Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses
Until a few years ago, many studies focused on the transcriptomic response to single stresses. However, tomato cultivations are often constrained by a wide range of biotic and abiotic stress that can occur singularly or in combination, and several genes can be involved in the defensive mechanism res...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/4061 |
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| author | Ciro Gianmaria Amoroso Daniela D’Esposito Riccardo Aiese Cigliano Maria Raffaella Ercolano |
| author_facet | Ciro Gianmaria Amoroso Daniela D’Esposito Riccardo Aiese Cigliano Maria Raffaella Ercolano |
| author_sort | Ciro Gianmaria Amoroso |
| collection | DOAJ |
| description | Until a few years ago, many studies focused on the transcriptomic response to single stresses. However, tomato cultivations are often constrained by a wide range of biotic and abiotic stress that can occur singularly or in combination, and several genes can be involved in the defensive mechanism response. Therefore, we analyzed and compared the transcriptomic responses of resistant and susceptible genotypes to seven biotic stresses (<i>Cladosporium fulvum</i>, <i>Phytophthora infestans</i>, <i>Pseudomonas syringae</i>, <i>Ralstonia solanacearum</i>, <i>Sclerotinia sclerotiorum</i>, Tomato spotted wilt virus (TSWV) and <i>Tuta absoluta</i>) and five abiotic stresses (drought, salinity, low temperatures, and oxidative stress) to identify genes involved in response to multiple stressors. With this approach, we found genes encoding for TFs, phytohormones, or participating in signaling and cell wall metabolic processes, participating in defense against various biotic and abiotic stress. Moreover, a total of 1474 DEGs were commonly found between biotic and abiotic stress. Among these, 67 DEGs were involved in response to at least four different stresses. In particular, we found RLKs, MAPKs, Fasciclin-like arabinogalactans (FLAs), glycosyltransferases, genes involved in the auxin, ET, and JA pathways, MYBs, bZIPs, WRKYs and ERFs genes. Detected genes responsive to multiple stress might be further investigated with biotechnological approaches to effectively improve plant tolerance in the field. |
| first_indexed | 2024-03-11T08:41:24Z |
| format | Article |
| id | doaj.art-e1b10acef1f545889e535a873a1b4791 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:41:24Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e1b10acef1f545889e535a873a1b47912023-11-16T21:08:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244406110.3390/ijms24044061Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress ResponsesCiro Gianmaria Amoroso0Daniela D’Esposito1Riccardo Aiese Cigliano2Maria Raffaella Ercolano3Department of Agricultural Science, University of Naples “Federico II,” 80055 Portici, ItalyDepartment of Agricultural Science, University of Naples “Federico II,” 80055 Portici, ItalySequentia Biotech SL, Calle Comte D’Urgell 240, 08036 Barcelona, SpainDepartment of Agricultural Science, University of Naples “Federico II,” 80055 Portici, ItalyUntil a few years ago, many studies focused on the transcriptomic response to single stresses. However, tomato cultivations are often constrained by a wide range of biotic and abiotic stress that can occur singularly or in combination, and several genes can be involved in the defensive mechanism response. Therefore, we analyzed and compared the transcriptomic responses of resistant and susceptible genotypes to seven biotic stresses (<i>Cladosporium fulvum</i>, <i>Phytophthora infestans</i>, <i>Pseudomonas syringae</i>, <i>Ralstonia solanacearum</i>, <i>Sclerotinia sclerotiorum</i>, Tomato spotted wilt virus (TSWV) and <i>Tuta absoluta</i>) and five abiotic stresses (drought, salinity, low temperatures, and oxidative stress) to identify genes involved in response to multiple stressors. With this approach, we found genes encoding for TFs, phytohormones, or participating in signaling and cell wall metabolic processes, participating in defense against various biotic and abiotic stress. Moreover, a total of 1474 DEGs were commonly found between biotic and abiotic stress. Among these, 67 DEGs were involved in response to at least four different stresses. In particular, we found RLKs, MAPKs, Fasciclin-like arabinogalactans (FLAs), glycosyltransferases, genes involved in the auxin, ET, and JA pathways, MYBs, bZIPs, WRKYs and ERFs genes. Detected genes responsive to multiple stress might be further investigated with biotechnological approaches to effectively improve plant tolerance in the field.https://www.mdpi.com/1422-0067/24/4/4061plant stresstranscriptomicstranscription factorssignalingcell wallphytohormones |
| spellingShingle | Ciro Gianmaria Amoroso Daniela D’Esposito Riccardo Aiese Cigliano Maria Raffaella Ercolano Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses International Journal of Molecular Sciences plant stress transcriptomics transcription factors signaling cell wall phytohormones |
| title | Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses |
| title_full | Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses |
| title_fullStr | Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses |
| title_full_unstemmed | Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses |
| title_short | Comparison of Tomato Transcriptomic Profiles Reveals Overlapping Patterns in Abiotic and Biotic Stress Responses |
| title_sort | comparison of tomato transcriptomic profiles reveals overlapping patterns in abiotic and biotic stress responses |
| topic | plant stress transcriptomics transcription factors signaling cell wall phytohormones |
| url | https://www.mdpi.com/1422-0067/24/4/4061 |
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