Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants
Abstract Bacillus is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and...
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| Format: | Article |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-48171-9 |
| _version_ | 1797415434163585024 |
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| author | Yunxin Shen Zhufeng Shi Jiangyuan Zhao Minggang Li Jiacai Tang Nan Wang Yanfang Mo Tongyu Yang Xudong Zhou Qibin Chen Peiweng Yang |
| author_facet | Yunxin Shen Zhufeng Shi Jiangyuan Zhao Minggang Li Jiacai Tang Nan Wang Yanfang Mo Tongyu Yang Xudong Zhou Qibin Chen Peiweng Yang |
| author_sort | Yunxin Shen |
| collection | DOAJ |
| description | Abstract Bacillus is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and can be colonized in the rhizosphere of a variety of plants. This study will further explore its mechanism by combining biological and molecular biology methods. SH-1471 contains a ring chromosome of 4,181,346 bp with a mean G + C content of 46.18%. We identified 14 homologous genes related to biosynthesis of resistant secondary metabolite, and three clusters encoded potential new antibacterial substances. It also contains a large number of genes from colonizing bacteria and genes related to plant bacterial interactions. It also contains genes related to environmental stress, as well as genes related to drug resistance. We also found that there are many metabolites in the strain that can inhibit the growth of pathogens. In addition, our indoor pot test found that SH-1471 has a good control effect on tomato wilt, and could significantly improve plant height, stem circumference, root length, root weight, and fresh weight and dry weight of the aboveground part of tomato seedlings. Therefore, SH-1471 is a potential biological control strain with important application value. The results of this study will help to further study the mechanism of SH-1471 in biological control of plant diseases and promote its application. |
| first_indexed | 2024-03-09T05:48:36Z |
| format | Article |
| id | doaj.art-e1a038142c0e41f187b92fc1d7d4a31e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T05:48:36Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e1a038142c0e41f187b92fc1d7d4a31e2023-12-03T12:19:32ZengNature PortfolioScientific Reports2045-23222023-11-0113111910.1038/s41598-023-48171-9Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plantsYunxin Shen0Zhufeng Shi1Jiangyuan Zhao2Minggang Li3Jiacai Tang4Nan Wang5Yanfang Mo6Tongyu Yang7Xudong Zhou8Qibin Chen9Peiweng Yang10Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesYunnan Institute of Microbiology, Yunnan UniversityYunnan Institute of Microbiology, Yunnan UniversityInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesCollege of Plant Protection, Yunnan Agricultural UniversityInstitute of Agricultural Environment and Resources, Yunnan Academy of Agricultural SciencesAbstract Bacillus is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and can be colonized in the rhizosphere of a variety of plants. This study will further explore its mechanism by combining biological and molecular biology methods. SH-1471 contains a ring chromosome of 4,181,346 bp with a mean G + C content of 46.18%. We identified 14 homologous genes related to biosynthesis of resistant secondary metabolite, and three clusters encoded potential new antibacterial substances. It also contains a large number of genes from colonizing bacteria and genes related to plant bacterial interactions. It also contains genes related to environmental stress, as well as genes related to drug resistance. We also found that there are many metabolites in the strain that can inhibit the growth of pathogens. In addition, our indoor pot test found that SH-1471 has a good control effect on tomato wilt, and could significantly improve plant height, stem circumference, root length, root weight, and fresh weight and dry weight of the aboveground part of tomato seedlings. Therefore, SH-1471 is a potential biological control strain with important application value. The results of this study will help to further study the mechanism of SH-1471 in biological control of plant diseases and promote its application.https://doi.org/10.1038/s41598-023-48171-9 |
| spellingShingle | Yunxin Shen Zhufeng Shi Jiangyuan Zhao Minggang Li Jiacai Tang Nan Wang Yanfang Mo Tongyu Yang Xudong Zhou Qibin Chen Peiweng Yang Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants Scientific Reports |
| title | Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants |
| title_full | Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants |
| title_fullStr | Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants |
| title_full_unstemmed | Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants |
| title_short | Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants |
| title_sort | whole genome sequencing provides evidence for bacillus velezensis sh 1471 as a beneficial rhizosphere bacterium in plants |
| url | https://doi.org/10.1038/s41598-023-48171-9 |
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