A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions
Diplodia seriata is a polyphagous and widespread pathogen that infects trunks, shoots, fruit and leaves of apple plants. Fungicides applied in integrated pest management programmes can act against D. seriata. However, introduction of scab-resistant apple cultivars and the consequent reduction in fun...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2019-05-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5805 |
| _version_ | 1818210571408900096 |
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| author | Elena ARRIGONI Claudia Maria OLIVEIRA LONGA Dario ANGELI Matteo SOINI Ilaria PERTOT Michele PERAZZOLLI |
| author_facet | Elena ARRIGONI Claudia Maria OLIVEIRA LONGA Dario ANGELI Matteo SOINI Ilaria PERTOT Michele PERAZZOLLI |
| author_sort | Elena ARRIGONI |
| collection | DOAJ |
| description | Diplodia seriata is a polyphagous and widespread pathogen that infects trunks, shoots, fruit and leaves of apple plants. Fungicides applied in integrated pest management programmes can act against D. seriata. However, introduction of scab-resistant apple cultivars and the consequent reduction in fungicide applications under low input disease management may increase the incidence of disease caused by D. seriata. Despite potential outbreaks of trunk canker, no fast and reproducible protocols for artificial inoculation of D. seriata and fungicide efficacy tests are available for apple plants. In this study, protocols for mycelium plug- and conidium suspension-inoculation of apple stems and shoots were optimised; canker disease assessments were carried out on potted apple plants under greenhouse conditions and coupled with D. seriata DNA quantification with quantitative PCR (qPCR). Efficacy tests of commercial fungicides showed that captan, dithianon and fluazinam inhibited D. seriata mycelium growth and conidium viability in vitro, while penconazole and ziram did not. However, dithianon spray applications did not reduce trunk canker severity and amount of D. seriata DNA in artificially inoculated plants under greenhouse conditions. This optimised protocol for fast and precise assessment of fungicide efficacy is suitable for further investigating the effects of other fungicides against D. seriata. |
| first_indexed | 2024-12-12T05:18:43Z |
| format | Article |
| id | doaj.art-e1b1283bd2244e8ba46e7e1e821d623b |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-12-12T05:18:43Z |
| publishDate | 2019-05-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-e1b1283bd2244e8ba46e7e1e821d623b2022-12-22T00:36:42ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952019-05-0158110.14601/Phytopathol_Mediterr-23764A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditionsElena ARRIGONI0Claudia Maria OLIVEIRA LONGA1Dario ANGELI2Matteo SOINI3Ilaria PERTOT4Michele PERAZZOLLI5Department of Sustainable Agro-ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, Italy Department of Agricultural and Environmental Sciences, University of Udine, via delle Scienze 206, 33100 Udine, ItalyDepartment of Sustainable Agro-ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, ItalyDepartment of Sustainable Agro-ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, ItalyDepartment of Sustainable Agro-ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, ItalyDepartment of Sustainable Agro-ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, Italy Center Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38010 San Michele all’Adige, ItalyDepartment of Sustainable Agro-ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, ItalyDiplodia seriata is a polyphagous and widespread pathogen that infects trunks, shoots, fruit and leaves of apple plants. Fungicides applied in integrated pest management programmes can act against D. seriata. However, introduction of scab-resistant apple cultivars and the consequent reduction in fungicide applications under low input disease management may increase the incidence of disease caused by D. seriata. Despite potential outbreaks of trunk canker, no fast and reproducible protocols for artificial inoculation of D. seriata and fungicide efficacy tests are available for apple plants. In this study, protocols for mycelium plug- and conidium suspension-inoculation of apple stems and shoots were optimised; canker disease assessments were carried out on potted apple plants under greenhouse conditions and coupled with D. seriata DNA quantification with quantitative PCR (qPCR). Efficacy tests of commercial fungicides showed that captan, dithianon and fluazinam inhibited D. seriata mycelium growth and conidium viability in vitro, while penconazole and ziram did not. However, dithianon spray applications did not reduce trunk canker severity and amount of D. seriata DNA in artificially inoculated plants under greenhouse conditions. This optimised protocol for fast and precise assessment of fungicide efficacy is suitable for further investigating the effects of other fungicides against D. seriata.https://oajournals.fupress.net/index.php/pm/article/view/5805Botryosphaeria obtusaMalus domesticaapple stem cankerDiplodia infection protocol |
| spellingShingle | Elena ARRIGONI Claudia Maria OLIVEIRA LONGA Dario ANGELI Matteo SOINI Ilaria PERTOT Michele PERAZZOLLI A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions Phytopathologia Mediterranea Botryosphaeria obtusa Malus domestica apple stem canker Diplodia infection protocol |
| title | A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions |
| title_full | A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions |
| title_fullStr | A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions |
| title_full_unstemmed | A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions |
| title_short | A fast and reliable method for Diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions |
| title_sort | fast and reliable method for diplodia seriata inoculation of trunks and assessment of fungicide efficacy on potted apple plants under greenhouse conditions |
| topic | Botryosphaeria obtusa Malus domestica apple stem canker Diplodia infection protocol |
| url | https://oajournals.fupress.net/index.php/pm/article/view/5805 |
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