |
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