Complete Prevention of Bubbles in a PDMS-Based Digital PCR Chip with a Multifunction Cavity

Complete Prevention of Bubbles in a PDMS-Based Digital PCR Chip with a Multifunction Cavity

In a chamber-based digital PCR (dPCR) chip fabricated with polydimethylsiloxane (PDMS), bubble generation in the chambers at high temperatures is a critical issue. Here, we found that the main reason for bubble formation in PDMS chips is the too-high saturated vapor pressure of water at an elevated...

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Bibliographic Details
Main Authors: Shiyuan Gao, Tiegang Xu, Lei Wu, Xiaoyue Zhu, Xuefeng Wang, Ying Chen, Gang Li, Xinxin Li
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Biosensors
Subjects:
digital PCR chip
polydimethylsiloxane
multifunction cavity
water loss
bubble generation
parylene C shell
Online Access:https://www.mdpi.com/2079-6374/14/3/114

Internet

https://www.mdpi.com/2079-6374/14/3/114

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