Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine
A molecularly imprinted polymer-based pencil graphite electrode (MIP PGE) sensor, modified with gold nanoparticles, was utilized for the detection of dopamine in the presence of other biochemical compounds using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), depending on its stron...
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MDPI AG
2022-02-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/3/87 |
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| author | Nemah Abu Shama Süleyman Aşır Mehmet Ozsoz Ilgım Göktürk Deniz Türkmen Fatma Yılmaz Adil Denizli |
| author_facet | Nemah Abu Shama Süleyman Aşır Mehmet Ozsoz Ilgım Göktürk Deniz Türkmen Fatma Yılmaz Adil Denizli |
| author_sort | Nemah Abu Shama |
| collection | DOAJ |
| description | A molecularly imprinted polymer-based pencil graphite electrode (MIP PGE) sensor, modified with gold nanoparticles, was utilized for the detection of dopamine in the presence of other biochemical compounds using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), depending on its strong electroactivity function. The pulse voltammetry methods recorded the highest response. In addition to the high oxidation rate of DA and the other biomolecule interferences available in the sample matrix used, which cause overlapping voltammograms, we aimed to differentiate them in a highly sensitive limit of detection range. The calibration curves for DA were obtained using the CV and DPV over the concentration range of 0.395–3.96 nM in 0.1 M phosphate buffer solution (PBS) at pH 7.4 with a correlation coefficient of 0.996 and a detection limit of 0.193 nM. The electrochemical technique was employed to detect DA molecules quantitatively in human blood plasma selected as real samples without applying any pre-treatment processes. MIP electrodes proved their ability to detect DA with high selectivity, even with epinephrine and norepinephrine competitor molecules and interferences, such as ascorbic acid (AA). The high level of recognition achieved by molecularly imprinted polymers (MIPs) is essential for many biological and pharmaceutical studies. |
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| id | doaj.art-e19555597a574cfd99554358456838d3 |
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| language | English |
| last_indexed | 2024-03-09T20:07:13Z |
| publishDate | 2022-02-01 |
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| series | Bioengineering |
| spelling | doaj.art-e19555597a574cfd99554358456838d32023-11-24T00:30:02ZengMDPI AGBioengineering2306-53542022-02-01938710.3390/bioengineering9030087Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of DopamineNemah Abu Shama0Süleyman Aşır1Mehmet Ozsoz2Ilgım Göktürk3Deniz Türkmen4Fatma Yılmaz5Adil Denizli6Department of Analytical Chemistry, Faculty of Pharmacy, Near East University, Nicosia 99138, CyprusDepartment of Materials Science and Nanotechnology Engineering, Near East University, Nicosia 99138, CyprusDepartment of Biomedical Engineering, Faculty of Engineering, Near East University, Nicosia 99138, CyprusDepartment of Chemistry, Faculty of Science, Hacettepe University, Beytepe, Ankara 06800, TurkeyDepartment of Chemistry, Faculty of Science, Hacettepe University, Beytepe, Ankara 06800, TurkeyChemistry Technology Division, Vocational School of Gerede, Bolu Abant Izzet Baysal University, Bolu 14900, TurkeyDepartment of Chemistry, Faculty of Science, Hacettepe University, Beytepe, Ankara 06800, TurkeyA molecularly imprinted polymer-based pencil graphite electrode (MIP PGE) sensor, modified with gold nanoparticles, was utilized for the detection of dopamine in the presence of other biochemical compounds using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), depending on its strong electroactivity function. The pulse voltammetry methods recorded the highest response. In addition to the high oxidation rate of DA and the other biomolecule interferences available in the sample matrix used, which cause overlapping voltammograms, we aimed to differentiate them in a highly sensitive limit of detection range. The calibration curves for DA were obtained using the CV and DPV over the concentration range of 0.395–3.96 nM in 0.1 M phosphate buffer solution (PBS) at pH 7.4 with a correlation coefficient of 0.996 and a detection limit of 0.193 nM. The electrochemical technique was employed to detect DA molecules quantitatively in human blood plasma selected as real samples without applying any pre-treatment processes. MIP electrodes proved their ability to detect DA with high selectivity, even with epinephrine and norepinephrine competitor molecules and interferences, such as ascorbic acid (AA). The high level of recognition achieved by molecularly imprinted polymers (MIPs) is essential for many biological and pharmaceutical studies.https://www.mdpi.com/2306-5354/9/3/87dopaminegold nanoparticlespencil graphite electrodemolecularly imprinted polymerdifferential pulse voltammetrycyclic voltammetry |
| spellingShingle | Nemah Abu Shama Süleyman Aşır Mehmet Ozsoz Ilgım Göktürk Deniz Türkmen Fatma Yılmaz Adil Denizli Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine Bioengineering dopamine gold nanoparticles pencil graphite electrode molecularly imprinted polymer differential pulse voltammetry cyclic voltammetry |
| title | Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine |
| title_full | Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine |
| title_fullStr | Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine |
| title_full_unstemmed | Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine |
| title_short | Gold-Modified Molecularly Imprinted <i>N</i>-Methacryloyl-(<i><span style="font-variant: small-caps">l</span></i>)-phenylalanine-containing Electrodes for Electrochemical Detection of Dopamine |
| title_sort | gold modified molecularly imprinted i n i methacryloyl i span style font variant small caps l span i phenylalanine containing electrodes for electrochemical detection of dopamine |
| topic | dopamine gold nanoparticles pencil graphite electrode molecularly imprinted polymer differential pulse voltammetry cyclic voltammetry |
| url | https://www.mdpi.com/2306-5354/9/3/87 |
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