Complete Prevention of Bubbles in a PDMS-Based Digital PCR Chip with a Multifunction Cavity
In a chamber-based digital PCR (dPCR) chip fabricated with polydimethylsiloxane (PDMS), bubble generation in the chambers at high temperatures is a critical issue. Here, we found that the main reason for bubble formation in PDMS chips is the too-high saturated vapor pressure of water at an elevated...
Main Authors: | Shiyuan Gao, Tiegang Xu, Lei Wu, Xiaoyue Zhu, Xuefeng Wang, Ying Chen, Gang Li, Xinxin Li |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2024-02-01
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Series: | Biosensors |
Subjects: | |
Online Access: | https://www.mdpi.com/2079-6374/14/3/114 |
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