Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort)
Epidemiologic studies have indicated that cruciferous vegetables can influence the cancer risk; therefore, we examined with a cross-sectional approach the correlation between the frequent consumption of the total cruciferous vegetables and the formation of bulky DNA damage, a biomarker of carcinogen...
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MDPI AG
2022-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/12/2477 |
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| author | Marco Peluso Armelle Munnia Valentina Russo Andrea Galli Valeria Pala Yvonne T. van der Schouw Matthias B. Schulze Elisabete Weiderpass Rosario Tumino Calogero Saieva Amiano Exezarreta Pilar Dagfinn Aune Alicia K. Heath Elom Aglago Antonio Agudo Salvatore Panico Kristina Elin Nielsen Petersen Anne Tjønneland Lluís Cirera Miguel Rodriguez-Barranco Verena Katzke Rudolf Kaaks Fulvio Ricceri Lorenzo Milani Paolo Vineis Carlotta Sacerdote |
| author_facet | Marco Peluso Armelle Munnia Valentina Russo Andrea Galli Valeria Pala Yvonne T. van der Schouw Matthias B. Schulze Elisabete Weiderpass Rosario Tumino Calogero Saieva Amiano Exezarreta Pilar Dagfinn Aune Alicia K. Heath Elom Aglago Antonio Agudo Salvatore Panico Kristina Elin Nielsen Petersen Anne Tjønneland Lluís Cirera Miguel Rodriguez-Barranco Verena Katzke Rudolf Kaaks Fulvio Ricceri Lorenzo Milani Paolo Vineis Carlotta Sacerdote |
| author_sort | Marco Peluso |
| collection | DOAJ |
| description | Epidemiologic studies have indicated that cruciferous vegetables can influence the cancer risk; therefore, we examined with a cross-sectional approach the correlation between the frequent consumption of the total cruciferous vegetables and the formation of bulky DNA damage, a biomarker of carcinogen exposure and cancer risk, in the Gen-Air study within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. DNA damage measurements were performed in the peripheral blood of 696 of those apparently healthy without cancer controls, including 379 never-smokers and 317 former smokers from seven European countries by the <sup>32</sup>P-postlabeling assay. In the Gen-Air controls, the median intake of cruciferous vegetables was 6.16 (IQR 1.16–13.66) g/day, ranging from 0.37 (IQR 0–6.00) g/day in Spain to 11.34 (IQR 6.02–16.07) g/day in the UK. Based on this information, participants were grouped into: (a) high consumers (>20 g/day), (b) medium consumers (3–20 g/day) and (c) low consumers (<3.0 g/day). Overall, low cruciferous vegetable intake was correlated with a greater frequency of bulky DNA lesions, including benzo(a)pyrene, lactone and quinone-adducts and bulky oxidative lesions, in the adjusted models. Conversely, a high versus low intake of cruciferous vegetables was associated with a reduction in DNA damage (up to a 23% change, <i>p</i> = 0.032); this was particularly evident in former smokers (up to a 40% change, <i>p</i> = 0.008). The Generalized Linear Regression models indicated an overall Mean Ratio between the high and the low consumers of 0.78 (95% confidence interval, 0.64–0.97). The current study suggests that a higher intake of cruciferous vegetables is associated with a lower level of bulky DNA adducts and supports the potential for cancer prevention strategies through dietary habit changes aimed at increasing the consumption of cruciferous vegetables. |
| first_indexed | 2024-03-09T22:50:45Z |
| format | Article |
| id | doaj.art-e19e2db8887f479081ffc29c6eb499c1 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T22:50:45Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-e19e2db8887f479081ffc29c6eb499c12023-11-23T18:21:49ZengMDPI AGNutrients2072-66432022-06-011412247710.3390/nu14122477Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort)Marco Peluso0Armelle Munnia1Valentina Russo2Andrea Galli3Valeria Pala4Yvonne T. van der Schouw5Matthias B. Schulze6Elisabete Weiderpass7Rosario Tumino8Calogero Saieva9Amiano Exezarreta Pilar10Dagfinn Aune11Alicia K. Heath12Elom Aglago13Antonio Agudo14Salvatore Panico15Kristina Elin Nielsen Petersen16Anne Tjønneland17Lluís Cirera18Miguel Rodriguez-Barranco19Verena Katzke20Rudolf Kaaks21Fulvio Ricceri22Lorenzo Milani23Paolo Vineis24Carlotta Sacerdote25Research Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyResearch Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyResearch Branch, Regional Cancer Prevention Laboratory, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, 50139 Florence, ItalyEpidemiology and Prevention Unit, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, 20133 Milan, ItalyJulius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, 3508 GA Utrecht, The NetherlandsDepartment of Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke, 14558 Nuthetal, GermanyInternational Agency for Research on Cancer, World Health Organization, 69372 Lyon, FranceHyblean Association for Epidemiological Research, AIRE ONLUS, 97100 Ragusa, ItalyCancer Risk Factors and Life-Style Epidemiology Unit, ISPRO-Study, Prevention and Oncology Network Institute, 50139 Florence, ItalyMinistry of Health of the Basque Government, Sub Directorate for Public Health and Addictions of Gipuzkoa, 20014 San Sebastian, SpainDepartment of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London W2 1PG, UKDepartment of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London W2 1PG, UKDepartment of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London W2 1PG, UKUnit of Nutrition and Cancer, Catalan Institute of Oncology-ICO, 08908 L’Hospitalet de Llobregat, SpainDepartment of Clinical Medicine and Surgery, Federico II University, 80138 Naples, ItalyDanish Cancer Society Research Center, Diet, Cancer and Health, DK-2100 Copenhagen, DenmarkDanish Cancer Society Research Center, Diet, Cancer and Health, DK-2100 Copenhagen, DenmarkDepartment of Epidemiology, Regional Health Council-IMIB–Arrixaca, 30120 Murcia, SpainCIBER de Epidemiología y Salud Pública (CIBERESP), 28028 Madrid, SpainDivision of Cancer Epidemiology, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanyDivision of Cancer Epidemiology, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanyDepartment of Clinical and Biological Sciences, University of Turin, 10124 Turin, ItalyDepartment of Clinical and Biological Sciences, University of Turin, 10124 Turin, ItalyMRC Centre for Environment and Health School of Public Health, Imperial College LondonSt Mary’s Campus, Norfolk Place, London W2 1PG, UKUnit of Cancer Epidemiology, Città Della Salute e Della Scienza University-Hospital and Center for Cancer Prevention (CPO), 10126 Turin, ItalyEpidemiologic studies have indicated that cruciferous vegetables can influence the cancer risk; therefore, we examined with a cross-sectional approach the correlation between the frequent consumption of the total cruciferous vegetables and the formation of bulky DNA damage, a biomarker of carcinogen exposure and cancer risk, in the Gen-Air study within the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. DNA damage measurements were performed in the peripheral blood of 696 of those apparently healthy without cancer controls, including 379 never-smokers and 317 former smokers from seven European countries by the <sup>32</sup>P-postlabeling assay. In the Gen-Air controls, the median intake of cruciferous vegetables was 6.16 (IQR 1.16–13.66) g/day, ranging from 0.37 (IQR 0–6.00) g/day in Spain to 11.34 (IQR 6.02–16.07) g/day in the UK. Based on this information, participants were grouped into: (a) high consumers (>20 g/day), (b) medium consumers (3–20 g/day) and (c) low consumers (<3.0 g/day). Overall, low cruciferous vegetable intake was correlated with a greater frequency of bulky DNA lesions, including benzo(a)pyrene, lactone and quinone-adducts and bulky oxidative lesions, in the adjusted models. Conversely, a high versus low intake of cruciferous vegetables was associated with a reduction in DNA damage (up to a 23% change, <i>p</i> = 0.032); this was particularly evident in former smokers (up to a 40% change, <i>p</i> = 0.008). The Generalized Linear Regression models indicated an overall Mean Ratio between the high and the low consumers of 0.78 (95% confidence interval, 0.64–0.97). The current study suggests that a higher intake of cruciferous vegetables is associated with a lower level of bulky DNA adducts and supports the potential for cancer prevention strategies through dietary habit changes aimed at increasing the consumption of cruciferous vegetables.https://www.mdpi.com/2072-6643/14/12/2477EPICdietcruciferous vegetablesDNA damageB(a)P-adductsbulky oxidative lesions |
| spellingShingle | Marco Peluso Armelle Munnia Valentina Russo Andrea Galli Valeria Pala Yvonne T. van der Schouw Matthias B. Schulze Elisabete Weiderpass Rosario Tumino Calogero Saieva Amiano Exezarreta Pilar Dagfinn Aune Alicia K. Heath Elom Aglago Antonio Agudo Salvatore Panico Kristina Elin Nielsen Petersen Anne Tjønneland Lluís Cirera Miguel Rodriguez-Barranco Verena Katzke Rudolf Kaaks Fulvio Ricceri Lorenzo Milani Paolo Vineis Carlotta Sacerdote Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort) Nutrients EPIC diet cruciferous vegetables DNA damage B(a)P-adducts bulky oxidative lesions |
| title | Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort) |
| title_full | Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort) |
| title_fullStr | Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort) |
| title_full_unstemmed | Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort) |
| title_short | Cruciferous Vegetable Intake and Bulky DNA Damage within Non-Smokers and Former Smokers in the Gen-Air Study (EPIC Cohort) |
| title_sort | cruciferous vegetable intake and bulky dna damage within non smokers and former smokers in the gen air study epic cohort |
| topic | EPIC diet cruciferous vegetables DNA damage B(a)P-adducts bulky oxidative lesions |
| url | https://www.mdpi.com/2072-6643/14/12/2477 |
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