Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens
IntroductionCecropin AD (CAD), a renowned antimicrobial peptide, has shown promising potential in treating various bacterial infections. This study investigates the protective effects of CAD against lipopolysaccharide (LPS)-induced intestinal adversities in chickens.MethodsSixty SPF-grade chicks wer...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1290182/full |
| _version_ | 1797391251332399104 |
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| author | Yan Zhi Tingyu Li Yaxuan Li Tao Zhang Mengze Du Qian Zhang Xiangdong Wang Ge Hu |
| author_facet | Yan Zhi Tingyu Li Yaxuan Li Tao Zhang Mengze Du Qian Zhang Xiangdong Wang Ge Hu |
| author_sort | Yan Zhi |
| collection | DOAJ |
| description | IntroductionCecropin AD (CAD), a renowned antimicrobial peptide, has shown promising potential in treating various bacterial infections. This study investigates the protective effects of CAD against lipopolysaccharide (LPS)-induced intestinal adversities in chickens.MethodsSixty SPF-grade chicks were divided into groups and exposed to different dosages of CAD, followed by LPS administration. The study assessed the impact of CAD on intestinal mucosal injury markers, oxidative stress, and inflammation.ResultsLPS significantly increased Diamine oxidase (DAO) and D-lactate (D-LA) levels, both indicators of intestinal mucosal injury. CAD treatment substantially attenuated these elevations, particularly at higher dosages. Additionally, CAD markedly reduced oxidative stress in intestinal tissues, as shown by normalized antioxidant levels and decreased reactive oxygen species. Histological analysis supported these findings, showing better-preserved villi structures in CAD-treated groups. Furthermore, CAD significantly reduced IL-6 and IL-8 expression post-LPS stimulation and effectively regulated the NLRP3 inflammasome pathway, decreasing associated factors like NLRP3, Caspase-1, IL-1b, and IL-18.DiscussionThe study demonstrates CAD's therapeutic potential in alleviating LPS-induced intestinal injuries. The protective effects are primarily attributed to its anti-inflammatory and antioxidative actions and modulation of the NLRP3 inflammasome pathway. |
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| format | Article |
| id | doaj.art-e1986c32cb9049f8b6d394b0d51bfabd |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-08T23:30:13Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e1986c32cb9049f8b6d394b0d51bfabd2023-12-14T15:17:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-12-011410.3389/fimmu.2023.12901821290182Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickensYan Zhi0Tingyu Li1Yaxuan Li2Tao Zhang3Mengze Du4Qian Zhang5Xiangdong Wang6Ge Hu7College of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaCollege of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaCollege of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaCollege of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaCollege of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaCollege of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaDepartment of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, the Key Laboratory of Otolaryngology-Head and Neck Surgery (Ministry of Education of China), Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, ChinaCollege of Animal Science and Technology, Beijing University of Agriculture, Beijing, ChinaIntroductionCecropin AD (CAD), a renowned antimicrobial peptide, has shown promising potential in treating various bacterial infections. This study investigates the protective effects of CAD against lipopolysaccharide (LPS)-induced intestinal adversities in chickens.MethodsSixty SPF-grade chicks were divided into groups and exposed to different dosages of CAD, followed by LPS administration. The study assessed the impact of CAD on intestinal mucosal injury markers, oxidative stress, and inflammation.ResultsLPS significantly increased Diamine oxidase (DAO) and D-lactate (D-LA) levels, both indicators of intestinal mucosal injury. CAD treatment substantially attenuated these elevations, particularly at higher dosages. Additionally, CAD markedly reduced oxidative stress in intestinal tissues, as shown by normalized antioxidant levels and decreased reactive oxygen species. Histological analysis supported these findings, showing better-preserved villi structures in CAD-treated groups. Furthermore, CAD significantly reduced IL-6 and IL-8 expression post-LPS stimulation and effectively regulated the NLRP3 inflammasome pathway, decreasing associated factors like NLRP3, Caspase-1, IL-1b, and IL-18.DiscussionThe study demonstrates CAD's therapeutic potential in alleviating LPS-induced intestinal injuries. The protective effects are primarily attributed to its anti-inflammatory and antioxidative actions and modulation of the NLRP3 inflammasome pathway.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1290182/fullCecropin AD (CAD)lipopolysaccharide (LPS)intestinal mucosal injuryoxidative stressinflammatory markersNLRP3 inflammasome |
| spellingShingle | Yan Zhi Tingyu Li Yaxuan Li Tao Zhang Mengze Du Qian Zhang Xiangdong Wang Ge Hu Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens Frontiers in Immunology Cecropin AD (CAD) lipopolysaccharide (LPS) intestinal mucosal injury oxidative stress inflammatory markers NLRP3 inflammasome |
| title | Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens |
| title_full | Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens |
| title_fullStr | Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens |
| title_full_unstemmed | Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens |
| title_short | Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens |
| title_sort | protective role of cecropin ad against lps induced intestinal mucosal injury in chickens |
| topic | Cecropin AD (CAD) lipopolysaccharide (LPS) intestinal mucosal injury oxidative stress inflammatory markers NLRP3 inflammasome |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1290182/full |
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