Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria
Extended-spectrum β-lactamase (ESBL)-producing organisms have become a serious challenge in healthcare delivery globally. The prevalence of ESBL carriage in healthy and sick children in Enugu, Nigeria, was bacteriologically investigated in this study. Four hundred and twenty-two biological...
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MDPI AG
2019-11-01
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| author | Angus N. Oli Vitalis I. Ogbuagu Chika P. Ejikeugwu Ifeanyichukwu R. Iroha Malachy C. Ugwu Chijioke M. Ofomata Kenneth N. Okeke George O. Emechebe Jude C. Okoro Chukwudi O. Okani Stanley K. Onah |
| author_facet | Angus N. Oli Vitalis I. Ogbuagu Chika P. Ejikeugwu Ifeanyichukwu R. Iroha Malachy C. Ugwu Chijioke M. Ofomata Kenneth N. Okeke George O. Emechebe Jude C. Okoro Chukwudi O. Okani Stanley K. Onah |
| author_sort | Angus N. Oli |
| collection | DOAJ |
| description | Extended-spectrum β-lactamase (ESBL)-producing organisms have become a serious challenge in healthcare delivery globally. The prevalence of ESBL carriage in healthy and sick children in Enugu, Nigeria, was bacteriologically investigated in this study. Four hundred and twenty-two biological samples (mid-stream urine and feces) were bacteriologically analyzed. The isolates were screened for ESBL production using Clinical and Laboratory Standards Institute (CLSI) breakpoints. The suspected ESBL producers were confirmed using double disc synergy test method. Out of the 162 isolates screened, 32 (19.8%) were confirmed as ESBL positive, with a prevalence of 25.32% among sick children in Enugu State University Teaching Hospital (ESUTH), Parklane, Enugu and 13.89% in apparently healthy children in a community setting. <i>Klebsiella</i> spp. and <i>Escherichia coli</i> had the highest prevalence of 34.6% and 28.6%, respectively; <i>Citrobacter</i> spp. and <i>Enterobacter</i> spp. were 18.2% and 16.7%, respectively. The ESBL positive isolates were resistant to sulfamethoxazole/trimethoprim (100%), tetracycline (100%), kanamycin (96.9%), nitrofurantoin (84.4%), ciprofloxacin (68.6%), and chloramphenicol (62.5%) but susceptible to meropenem (100%), colistin (56.3%), and gentamicin (50%). <i>Klebsiella</i> spp. had the highest ESBL occurrence among sick children while <i>E. coli</i> had the highest ESBL occurrence among healthy children in Enugu. All ESBL-positive isolates were multiply resistant to conventional antibiotics. The emergence and spread of β-lactamase-producing Enterobacteriaceae in hospital and community environments highlight the possibility for an infection outbreak if not checked. |
| first_indexed | 2024-12-14T00:20:47Z |
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| id | doaj.art-e19483950c1446e2adff492ebecbaf53 |
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| issn | 2076-3271 |
| language | English |
| last_indexed | 2024-12-14T00:20:47Z |
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| spelling | doaj.art-e19483950c1446e2adff492ebecbaf532022-12-21T23:25:15ZengMDPI AGMedical Sciences2076-32712019-11-0171110410.3390/medsci7110104medsci7110104Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, NigeriaAngus N. Oli0Vitalis I. Ogbuagu1Chika P. Ejikeugwu2Ifeanyichukwu R. Iroha3Malachy C. Ugwu4Chijioke M. Ofomata5Kenneth N. Okeke6George O. Emechebe7Jude C. Okoro8Chukwudi O. Okani9Stanley K. Onah10Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, NigeriaDepartment of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B 053, Ebonyi State, NigeriaDepartment of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B 053, Ebonyi State, NigeriaDepartment of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, NigeriaDepartment of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, Agulu, Nnamdi Azikiwe University, Awka, P.M.B 5025, Anambra State, NigeriaDepartment of Paediatrics, College of Health Sciences, Faculty of Medicine, Nnamdi Azikiwe University, Nnewi Campus, Nnewi 435101, Anambra State, Nigeria: <email>kenwados01@yahoo.co.uk</email> (K.N.O.)Department of Pediatrics, Faculty of Clinical Medicine, Chukwuemeka Odumegwu Ojukwu University, Awka Campus, Awka 420108, Anambra State, NigeriaDepartment of Paediatrics, Imo State University Teaching Hospital Orlu 473271, Imo State, NigeriaDepartment of Histopathology, Faculty of Clinical Medicine, Chukwuemeka Odumegwu Ojukwu University Teaching Hospital Amaku, Awka, 420108, Anambra State, NigeriaDepartment of Paediatrics, College of Health Sciences, Faculty of Medicine, Nnamdi Azikiwe University, Nnewi Campus, Nnewi 435101, Anambra State, Nigeria: <email>kenwados01@yahoo.co.uk</email> (K.N.O.)Extended-spectrum β-lactamase (ESBL)-producing organisms have become a serious challenge in healthcare delivery globally. The prevalence of ESBL carriage in healthy and sick children in Enugu, Nigeria, was bacteriologically investigated in this study. Four hundred and twenty-two biological samples (mid-stream urine and feces) were bacteriologically analyzed. The isolates were screened for ESBL production using Clinical and Laboratory Standards Institute (CLSI) breakpoints. The suspected ESBL producers were confirmed using double disc synergy test method. Out of the 162 isolates screened, 32 (19.8%) were confirmed as ESBL positive, with a prevalence of 25.32% among sick children in Enugu State University Teaching Hospital (ESUTH), Parklane, Enugu and 13.89% in apparently healthy children in a community setting. <i>Klebsiella</i> spp. and <i>Escherichia coli</i> had the highest prevalence of 34.6% and 28.6%, respectively; <i>Citrobacter</i> spp. and <i>Enterobacter</i> spp. were 18.2% and 16.7%, respectively. The ESBL positive isolates were resistant to sulfamethoxazole/trimethoprim (100%), tetracycline (100%), kanamycin (96.9%), nitrofurantoin (84.4%), ciprofloxacin (68.6%), and chloramphenicol (62.5%) but susceptible to meropenem (100%), colistin (56.3%), and gentamicin (50%). <i>Klebsiella</i> spp. had the highest ESBL occurrence among sick children while <i>E. coli</i> had the highest ESBL occurrence among healthy children in Enugu. All ESBL-positive isolates were multiply resistant to conventional antibiotics. The emergence and spread of β-lactamase-producing Enterobacteriaceae in hospital and community environments highlight the possibility for an infection outbreak if not checked.https://www.mdpi.com/2076-3271/7/11/104esblenterobacteriaceaegram-negative bacteriaantibiotic resistancechildhood infections |
| spellingShingle | Angus N. Oli Vitalis I. Ogbuagu Chika P. Ejikeugwu Ifeanyichukwu R. Iroha Malachy C. Ugwu Chijioke M. Ofomata Kenneth N. Okeke George O. Emechebe Jude C. Okoro Chukwudi O. Okani Stanley K. Onah Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria Medical Sciences esbl enterobacteriaceae gram-negative bacteria antibiotic resistance childhood infections |
| title | Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria |
| title_full | Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria |
| title_fullStr | Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria |
| title_full_unstemmed | Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria |
| title_short | Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria |
| title_sort | multi antibiotic resistance and factors affecting carriage of extended spectrum β lactamase producing enterobacteriaceae in pediatric population of enugu metropolis nigeria |
| topic | esbl enterobacteriaceae gram-negative bacteria antibiotic resistance childhood infections |
| url | https://www.mdpi.com/2076-3271/7/11/104 |
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