Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging
Abstract Background Hypercholesterolemia is known to increase the risk of AD in later life, the purpose of this study is to illustrate brain metabolic and structural changes in a cholesterol-fed rabbit model of Alzheimer’s Disease (AD) by using clinical 3 T Magnetic Resonance Imaging (MRI). Methods...
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-018-0705-9 |
| _version_ | 1819101493165293568 |
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| author | Ping Jin Yongming Pan Zhiyong Pan Jianqin Xu Min Lin Zhichao Sun Minli Chen Maosheng Xu |
| author_facet | Ping Jin Yongming Pan Zhiyong Pan Jianqin Xu Min Lin Zhichao Sun Minli Chen Maosheng Xu |
| author_sort | Ping Jin |
| collection | DOAJ |
| description | Abstract Background Hypercholesterolemia is known to increase the risk of AD in later life, the purpose of this study is to illustrate brain metabolic and structural changes in a cholesterol-fed rabbit model of Alzheimer’s Disease (AD) by using clinical 3 T Magnetic Resonance Imaging (MRI). Methods The Institutional Animal Care and Use Committee of Zhejiang Chinese Medical University approved the study. Totally 16 Japanese White Rabbits (JWR) were randomly divided into 2 groups including normal control group fed with routine diet (group NC) and high cholesterol diet group (group CD) fed a 2% cholesterol diet with 0.24 ppm copper in the drinking water for 12 weeks. Magnetic resonance spectroscopy (MRS) and structural image of rabbit brain were performed by using a 3 Tesla (T) MRI scanner with an 8 channel Rabbit coil. The chemical metabolites were identified by LC Model including N-acetylaspartate (NAA), creatine (Cr), glutamate (Glu), glutamine (Gln), Glycerophosphatidylcholine (GPC), phosphorylcholine (PCH), and myoinositol (MI). The relative concentrations (/Cr) were analyzed. Additionally, Amyloid-β (Aβ) accumulation in the brain was measured postmortem. For comparisons of MR and Aβ data between groups, two-tailed t-tests were performed. Results The ratio of NAA/Cr (0.76 ± 0.10) and Glu/Cr (0.90 ± 0.14) in group CD were lower than those in the group NC (0.87 ± 0.06, 1.13 ± 0.22, respectively, P < 0.05). Compared to the group NC (2.88 ± 0.09 cm3, 0.63 ± 0.08 cm3, respectively), the cortical and hippocampal volumes (2.60 ± 0.14 cm3 and 0.47 ± 0.07 cm3, respectively) of rabbits brain decreased in the group CD while the third and lateral ventricular volumes enlarged (44.56 ± 6.01 mm3 vs 31.40 ± 6.14 mm3, 261.40 ± 30.98 mm3 vs 153.81 ± 30.08 mm3, P < 0.05). These metabolic and structural changes were additionally accompanied by the significant increase of Aβ1–42 in the cortex and hippocampus (163.60 ± 16.26 pg/mg and 215.20 ± 69.86 pg/mg, respectively, P < 0.05). Conclusion High cholesterol diet can induce the brain metabolic and structural changes of the rabbit including lowered level of NAA and Glu and the atrophy of the brain which were similar to those of human AD. |
| first_indexed | 2024-12-22T01:19:32Z |
| format | Article |
| id | doaj.art-e1a2faefbf5e44efb9a9059cb25c606e |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-22T01:19:32Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
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| series | Lipids in Health and Disease |
| spelling | doaj.art-e1a2faefbf5e44efb9a9059cb25c606e2022-12-21T18:43:47ZengBMCLipids in Health and Disease1476-511X2018-03-011711810.1186/s12944-018-0705-9Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imagingPing Jin0Yongming Pan1Zhiyong Pan2Jianqin Xu3Min Lin4Zhichao Sun5Minli Chen6Maosheng Xu7The First Affiliated Hospital of Zhejiang Chinese Medical UniversityLaboratory Animal Research Center/Comparative Medical Research Institute, Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical UniversityLaboratory Animal Research Center/Comparative Medical Research Institute, Zhejiang Chinese Medical UniversityThe Third Affiliated Hospital of Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical UniversityLaboratory Animal Research Center/Comparative Medical Research Institute, Zhejiang Chinese Medical UniversityThe First Affiliated Hospital of Zhejiang Chinese Medical UniversityAbstract Background Hypercholesterolemia is known to increase the risk of AD in later life, the purpose of this study is to illustrate brain metabolic and structural changes in a cholesterol-fed rabbit model of Alzheimer’s Disease (AD) by using clinical 3 T Magnetic Resonance Imaging (MRI). Methods The Institutional Animal Care and Use Committee of Zhejiang Chinese Medical University approved the study. Totally 16 Japanese White Rabbits (JWR) were randomly divided into 2 groups including normal control group fed with routine diet (group NC) and high cholesterol diet group (group CD) fed a 2% cholesterol diet with 0.24 ppm copper in the drinking water for 12 weeks. Magnetic resonance spectroscopy (MRS) and structural image of rabbit brain were performed by using a 3 Tesla (T) MRI scanner with an 8 channel Rabbit coil. The chemical metabolites were identified by LC Model including N-acetylaspartate (NAA), creatine (Cr), glutamate (Glu), glutamine (Gln), Glycerophosphatidylcholine (GPC), phosphorylcholine (PCH), and myoinositol (MI). The relative concentrations (/Cr) were analyzed. Additionally, Amyloid-β (Aβ) accumulation in the brain was measured postmortem. For comparisons of MR and Aβ data between groups, two-tailed t-tests were performed. Results The ratio of NAA/Cr (0.76 ± 0.10) and Glu/Cr (0.90 ± 0.14) in group CD were lower than those in the group NC (0.87 ± 0.06, 1.13 ± 0.22, respectively, P < 0.05). Compared to the group NC (2.88 ± 0.09 cm3, 0.63 ± 0.08 cm3, respectively), the cortical and hippocampal volumes (2.60 ± 0.14 cm3 and 0.47 ± 0.07 cm3, respectively) of rabbits brain decreased in the group CD while the third and lateral ventricular volumes enlarged (44.56 ± 6.01 mm3 vs 31.40 ± 6.14 mm3, 261.40 ± 30.98 mm3 vs 153.81 ± 30.08 mm3, P < 0.05). These metabolic and structural changes were additionally accompanied by the significant increase of Aβ1–42 in the cortex and hippocampus (163.60 ± 16.26 pg/mg and 215.20 ± 69.86 pg/mg, respectively, P < 0.05). Conclusion High cholesterol diet can induce the brain metabolic and structural changes of the rabbit including lowered level of NAA and Glu and the atrophy of the brain which were similar to those of human AD.http://link.springer.com/article/10.1186/s12944-018-0705-9CholesterolAmyloid βRabbitMagnetic resonance imagingMagnetic resonance spectroscopyBrain metabolite |
| spellingShingle | Ping Jin Yongming Pan Zhiyong Pan Jianqin Xu Min Lin Zhichao Sun Minli Chen Maosheng Xu Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging Lipids in Health and Disease Cholesterol Amyloid β Rabbit Magnetic resonance imaging Magnetic resonance spectroscopy Brain metabolite |
| title | Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging |
| title_full | Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging |
| title_fullStr | Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging |
| title_full_unstemmed | Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging |
| title_short | Alzheimer-like brain metabolic and structural features in cholesterol-fed rabbit detected by magnetic resonance imaging |
| title_sort | alzheimer like brain metabolic and structural features in cholesterol fed rabbit detected by magnetic resonance imaging |
| topic | Cholesterol Amyloid β Rabbit Magnetic resonance imaging Magnetic resonance spectroscopy Brain metabolite |
| url | http://link.springer.com/article/10.1186/s12944-018-0705-9 |
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