Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program
Tench is a cyprinid fish that has undergone human-induced translocations. The natural populations of the species are on the decline due to habitat loss and spawning grounds degradation. The genetic diversity of seven natural populations was investigated to establish the genetic knowledge base for su...
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MDPI AG
2022-04-01
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| author | Fatema Ali Al Fatle Erika Edviné Meleg Zoltán Sallai Gergely Szabó Eszter Várkonyi Béla Urbányi Balázs Kovács Tamás Molnár István Lehoczky |
| author_facet | Fatema Ali Al Fatle Erika Edviné Meleg Zoltán Sallai Gergely Szabó Eszter Várkonyi Béla Urbányi Balázs Kovács Tamás Molnár István Lehoczky |
| author_sort | Fatema Ali Al Fatle |
| collection | DOAJ |
| description | Tench is a cyprinid fish that has undergone human-induced translocations. The natural populations of the species are on the decline due to habitat loss and spawning grounds degradation. The genetic diversity of seven natural populations was investigated to establish the genetic knowledge base for successful conservation efforts and for selective breeding. Twelve microsatellite markers, the sequencing of a 615 bp section of mtDNA (<i>Cytb</i>) and PCR-RFLP analysis of two nuclear markers (<i>Act</i>) and (<i>RpS7</i>) were used to analyze the genetic variation and structure among 175 individuals. All microsatellite loci were found to have moderate levels of polymorphism. The pairwise Fst values between population pairings were moderate; the populations were aligned to four clusters. The <i>Cytb</i> gene showed 20 haplotypes; 67.1% of individuals were categorized as Eastern, while 32.9% to the Western haplogroup. Analysis of the <i>Act</i> and <i>RpS7</i> genes showed that the level of hybridization among the two haplogroups is high within the sampled populations. Hungarian Tench populations are genetically less diverse compared to natural populations in Western-Europe, but they still represent valuable genetic resources and Lake Fertő, Lake Kolon and Csörnöc-Herpenyő populations can be optimal candidates for future selective breeding programs. |
| first_indexed | 2024-03-10T03:02:23Z |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T03:02:23Z |
| publishDate | 2022-04-01 |
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| record_format | Article |
| series | Diversity |
| spelling | doaj.art-e1902ac833b241f1897768eb561d21862023-11-23T10:42:41ZengMDPI AGDiversity1424-28182022-04-0114533610.3390/d14050336Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding ProgramFatema Ali Al Fatle0Erika Edviné Meleg1Zoltán Sallai2Gergely Szabó3Eszter Várkonyi4Béla Urbányi5Balázs Kovács6Tamás Molnár7István Lehoczky8Doctoral School of Biological Sciences, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 2100 Gödöllő, HungaryVaskos Csabak Ltd., 5540 Szarvas, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 2100 Gödöllő, HungaryInstitute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryInstitute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 2100 Gödöllő, HungaryNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, 2100 Gödöllő, HungaryTench is a cyprinid fish that has undergone human-induced translocations. The natural populations of the species are on the decline due to habitat loss and spawning grounds degradation. The genetic diversity of seven natural populations was investigated to establish the genetic knowledge base for successful conservation efforts and for selective breeding. Twelve microsatellite markers, the sequencing of a 615 bp section of mtDNA (<i>Cytb</i>) and PCR-RFLP analysis of two nuclear markers (<i>Act</i>) and (<i>RpS7</i>) were used to analyze the genetic variation and structure among 175 individuals. All microsatellite loci were found to have moderate levels of polymorphism. The pairwise Fst values between population pairings were moderate; the populations were aligned to four clusters. The <i>Cytb</i> gene showed 20 haplotypes; 67.1% of individuals were categorized as Eastern, while 32.9% to the Western haplogroup. Analysis of the <i>Act</i> and <i>RpS7</i> genes showed that the level of hybridization among the two haplogroups is high within the sampled populations. Hungarian Tench populations are genetically less diverse compared to natural populations in Western-Europe, but they still represent valuable genetic resources and Lake Fertő, Lake Kolon and Csörnöc-Herpenyő populations can be optimal candidates for future selective breeding programs.https://www.mdpi.com/1424-2818/14/5/336Tenchmicrosatellite<i>Cytb</i> haplotype networkhybrid zonediversity |
| spellingShingle | Fatema Ali Al Fatle Erika Edviné Meleg Zoltán Sallai Gergely Szabó Eszter Várkonyi Béla Urbányi Balázs Kovács Tamás Molnár István Lehoczky Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program Diversity Tench microsatellite <i>Cytb</i> haplotype network hybrid zone diversity |
| title | Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program |
| title_full | Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program |
| title_fullStr | Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program |
| title_full_unstemmed | Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program |
| title_short | Genetic Structure and Diversity of Native Tench (<i>Tinca tinca</i> L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program |
| title_sort | genetic structure and diversity of native tench i tinca tinca i l 1758 populations in hungary establishment of basic knowledge base for a breeding program |
| topic | Tench microsatellite <i>Cytb</i> haplotype network hybrid zone diversity |
| url | https://www.mdpi.com/1424-2818/14/5/336 |
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