Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial
Abstract Background Prestorage leukoreduction of red blood cell (RBC) bags prevents accumulation of pro‐inflammatory mediators and experimentally attenuates post‐transfusion inflammation in healthy dogs. However, the effect of leukoreduction on post‐transfusion inflammation in critically ill dogs is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16487 |
| _version_ | 1811288028789866496 |
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| author | Melissa A. Claus Denise Poh Lisa Smart Sarah L. Purcell Corrin J. Boyd Claire R. Sharp |
| author_facet | Melissa A. Claus Denise Poh Lisa Smart Sarah L. Purcell Corrin J. Boyd Claire R. Sharp |
| author_sort | Melissa A. Claus |
| collection | DOAJ |
| description | Abstract Background Prestorage leukoreduction of red blood cell (RBC) bags prevents accumulation of pro‐inflammatory mediators and experimentally attenuates post‐transfusion inflammation in healthy dogs. However, the effect of leukoreduction on post‐transfusion inflammation in critically ill dogs is unclear. Hypothesis Dogs transfused with leukoreduced (LR) RBC will have lower concentrations of leukocytes, interleukin (IL)‐6, IL‐8, monocyte chemoattractant protein‐1 (MCP‐1), and C‐reactive protein (CRP) within 24 hours of post‐transfusion compared to dogs transfused with nonleukoreduced (NLR) RBC. Animals Sixty‐one RBC‐transfused dogs (LR = 34, NLR = 27). Methods Randomized, blinded, controlled preliminary clinical trial. Blood bag processing was randomized to create identically appearing LR and NLR bags. Group allocation occurred with transfusion of the oldest compatible RBC bag. Blood samples were collected pretransfusion and at 8 and 24 hours post‐transfusion for leukocyte count, IL‐6, IL‐8, MCP‐1, and CRP. Data were analyzed on an intention‐to‐treat basis using linear mixed effects models. Significance was set at P < .05. Results No significant differences were found between groups in concentrations of leukocytes (P = .93), IL‐6 (P = .99), IL‐8 (P = .75), MCP‐1 (P = .69), or CRP (P = .18) over time. Eleven LR dogs (32%) and 4 NLR dogs (15%) were euthanized in the hospital (P = .14). No natural deaths occurred. Conclusions and Clinical Importance No differences in inflammation biomarker concentrations were detected over time between dogs transfused with LR or NLR RBC, but heterogeneity likely hampered the ability to detect a difference with this sample size. The novel randomization and enrollment protocol was successfully implemented across 2 participating institutions and will be easily scaled up for a future multicenter clinical trial. |
| first_indexed | 2024-04-13T03:28:50Z |
| format | Article |
| id | doaj.art-e19dcb02a7b04d8881b1d8c4f24fd95b |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-13T03:28:50Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-e19dcb02a7b04d8881b1d8c4f24fd95b2022-12-22T03:04:33ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762022-07-013641248125710.1111/jvim.16487Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trialMelissa A. Claus0Denise Poh1Lisa Smart2Sarah L. Purcell3Corrin J. Boyd4Claire R. Sharp5Murdoch University, School of Veterinary Medicine Murdoch Western Australia AustraliaMurdoch University, School of Veterinary Medicine Murdoch Western Australia AustraliaMurdoch University, School of Veterinary Medicine Murdoch Western Australia AustraliaUniversity of Queensland, School of Veterinary Science Gatton Queensland AustraliaMurdoch University, School of Veterinary Medicine Murdoch Western Australia AustraliaMurdoch University, School of Veterinary Medicine Murdoch Western Australia AustraliaAbstract Background Prestorage leukoreduction of red blood cell (RBC) bags prevents accumulation of pro‐inflammatory mediators and experimentally attenuates post‐transfusion inflammation in healthy dogs. However, the effect of leukoreduction on post‐transfusion inflammation in critically ill dogs is unclear. Hypothesis Dogs transfused with leukoreduced (LR) RBC will have lower concentrations of leukocytes, interleukin (IL)‐6, IL‐8, monocyte chemoattractant protein‐1 (MCP‐1), and C‐reactive protein (CRP) within 24 hours of post‐transfusion compared to dogs transfused with nonleukoreduced (NLR) RBC. Animals Sixty‐one RBC‐transfused dogs (LR = 34, NLR = 27). Methods Randomized, blinded, controlled preliminary clinical trial. Blood bag processing was randomized to create identically appearing LR and NLR bags. Group allocation occurred with transfusion of the oldest compatible RBC bag. Blood samples were collected pretransfusion and at 8 and 24 hours post‐transfusion for leukocyte count, IL‐6, IL‐8, MCP‐1, and CRP. Data were analyzed on an intention‐to‐treat basis using linear mixed effects models. Significance was set at P < .05. Results No significant differences were found between groups in concentrations of leukocytes (P = .93), IL‐6 (P = .99), IL‐8 (P = .75), MCP‐1 (P = .69), or CRP (P = .18) over time. Eleven LR dogs (32%) and 4 NLR dogs (15%) were euthanized in the hospital (P = .14). No natural deaths occurred. Conclusions and Clinical Importance No differences in inflammation biomarker concentrations were detected over time between dogs transfused with LR or NLR RBC, but heterogeneity likely hampered the ability to detect a difference with this sample size. The novel randomization and enrollment protocol was successfully implemented across 2 participating institutions and will be easily scaled up for a future multicenter clinical trial.https://doi.org/10.1111/jvim.16487C‐reactive proteinfebrile nonhemolytic transfusion reactioninterleukin‐6interleukin‐8leukocytesmonocyte chemoattractant protein‐1 |
| spellingShingle | Melissa A. Claus Denise Poh Lisa Smart Sarah L. Purcell Corrin J. Boyd Claire R. Sharp Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial Journal of Veterinary Internal Medicine C‐reactive protein febrile nonhemolytic transfusion reaction interleukin‐6 interleukin‐8 leukocytes monocyte chemoattractant protein‐1 |
| title | Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial |
| title_full | Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial |
| title_fullStr | Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial |
| title_full_unstemmed | Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial |
| title_short | Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial |
| title_sort | effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions a randomized blinded controlled clinical trial |
| topic | C‐reactive protein febrile nonhemolytic transfusion reaction interleukin‐6 interleukin‐8 leukocytes monocyte chemoattractant protein‐1 |
| url | https://doi.org/10.1111/jvim.16487 |
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