The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism
Intracellular parasitism often results in gene loss, genome reduction, and dependence upon the host for cellular functioning. Rozellomycota is a clade comprising many such parasites and is related to the diverse, highly reduced, animal parasites, Microsporidia. We sequenced the nuclear and mitochond...
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| Format: | Article |
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eLife Sciences Publications Ltd
2017-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/29594 |
| _version_ | 1811200601940295680 |
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| author | C Alisha Quandt Denis Beaudet Daniele Corsaro Julia Walochnik Rolf Michel Nicolas Corradi Timothy Y James |
| author_facet | C Alisha Quandt Denis Beaudet Daniele Corsaro Julia Walochnik Rolf Michel Nicolas Corradi Timothy Y James |
| author_sort | C Alisha Quandt |
| collection | DOAJ |
| description | Intracellular parasitism often results in gene loss, genome reduction, and dependence upon the host for cellular functioning. Rozellomycota is a clade comprising many such parasites and is related to the diverse, highly reduced, animal parasites, Microsporidia. We sequenced the nuclear and mitochondrial genomes of Paramicrosporidium saccamoebae [Rozellomycota], an intranuclear parasite of amoebae. A canonical fungal mitochondrial genome was recovered from P. saccamoebae that encodes genes necessary for the complete oxidative phosphorylation pathway including Complex I, differentiating it from most endoparasites including its sequenced relatives in Rozellomycota and Microsporidia. Comparative analysis revealed that P. saccamoebae shares more gene content with distantly related Fungi than with its closest relatives, suggesting that genome evolution in Rozellomycota and Microsporidia has been affected by repeated and independent gene losses, possibly as a result of variation in parasitic strategies (e.g. host and subcellular localization) or due to multiple transitions to parasitism. |
| first_indexed | 2024-04-12T02:06:18Z |
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| id | doaj.art-e193fc3d67e14b8da10e483933d0d0fc |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:06:18Z |
| publishDate | 2017-11-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj.art-e193fc3d67e14b8da10e483933d0d0fc2022-12-22T03:52:31ZengeLife Sciences Publications LtdeLife2050-084X2017-11-01610.7554/eLife.29594The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitismC Alisha Quandt0https://orcid.org/0000-0003-0260-8995Denis Beaudet1Daniele Corsaro2Julia Walochnik3Rolf Michel4Nicolas Corradi5https://orcid.org/0000-0002-7932-7932Timothy Y James6https://orcid.org/0000-0002-1123-5986Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, United StatesDepartment of Biology, University of Ottawa, Ottawa, CanadaCHLAREAS Chlamydia Research Association, Nancy, FranceMolecular Parasitology, Institute for Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Koblenz, GermanyLaboratory of Electron Microscopy, Central Institute of the Federal Armed Forces Medical Services, Koblenz, GermanyDepartment of Biology, University of Ottawa, Ottawa, CanadaDepartment of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, United StatesIntracellular parasitism often results in gene loss, genome reduction, and dependence upon the host for cellular functioning. Rozellomycota is a clade comprising many such parasites and is related to the diverse, highly reduced, animal parasites, Microsporidia. We sequenced the nuclear and mitochondrial genomes of Paramicrosporidium saccamoebae [Rozellomycota], an intranuclear parasite of amoebae. A canonical fungal mitochondrial genome was recovered from P. saccamoebae that encodes genes necessary for the complete oxidative phosphorylation pathway including Complex I, differentiating it from most endoparasites including its sequenced relatives in Rozellomycota and Microsporidia. Comparative analysis revealed that P. saccamoebae shares more gene content with distantly related Fungi than with its closest relatives, suggesting that genome evolution in Rozellomycota and Microsporidia has been affected by repeated and independent gene losses, possibly as a result of variation in parasitic strategies (e.g. host and subcellular localization) or due to multiple transitions to parasitism.https://elifesciences.org/articles/29594mitochondriagenome reductionRozellaMicrosporidiaFungiRozellomycota |
| spellingShingle | C Alisha Quandt Denis Beaudet Daniele Corsaro Julia Walochnik Rolf Michel Nicolas Corradi Timothy Y James The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism eLife mitochondria genome reduction Rozella Microsporidia Fungi Rozellomycota |
| title | The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism |
| title_full | The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism |
| title_fullStr | The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism |
| title_full_unstemmed | The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism |
| title_short | The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism |
| title_sort | genome of an intranuclear parasite paramicrosporidium saccamoebae reveals alternative adaptations to obligate intracellular parasitism |
| topic | mitochondria genome reduction Rozella Microsporidia Fungi Rozellomycota |
| url | https://elifesciences.org/articles/29594 |
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