Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique
Substrate solubility in steroid biotransformation is critical for enhancing the biotransformation of hydrophobic compounds. In this study, the sulfobutyl ether-β-cyclodextrin (SBE-β-CD) complexation technique was used for the biotransformation of β-sitosterol to androsta-1, 4-diene-3, 17-dione with...
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EDP Sciences
2016-01-01
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| Series: | MATEC Web of Conferences |
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| Online Access: | http://dx.doi.org/10.1051/matecconf/20166701001 |
| _version_ | 1818620777644163072 |
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| author | Wang Jingwen Guan Shimin Cai Baoguo Zhang Shuo Yang Shulin Zhou Yue Rong Shaofeng Li Qianqian |
| author_facet | Wang Jingwen Guan Shimin Cai Baoguo Zhang Shuo Yang Shulin Zhou Yue Rong Shaofeng Li Qianqian |
| author_sort | Wang Jingwen |
| collection | DOAJ |
| description | Substrate solubility in steroid biotransformation is critical for enhancing the biotransformation of hydrophobic compounds. In this study, the sulfobutyl ether-β-cyclodextrin (SBE-β-CD) complexation technique was used for the biotransformation of β-sitosterol to androsta-1, 4-diene-3, 17-dione with Mycobacterium ATCC25795. The production yield was increased by 26.72%, and the biotransformation course was shortened by 24h using β-sitosterol/SBE-β-CD inclusion complexes as substrates (1.0 g/L). Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that an inclusion complex was formed between SBE-β-CD and β-sitosterol. The complex significantly increased the solubility of β-sitosterol and improved the biotransformation efficiency of the substrate. |
| first_indexed | 2024-12-16T17:58:46Z |
| format | Article |
| id | doaj.art-e1a7f29d3d6c46e8afc1407df34cb873 |
| institution | Directory Open Access Journal |
| issn | 2261-236X |
| language | English |
| last_indexed | 2024-12-16T17:58:46Z |
| publishDate | 2016-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | MATEC Web of Conferences |
| spelling | doaj.art-e1a7f29d3d6c46e8afc1407df34cb8732022-12-21T22:22:07ZengEDP SciencesMATEC Web of Conferences2261-236X2016-01-01670100110.1051/matecconf/20166701001matecconf_smae2016_01001Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation TechniqueWang Jingwen0Guan Shimin1Cai Baoguo2Zhang Shuo3Yang Shulin4Zhou Yue5Rong Shaofeng6Li Qianqian7School of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySchool of Perfume and Aroma Technology, Shanghai Institute of TechnologySubstrate solubility in steroid biotransformation is critical for enhancing the biotransformation of hydrophobic compounds. In this study, the sulfobutyl ether-β-cyclodextrin (SBE-β-CD) complexation technique was used for the biotransformation of β-sitosterol to androsta-1, 4-diene-3, 17-dione with Mycobacterium ATCC25795. The production yield was increased by 26.72%, and the biotransformation course was shortened by 24h using β-sitosterol/SBE-β-CD inclusion complexes as substrates (1.0 g/L). Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that an inclusion complex was formed between SBE-β-CD and β-sitosterol. The complex significantly increased the solubility of β-sitosterol and improved the biotransformation efficiency of the substrate.http://dx.doi.org/10.1051/matecconf/20166701001β-sitosterolbiotransformationMycobacteriumandrosta-14-dien-317-dione |
| spellingShingle | Wang Jingwen Guan Shimin Cai Baoguo Zhang Shuo Yang Shulin Zhou Yue Rong Shaofeng Li Qianqian Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique MATEC Web of Conferences β-sitosterol biotransformation Mycobacterium androsta-1 4-dien-3 17-dione |
| title | Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique |
| title_full | Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique |
| title_fullStr | Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique |
| title_full_unstemmed | Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique |
| title_short | Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique |
| title_sort | increased yield of biotransformation of androsta 1 4 dien 3 17 dione from β sitosterol by using sulfobutyl ether β cyclodextrin complexation technique |
| topic | β-sitosterol biotransformation Mycobacterium androsta-1 4-dien-3 17-dione |
| url | http://dx.doi.org/10.1051/matecconf/20166701001 |
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